摘要:

The products of the HLA‐D region and their correlation to psoriasis vulgaris was studied using the primed lymphocyte typing (PLT) assay. In families with one healthy and one psoriatic parent and one psoriatic child primary MLC (mixed lymphocyte culture) stimulation was carried out between responder cells from the healthy parent and stimulator cells from the psoriatic child. In this way 21 PLT reagents directed against putative psoriasis‐associated lymphocyte activating determinants were produced. Three reagents that gave clear bimodal stimulation patterns against lymphocytes of 51 psoriasis patients were further tested against 78 controls. Specificity of these reagents was studied using homozygous typing cells (HTC's) as stimulators.Compiled data show that cells from DR7 positive psoriatic patients give higher res‐timulation of these PLT reagents than do cells from healthy DR7 positive controls. In addition, a higher frequency of psoriasis patients compared to controls gave significant restimulation. Therefore we concluded that these PLT reagents recognized at least partly different DR7 associated determinants in the psoriasis patients and in controls. The reason is either that psoriasis patients carry a different lymphocyte activating DR associated specificity or, alternatively, that restimulation was caused by products of a distinct psoriasis associated locus in linkage disequilibrium with D/DR7 or of a determinant recognized together with D/DR7 as a restriction element. These data further support the notion that psoriasis is a disease with primary HLA associations to both class I and class II MHC

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb01491.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Serological analysis of the reactivity of a murine monoclonal antibody, HA113, towards lymphocytes from a random panel of 85 cell donors, and members of four families, indicated that HA113 recognizes a public specificity, which is different from the classical Bw4 antigen as defined by human alloantisera.

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb01492.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Sera from multiparous women and renal transplant recipients were screened for the presence of anti‐monocyte antibodies. Reactions caused by anti‐HLA antibodies were excluded by using panel cells that were HLA‐compatible with the antibody producers.Six sera with monocyte‐specific antibodies were identified, and at least two monocyte‐specific (probably allelic) antigens could be recognized.The perspective of the clinical significance of typing for monocyte‐specific antigens i

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb01493.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Polymorphisms among HLA class II molecules expressed by cells with different HLA‐DR4 haplotypes were analysed biochemically (isoelectrofocussing and 2D gels), cellularly (HLA‐Dw) and serologically (monoclonal antibodies). The results confirm the correlation which exists between HLA‐D specificity and DR beta chain isoelectric point polymorphism.Furthermore, a biochemical polymorphism was observed among DQw3 molecules. No correlation was found with HLA‐Dw types. On the other hand a correlation was found between DQ‐polymorphism and TA10 and 2B3 specificities defined by monoclonal antibodies. The comparison of different methods defining polymorphisms of HLA class II molecules will be

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb01494.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

We studied the influence of HLA class I and class II antigens on the suppression of the MLR induced by primed lymphocytes (PLs) alloactivatedin vitro.The suppression of 14 different PLs of 83 MLRs was analyzed. The PLs were primed against (i) HLA‐DP (SB) (ii) HLA‐DR/DQ or (iii) both HLA‐DP and DR/ DQ. The suppression was analyzed with special reference to the sharing of HLA‐antigens between (i) the stimulator in the MLR and (ii) the stimulator generating the PL.HLA‐DP and HLA‐DR/DQ antigens were equally capable of generating suppressor cells. When these cells were added to MLRs, the specific stimulators induced the strongest suppression (74%), while allogeic cells sharing class II antigens induced a slightly weaker suppression (66%). The suppression related to HLA‐DP (60%) was almost identical to that related to HLA‐DR/DQ (59%). The HLA‐A, B, C related suppression was of the same magnitude (58%). The unspecific suppression (40%), i.e. no relation to known HLA‐antigens, was significantly lower than the class II related suppression, but not significantly lower than the class I related suppression. The suppression of the MLR did not seem to be caused by cytotoxic cells, consumption of lymphokines, nor changes in the kinetics of the MLR. Thus, HLA‐DP antigens can ‐ like DR/DQ antigens ‐ induce PLs with the ability to suppress the MLR in an HLA‐class II (DP or DR/DQ) related, and possibly a class I related, as we

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb01495.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

HLA typing of an HLA‐B/GLO recombinant family using bulk‐expanded DP(SB) alloreactive T‐cells (GNN1 to 6) as well as conventional HLA‐ABC, D, DR. and DQ typing showed that a paternal recombination had taken place between HLA‐DR and DQ on the one hand and HLA‐DP(SB) on the other. The recombinant child wasDPw4, 6‐heterozygous and differed in terms of class II determinants only for DPw6 from two otherwise HLA identical siblings, who were probablyDPw4/4‐homozygous. These siblings did not stimulate the recombinant in MLC whereas they responded to his cells indicating that DPw6 can stimulate in primary MLC. Moreover, it was possible to generate and bulk‐expand DPw6‐reactive lymphocytes (PLs) between MT and MA as responders and BN as stimulator. The correlation coefficient (r) between the reaction of these PLs and the GNN6 (anti‐DPw6) reagents was 1.0 when tested against a panel of 71 individuals.This is the first report demonstrating that the DPw6 gene, like the DPw1‐w5 genes, is located between DR/DQ and GLO.The frequency of the DPw6 antigen in 41 unrelated, randomly selected Danes was 7%. HLA‐DP typing of the BN family revealed that the DPw6 positive but not DPw6 negative family members gave intermediate responses with one (GNN2B) but not with another (GNN2A) anti‐DPw2‐reagent. Studies of 63 healthy individuals (unrelated to the BN family) revealed seven similar discrepancies between GNN2A and GNN2B. Strikingly, all four DPw6 positive stimulators gave rise to such discrepancies indicating that the GNN2B reagent is cross‐reactive and recognize a common part of the DPw2 and DPw6 molecules. No cases of GNN2

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb01496.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

When a number of families can be identified with two or more siblings having a particular disease, Green&Woodrow proposed a test for MHC‐disease association. An extension of this test is presented to detect more general associations when family sizes exceed thre

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb01497.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

When a number of families can be identified with two or more siblings having a particular disease, Green&Woodrow proposed a test for MHC‐disease association. An extension of this test is presented to detect more general associations when family sizes exceed thre

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb01498.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY