摘要:

Normal human lymphocytes isolated from freshly drawn blood were repeatedly stimulated (up to five times) in mixed lymphocyte cultures (MLC) with five different Epstein‐Barr virus (EBV)‐transformed homozygous typing cells (HTC‐LCL). Each time, the same WDW‐specificity was used for the restimulation. The first and the second stimulations resulted in an 2.4‐to 3.1‐fold increase of the original responder cell number. But after the third, fourth and fifth stimulation decreasing amounts of specifically primed lymphocytes were recovered. Primed lymphocyte typing (PLT) showed significant differences in the intensity of the stimulation depending on whether homozygous typing cells (HTC) or HTC‐LCL were used as stimulators. Except for one cell type, the PLT‐response was significantly stronger with HTC‐LCL than with HTC. The time needed to reach the maximal PLT‐response got shorter and shorter the more often the cells were reprimed by the same stimulator cell. Further differences between HTC and HTC‐LCL were observed in the complement‐dependent cytotoxicity assay: HTC‐LCL were more sensitive towards rabbit complement and they showed an enhanced binding of anti‐Ia‐alloantibodies but HTC reacted more spe

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01131.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

During the VIIth Histocompatibility Workshop, 44 sera were selected that defined B‐cell alloantigens showing correlation with the HLA—D antigenic determinants. The segregation of these antigens, now called DRw (HLA—D‐related), was studied in a family that contained one HLA‐A/B recombinant and one HLA–B/D recombinant sibling.It could be established that the DRw‐1 antigenic determinants segregated with the B‐D region in the A/B recombinant sibling and that the DRw‐2 antigen segregated with the D region of the B/D recombinant child. In the second family, DRw typing showed that one of the children was identical with an HLA–ABC non‐identical sibling. This suggested that a crossing‐over had occurred. This was confirmed by mutual non‐stimulation of these HLA‐ABC non‐identical children in the mixed lymphocyte culture. This crossing‐over involved the DRw‐3 alloantigen.These data confirm the assumption that serologically defined B‐cell alloantigens are coded for by genes located outside the HLA‐A/B region on the B‐D side of the chromosome. Moreover, these data suggest that, within families, serological B‐cell genotype identity can predict a mutual unresponsiveness in the lymphocyte culture.However, findings in these two families indicate that discrepancies nevertheless exist between B‐cell serology and mixed lymphocyte reactions and that mutual stimulation can occur despite apparent identity of serological B‐cell phenotype and that B‐cell phenotype identity does not necessarily predict mutual u

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01132.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Complete HLA typing including HLA—A, —B, —C, —DR (D related B cell typing), —D, mixed lymphocyte culture (MLC), and primed lymphocyte testing (PLT), together with complete red blood cell (RBC), glyoxalase (GLO), GBG (Factor B), and phosphoglucomutase 3 (PGM3) typings were performed on an informative family. The five siblings inherited the four possible combinations of parental HLA haplotypes, and two of the siblings were HLA—A, —B, —C and —DR identical. Repeated MLC testing of the family revealed positive mixed lymphocyte reactivity in all combinations. B cell typing for the DR specificities demonstrated no variation from the expected inheritance pattern and specifically no recombination event. GBC and GLO typings militated against a recombination involving the paternal chromosome. HLA‐D testing revealed that only one of the HLA—A, —B, —C, and —DR identical siblings gave typing responses to the HLA—Dw3 specificity present on that maternal haplotype. Utilizing HLA haploidentical combinations, lymphocytes were primed against the four parental haplotypes and the non‐Dw3 haplotype of interest (Aw24—B8—DRw3—LDY) in the PLT. The sibling inheriting this haplotype did not restimulate cells primed against the A2—B40—DRw6—LDY specificity. Furthermore, no discrimination was observed in the restimulation of lymphocytes primed against this haplotype. Possible interpretations of these family data include: a spontaneous mutation, non‐major histocompatibility locus

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01133.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Responses of approximately 200 persons were measured by mixed leukocyte cultures against 41 lymphoblastoid cell lines which were thought to be homozygous for HLA—D (i.e. LCL‐HTCs). These responses were standardized by computer analysis and the resultant Interaction Indices for each LCL‐HTC were compared with those of every other LCL‐HTC in a correlation matrix of 861 independent analyses. Only those analyses involving LCL‐HTCs of defined HLA—D types are reported here. In general, these studies confirmed many allelic relationships of reputedly similar LCL‐HTCs; however, several unexpected groups of correlations suggested that groups Dw1 and 3 overlap to form Groups “1a‐3,”“1b‐3” and “true 3.” The “1b‐3” group also included Dw8. Segregation of the “1b‐3” allelic group was also observed in a family study. These preliminary analyses suggest a working model for further investigation of HLA‐D allelic relationships (Fig. 3). On the other hand, they may also be interpreted to suggest that “typing” with LCL‐HTCs may be controlled by a separate HLA locus whic

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01134.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Antisera directed against histocompatibility‐2 antigens of the mouse suppress the formation of spleen colonies by pluripotent hemopoietic stem cells. Of antisera which are specific for subregions of the H‐2 complex, only those which contain anti‐H‐2K or anti‐H‐2D activity are effective. Specific anti‐Ia sera do not react with the stem cell. If the titer of the CFU‐s suppressive effect of the antisera is compared to their toxicity to spleen lymphocytes, it can be concluded that CFU‐s express antigens in the same density as spleen lymphocytes. The amount of H‐2 antigens expressed on the surface of CFU‐s is independent of its

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01135.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01136.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Forty‐seven patients with a “definite” or “classical” rheumatoid arthritis according to the ARA criteria were typed for the serologically detectable HLA—A, —B, and —C antigens and 36 of these patients were typed for the HLA—D antigens, Dw1, 2, 3, 4, 6, 7, and 8 by the MLC technique.The frequency of Dw4 was increased to 44.4% in the patients compared to 17.2% in normal controls (P= 8 × 10−4). The frequency of Dw1 and Dw7 was also increased although this was only of borderline significance. The frequency of Dw2 was remarkably low, especially in females, which is of interest, as the same antigen has a low frequency in some other autoimmune diseases.No significant deviations of the frequencies of HLA—A, —B, and —C antigens were found in rhe

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01137.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01138.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01139.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01140.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY