摘要:

MLC inhibition studies were performed with two human alloantisera: one specific for HLA‐B7, the other for HLA‐DRw7. The stimulator cell inhibitory effects of these sera were tested in primary and secondary MLC tests. Both sera inhibited in the primary MLC, whereas only the anti‐DRw7 serum was capable of blocking the secondary MLC test. The difference in inhibiting properties of these sera was further analyzed in primary MLC tests using selected MLC combinations, Fc receptor negative cell populations and pepsin digests of the anti‐B7 serum. Anti‐DRw7 antibodies could inhibit by masking the stimulatory DR antigens. The inhibition of the anit‐B7 antiserum was dependent on Fc, which suggested that anti HLA‐B antibodies inhibited by some other mechanism. This inhibition could have been caused by antibody dependent cellular lympholysis of the stimulator cells or by the induction of suppressor

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1980.tb00878.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Fourteen HLA‐A and 18 HLA‐B antigens were studied in three samples of Pyrenean populations: 198 unrelated individuals of a “Pays Basque” group; 212 non‐Basque individuals from a valley in Beam, l'Ouzom; and 73 non‐Basque individuals from the neighboring valley of Bareges.The results in the Basque and the non‐Basque people from l'Ouzom were comparable: the gene frequencies of HLA‐A29, Aw19.2, B17 were increased and the haplotypes HLA ‐ Aw 19.2, B18; A29, B12; A2, B5; Al, B17 were found frequently with a striking linkage disequilibrium; HLA‐B18 had an increased gene frequency in all these Pyrenean populations, while Bw35 was frequent in l'Ouzom and Bareges, but not among the Basques. The characteristics of Bareges were very different: the gene frequencies of HLA‐A2, All, B7 were increased while the frequency of HLA‐B5 was low; the most characteristic haplotypes were HLA‐A2, B12; A2, B18; All, Bw35; All, B27.It is interesting to note discrepancies between ethnic and HLA classification of the Basques and the non‐Basque population of l'Ouzom. The HLA characteristics are quite different in the Bareges sample, more closely resembli

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1980.tb00879.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Acidic eluates from two rejected kidney allografts contained antibodies reacting only with the endothelial cells and the monocytes of the specific kidney donors. No reaction was observed with the B‐cells or the T‐cells of these donors.When these eluates were tested against the leukocytes of healthy blood donors that were typed for HLA‐A, ‐B, ‐C and DR, positive reactions were observed with the monocyte but not with the lymphocyte fraction. The first eluate reacted with 6.4% of the donors and the second eluate with 38.3%. No correlation with any of the HLA‐A, ‐B, ‐C or DR antigens was found.If a more extended study proves that the endothelial‐monocyte antigens do play an important role in kidney transplantation, matching of donors and recipients for these antigens and crossmatching recipient serum with donor monocytes may become a necess

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1980.tb00880.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

The minimal sequential absorption of 12 HLA‐B14 antisera with B14 positive cells provided 10 antisera that showed a high correlation (r values<0.8) with the original B14.1 and B14.2 sera of Dewar et al. (1977). The subdivisions defined by the absorbed sera corresponded to the B14 subtype of their immunizing cell. The subtyping of 112 B14 positive individuals showed the frequency of B14.1 to be 33.04%. B14 heterozygous individuals always typed for one but never for both subdivisions. Analysis of a population of 2,044 showed B14.1 to be in linkage disequilibrium with Aw32 and B14.2 with Aw33, Aw30 and A3. It is concluded that B14.1 and B14.2 are distinct antigens which, in the population tested, comprise the entire specificity of HLA‐

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1980.tb00881.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Antisera against the products of the major histocompatibility system (MHS), produced by immunization between congenic mouse strains differing only at that segment of the 17th chromosome which bears the H‐2 gene complex, exert a reproducible blocking effect on formation of rosettes with EACm(C3d rosettes). In many instances this effect is probably due to specific antibodies reactive with the lymphocytes, as indicated by immunochemical and adsorption experiments. Purified antibodies from the C3H anti‐C3H.B10 immune ascites failed to exert this blocking effect. The blocking capacity can be restored, however, by addition of fresh normal mouse, rabbit or fetal calf serum, but not by heat inactivated serum.These experiments show that the presently defined H‐2K, H‐2D and H‐2L antigens as well as some la antigens are themselves not the C3d receptors. The blocking effects observed in the presence of complement are possibly due to the proximity of H‐2 or la molecules and C3d receptors, or to a rearrangement of membrane components after reaction with anti‐MHC antibodies a

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1980.tb00882.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

The usefulness of Primed Lymphocyte Typing (PLT) for recognizing Lymphocyte Defined (LD) determinants of die Major Histocompatibility Complex of the dog (DLA) was assessed in 10 separate experiments. Application of a technique described for human cells to peripheral blood lymphocytes of dogs gave reproducible, informative results. DLA LD determinants were shown to be of major importance in secondary responses of alloantigen “primed” lymphocytes, but other, as yet undefined, factors also appeared to play a role in the as

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1980.tb00883.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

A human alloantiserum was found which selectively inhibits responding cells in mixed leukocyte culture reactions. Inhibition was achieved by pre‐incubation of responder cells in the antiserum followed by washing. The serum showed dual specificity as an inhibiting agent. First, inhibition was restricted to HLA‐B7 or ‐B40 positive stimulator cells, specificities against which the antiserum also had cytotoxic activity. Second, inhibition was almost exclusively associated with the presence of the phenotype HLA‐Al, ‐B8 on the responder cellsThe HLA associated specificity for responder cells was unexpected since no alloantibody activity directed to responder alloantigens could be detected by conventional serological methods. The antiserum donor had not been immunized with HLA‐Al, ‐B8 antigens nor with known crossreactive antigens. Furthermore, the serum donor did not carry HLA‐Al, ‐B8 antigens herself.The inhibiting substance in the antiserum had physicochemical properties of IgG and was specifically reactive with HLA‐B7 positive platelets. Pepsin digest preparations were not inhibitory. Fc receptor positive responder cells were required for inhibition. Responder cells, preincu‐bated with the antiserum, suppressed the response of cells not incubated with the antiserum. Three possible explanations of these results are discussed: specific binding of the Fc part of the antibody with Fc receptors of responder cells, specific activation of suppressor cells

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1980.tb00884.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

HLA‐A,B,C phenotyping was performed on 95 patients with geographic tongue to determine whether there is an increased frequency of any particular allele in this condition. An increased frequency of B15 was found in the patients when compared to normal controls. When the patients were divided into atopic and non‐atopic groups there was an increased frequency of B15 and a decreased frequency of B40 in the atopic group compared to the controls. B40 was also decreased in the atopic group when compared to the non‐atopic group. When the type I correction factor was applied to the probability values the differences in antigen frequencies in all cases became insignif

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1980.tb00885.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

The expression of H‐Y antigen on preimplantation mouse embryos has been studied by complement dependent cytotoxicity. Embryos at the 8‐cell stage were exposed to anti‐H‐Y and complement and then scored for cell death. Overall, half of the treated embryos were killed, retarded, or contained dead cells. Of those embryos which were not affected by anti‐H‐Y, 92% were female. In control experiments, neither normal serum plus complement nor complement alone had any specific effect on male embryos. It is concluded, therefore, that the H‐Y antigen is present on preimplantation

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1980.tb00886.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

In 41 patients with AP and 134 healthy relatives HLA haplotypes and total hemolytic complement and C2 concentrations were determined. Though complement levels were found in the lower normal range in none of the individuals studied, a genetically determined C2 deficient state could be established. No association between low C2 levels and the HLA haplotype A10(A25), B18 or an isolated A25 or B18 was observed. A comparison of phenotype frequencies of AP patients with controls showed no significant difference. Contingency table analysis of patients and control haplotypes showed a definite close association with HLA‐Al, Bw22, A2, Bw16, and A29 B1

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1980.tb00887.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY