摘要:

The histogenesis of the intradermal melanocytic tumor induced in the skin of 88 female BDF1mice by DMBA and TPA is studied light‐and electron‐micro‐scopically. Four types of melanocytes were found in the mouse skin. [1] The epidermal melanocytes transiently appeared several days after topical application of DMBA. [2]Hair follicles turned into anagen phase and follicular melanocytes became apparent by week 3. [3] After week 3, intradermal melanocytes of the perifollicular melanocytic networks (PFM), which were scattered in untreated mouse skin, proliferated to form the intradermal melanocytic tumors, and 267 tumors, composed of oval melanocytes in medullary growth, were induced in all of the 19 treated mice by week 32. [4]Large dendritic melanocytes located deep in the reticular dermis, which did not participate in the formation of the tumor. Only 3 papillomas were induced in 2 of the 19. Light‐and electron‐microscopically, there was no evidence of migration of epidermal and hair follicular melanocytes into the dermis. Additionally, melanin‐producing activity in the cytoplasm of Schwann cell and perineural epithelium was observed. The histogenesis of the intradermal melanocytic tumor was closely related to the melanocytes of the perifollicular melanocytic ne

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01455.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

MTV antigens in the resting mammary glands of GRS/A, SHN, C3H, and Balb/c virgin mice were detected by immunoperoxidase techniques using antiserum against MTV whole virion, gp 52 or p 25 to differentiate the expression between endogenous and exogenous MTV. Balb/c mice were crossed to infect MTV into each reciprocal hybrid or fosternursed inbred. Immunohisto‐chemical stainings of gp 52 in the formol‐fixed sections were almost the same as those of the whole MTV virion, and the results on various cases were as follows: In the mice with endogenous GR‐MTV, positivity was first observed at the age of 14 days, while the first expression of exogenous GR‐MTV was delayed to the age of 140 days. The mice with endogenous and/or exogenous SHN‐MTV showed the first antigen appearance at the age of 65 days, and those with exogenous C3H‐MTV did at the age of 80 days. The virgins with only endogenous C3H‐MTV came to express the antigen after the age of 200 days. Staining of p 25 in Carnoy‐fixed sections of MTV‐positive mammary glands was found in the supranuclear cytoplasm and apical surface of the glandular cells and the lumen, all of which are the site of A and B particles. By means of preembedding method for gp 52, the reaction products were ultrastructurally detected not only on the MTV‐budding apical surface, together with the intraluminal B particles, but also on the MTV‐free apical cell membrane of the glandular cells in the mammary gl

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01456.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

In vivoeffects of anti‐asialo GM1(GA1) serum on hemopoiesis were studied by morphological examination of the blood, bone marrow, and spleen and by measuring the number ofgranulocyte‐macrophage colony‐forming units(CFU‐GM) per femur after injecting of the anti‐serum into mice intraperitoneally. The number of circulating lymphocytes markedly decreased 2 days after injection and then recovered, but circulating granulocytes gradually declined and reached a nadir at 6 days, then rapidly recovered. Granulopoietic activity in the bone marrow was reduced on day 2, but recovered profusely from day 4, followed by marrow neutrophilia on day 10. The spleen showed marked extramedullar granulopoiesis on days 6 and 10. CFU‐GM per femur increased by 24 hours, reaching a maximum value 250% of normal on day 3, then returned to the normal level. CFU‐GM growth was not affected by the treatment of anti‐GA1serum plus complementin vitro.These results suggest that the proliferating compartment of the myeloid series in the bone marrow was eliminated by anti‐GA1serum injection that led to a decrease in granulocytes and an i

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01457.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The wave line is a structure that is frequently observed in aorta. Grossly this structure (wave line), measuring 0.3 0.5 cm in width and 5–10 cm in length, runs longitudinally or spirally along the long axis of the aorta. The distribution and the relation with arteriosclerotic plaque of wave line were studied. The wave line had three predilection sites: A) the wave line running down from closure of Botallo duct to ostium of 6–8th intercostal artery, B) the wave line between ostium of both renal arteries, C) the wave line running down from lumbar artery to iliac arteries. The wave line observed in the aortae of neonates gradually increased with age. Its frequency is about 80–100% in the second and the third decades. The association of arteriosclerosis with the wave line was frequently observed, especially on “C” region. Fifty‐five percent of arteriosclerotic plaques in the fourth decade corresponded with th

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01458.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The correlation between increased serum alpha‐fetoprotein (AFP) and a human vitelline component of 5 testicular embryonal carcinomas (1 was one histological type, 4 were more than one histological type) with no histological features specific for yolk sac tumor (YST) or endodermal sinus tumor (EST) was presented. In all 5 cases, distinct cells simulating the human yolk sac endodermal cell (HYSEC) with eosinophilic‐granular or clear‐vacuolated cytoplasm were found. These cells contained PAS positive, diastase resistant eosinophilic hyaline globules (EHG) which were positive for AFP. In the embryoid body, the same cells with positive AFP as the HYSEC were also seen. Electron microscopic investigation of these cells revealed basement membrane‐like materials which were observed in organoid structure of YST. Positive AFP in these cells showed no binding to Concanavalin A (Con A), which was similar to AFP in YST. It was suggested that testicular embryonal carcinoma with higher serum AFP showing no histological features specific for YST had a vitelline component simulating the HYSEC and produced an increased serum AFP. Immunohistochemical staining for AFP of embryonal carcinoma with higher serum AFP is useful for detecting a human vitelline component and is important to guess the survival for the

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01459.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Distribution and fine structure of macrophages were studied in 10 human embryos in the 6th and 7th week of gestation, 5.5 to 12 mm in crown‐rump length. The yolk sac macrophages were found in the extravascular mesenchymal tissues and intravascular spaces long before the first appearance of bone marrow and lymphatic tissues in the embryos. In addition to the macrophages, the fibroblastic cells and the cells of erythropoietic series were also present in the extravascular space. The macrophages showed a variety of cellular structures suggesting transition from immature cell type with no heterophagolysosomes to mature cell type in phagocytosis. The mature macrophages avidly phagocytized the primitive erythroblasts and occasionally platelets. They were positively stained for lysosomal enzymes and were characterized by numerous pleomorphic heterophagolysosomes which exhibited various stages of digestion of phagocytized blood cells. The origin of intravascular macrophages may be in either migrated extravascular macrophages or phagocytic endothelial cells. The phagocytosis and degradation of erythroblasts appear to be one of the main functions of yolk sac macrophages. The presence of the macrophages in mitosis indicates their proliferationin sit

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01460.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

In an attempt to elucidate various histological features of gastric cancers, seven human gastric adenocarcinomas were studiedin vitroand in nude mice. Growth pattern of each cultured cell linein vitrocorresponded well to the histological type of parent tumor. The cell lines, MKN7, MKN74, and MKN28 derived from differentiated carcinomas showed morphological characteristics of intestinal differentiation in cell polarity and microvilli with core‐filamentsin vitroas well as in nude mice. However, they gradually diminished the characteristics in course of time. The cell lines, MKN 45 and OKAJIMA, derived from undifferentiated carcinomas, had natures of not only ordinary gastric mucosa but also intestinal metaplastic mucosa. They seem to have multipotentiality for differentiation, and preserved well the natures for long periods of culture. The KWS‐I cell line composed of undifferentiated cellsin vitrodisplayed the potential for differentiation in nude mice. However, the differentiation of KATO‐III cells derived from a signet‐ring cell carcinoma was suppressed in nude mice. The common abnormality of chromosome was not found, and the growth ratein vitrowas not dependent on the histological type of paren

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01461.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Two adrenocortical adenomata with Cushing's syndrome were examined employing cell culture methods. The results revealed that the clear‐type cells changed into compact‐type cells, and that the compact cells played a role in the production and secretion of steroid hormones. Furthermore, it is likely that they underwent fragmentation and produced collagen fibr

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01462.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The immunohlstochemical localization of three triplet proteins of neurofilaments in normal Kultschitsky cells and tumourlets of the human lung has been studied using avidin‐biotin‐poroxidase complex (ABC) method. Kultschitsky cells and tumourlets have been stained with antisera against 68 K, 150 K and 200 K dalton components of the nourofilaments, respectively. Ultra‐structural observations of human Kultschitsky cells have revealed the presence of bundles of intermediate filaments as well as microtubules and neurosecretory‐type granules. In the tumourlets, similarly sized filaments were found, but were relatively scarce. Since intermediate filaments are thought to be specific to differentiated cells and neurofilament proteins are restricted to the neuronal tissues, we conclude that Kultschitsky cells of the lung are of neuronal

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01463.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Mallory body‐like structures (MBLs), which resemble Mallory body (MB) in the liver, were observed in lungs of 4 cases showing interstitial pneumonitis. MBLs were found in the cytoplasm of especially swollen alveolar epithelial cells. MBLs and MBs had almost similar characteristics morphologically and tinctorially under the light microscopy. MBLs were composed of intermediate type fine fibrils which were positive for anti‐keratin antibody. These findings suggested that MBLs were a kind of pathological keratinization of alveolar epithelial cells. Although the origin of MBL containing cells could not be clarified, they were closely related to type 2 alveolar epithelial ce

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01464.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY