摘要:

Nodular lesions induced by N‐nitrosobis (2‐hydroxypropyl) amine (BHP) and phenobarbital (Pb) in F344 rat thyroid were histologically divided into 2 categories, benign lesions and carcinoma. Benign lesions were subclassified into 3 types mainly based on histological characteristics and localization of peroxidase (PO). The type 1 lesion showed localization of PO almost identical with that of the group 1 rat thyroid. Type 2 was characterized by an intense reaction product of PO in the cytoplasm but a weak one on the surface of the microvilli, and type 3 exhibited localization of PO opposite to that of type 2. Carcinoma proliferated in papillary, follicular and poorly differentiated patterns and developed asde novocarcinoma with the highest incidence, although a considerable number of carcinomas coexisted with benign type 3 lesions. A majority of these carcinomas lacked the reaction product for PO. These results suggest that benign nodular lesions, especially types 2 and 3, show hyperactivity of PO synthesis reflecting their response to excessively secreted TSH, whereas carcinoma is autonomous, because of marked inhibition of PO synthesis even in the presence of TSH hypersecretion. Acta Pathol Jpn 40: 1–8

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01522.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

In order to determine the influence of strain and sex on local carcinogenesis in rat mammary tissue, 1 mg of 7, 12 dimethylbenz(a)anthracene (DMBA) was dusted directly onto the exposed mammary gland of 30‐day‐old Long‐Evans (L‐E) rats and Sprague‐Dawley (S‐D) rats. The experiment was terminated 28 weeks after application of the carcinogen. Tumors measuring between 1 and 2 cm in diameter were harvested from female L‐E rats with high frequency (85%) and long latency (mean: 23.7 weeks after DMBA dusting), and from female S‐D rats with extremely high frequency (98%) and short latency (16.7 weeks). Male rats of both strains were almost identically much less susceptible to DMBA (L‐E; 55%, 25.0 weeks, S‐D; 53%, 23.9 weeks). Ovariectomized S‐D (47%, 24.9 weeks) and orchiectomized S‐D (30%, 24.8 weeks) rats, which were gonadectomized at 30 days of age, respectively, were also much less susceptible. A variety of histologies, mostly malignant epithelial, mesenchymal or mixed tumors, were noted in each group. The carcinomatous response in the mammary tissue was much higher in female S‐D (96%) than in female L‐E (50%) rats, and very low in male and gonadectomized rats (10‐20%). In contrast, the sar‐comatous response in the mammary tissue was moderate in female and male L‐E and male S‐D (43‐50%) rats, and low in the other groups (15

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01523.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Estrogen receptors (ER) in human gallbladders were examined immunohistochemically using a specific monoclonal antibody against human ER. ER immunoreactivity was positive in 6 (19.4%) of 31cases of cholelithiasis, 6 (33.3%) of 18 cases of epithelial polyp, 14 (53.8%) of 26 cases of adenoma, and 26 (22.8%) of 114 cases of adeno‐carcinoma. ER immunoreactivity was located at the nucleus of the epithelium of both non neoplastic and neo‐plastic tissues, although the number of ER‐immunoreactive cells was small. There was no sex difference in the incidence of ER immunoreactivity. No significant difference in survival rates could be demonstrated between ER‐positive and ER‐negative carcinomas. Epithelial polyps, adenomas and carcinomas were divided into metaplastic type and non‐metaplastic type based on the presence or absence of metaplastic changes for comparison of the incidence of ER immunoreactivity. The incidence of ER immunoreactivity in the metaplastic type was significantly higher than that in the non‐metaplastic type. We conclude that ER are present in the gallbladder mucosa in various disease states and suggest that the presence of ER is related to metaplasia of the gallbladder mucosa. Acta Pathol Jpn40

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01524.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Expression of epidermal growth factor receptor (EGF‐R) in human hepatocellular carcinoma (HCC), hepatoblastoma and non‐cancerous liver tissues was investigated immuno‐histochemically in order to evaluate the possible role of EGF‐R expression in neoplastic transformation of he‐patocytes. lmmunoreactive EGF‐R molecules were identified on frozen sections by means of the avidin‐biotin im‐munoperoxidase complex technique using a monoclonal antibody recognizing an epitope of the external domain of human EGF‐R. Linear positive staining was present on the surface of carcinoma cells in one hepatoblastoma and in 9 of 11HCCs. In addition, an enhanced level of surface EGF‐R expression was observed on the tumor cells in 9 of 12 cases in comparison with that on hepatocytes in surrounding non‐cancerous liver tissue, which in most cases showed chronic inflammation, hepatocyte injury or regeneration. No positive staining in the form of coalescent cytoplasmic granules was present in HCC or hepatoblastoma cells, nor in the cytoplasm of hepatocytes in normal or non‐cancerous diseased liver tissue. Little or no reactivity was present on the surface membrane of hepatocytes in the normal liver tissues of 8 control cases. Furthermore, immunoelectron microscopy revealed the localization of this immunoreactive EGF‐R molecule on the plasma membrane. Considering that the functional form of EGF‐R could be localized on the plasma membrane, the enhanced expression of immunoreactive EGF‐R on the tumor cell surface demonstrated here may suggest a possible role of EGF‐R in the development or progression of human HCC as well as in hepatocyte regeneration.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01525.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

An ultrastructural study of human pineal glands obtained at autopsy from 7 patients older than 70 years was conducted in order to clarify the functional anatomy of the pineal in the aged. By light microscopy, the pineal glands from aged patients were parenchymatous and almost indistinguishable from those of younger controls. Electron microscopy of the pineal parenchymal cells revealed deep nuclear indentations, synaptic ribbons and ribbon fields, Golgi apparatus, lipofuscin granules and microtubular sheaves in all subjects, cilia with a 9+0 pattern in a few, and lamellated structures suggestive of the outer segment of photoreceptor cells very rarely. Microtubules were numerous in the cytoplasmic processes and bulbous endings. Fibrous astrocytes located between the pinealocytes showed long and thin cytoplasmic processes containing numerous glial filaments. Two types of nerve bouton were present in the pineal parenchyma, one of which contained clear vesicles forming synapse like contacts with pinealocytes. There were no significant age‐related changes in these features in a qualitative comparison with pineal glands from 5 adult patients younger than 70 years. These findings indicate that even in advanced age, the human pineal gland maintains some functions, such as intercellular communication and photoreception, in common with the pineal in lower vertebrates. Acta Pathol Jpn 40: 30–40, 1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01526.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

In order to investigate anthracosis of the human lung, especially its causal relationship with atmospheric pollution and the occurrence of lung cancer, intrapulmonary particulate pollutants (IPP) from autopsy cases and patients lobectomized because of lung cancer were separated by alkali digestion of the lung tissue, and their elemental constitution was analyzed by a wavelength‐dispersive X‐ray fluorescence spectrometer. Silicon was the most abundant mineral constituent of non‐carbonaceous fraction of IPP, followed by calcium, magnesium, iron, aluminum and other trace elements. The levels of silicon and aluminum in IPP were significantly higher in individuals treated at Saitama Medical School Hospital than in those from Tokyo. Farmers showed higher levels of silicon and aluminum than other occupational categories, whereas male blue‐collar workers showed higher levels of calcium and lead than farmers. The level of iron in IPP of male smokers tended to be higher than in non‐smokers. In cases of lung cancer, especially of the hilar type, the levels of iron, calcium, copper, lead, chromium and nickel in IPP tended to be higher than in non‐lung cancer cases, whereas the levels of silicon and aluminum were lower than in non‐lung cancer cases. On the basis of these results, pulmonary anthracosis was considered to be etiologically related to the occurrence of lung cancer. Acta Pathol Jpn 40

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01527.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A case of cerebellar medulloblastoma with clusters of mature ganglion cells and glial cells is described. The patient, a 15 ‐year ‐old girl, underwent three operations followed each time by radiation and chemotherapy during the four‐year clinical course. Histologically, the ganglion cells were clearly identifiable by their abundant eosino‐philic cytoplasm, round nuclei with prominent nucleoli, tigroid granules, and argyrophilic fibrils and axons. Im‐munohistochemically, the cells were NSE‐ and NF positive, and ultrastructurally they contained abundant tubules and filaments, neurosecretory granules and well developed rough endoplasmic reticulum. There were many cells transitional in appearance between primitive cells and mature ganglion cells. The tumor also had many mature yet atypical astrocytes and oligodendrocytes. The exact mechanism of the extensive neuronal and glial maturation of medulloblastoma cells is unclear, but the repetitive surgical interventions, radiation and chemotherapy might have had certain cytostatic effects on rapidly dividing medulloblastoma cells, giving them a chance to mature into postmitotic cells with potential for neuronal and glial differentiation. Acta Pathol Jpn 40:

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01528.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A case of peritoneal maligant mesothelioma in a radiation technologist, who had worked in this field for 34 years, is reported. Histopathologically, a biopsy specimen from the retroperitoneal tumor revealed a biphasic type of malignant mesothelioma. Electron microscopy disclosed that the tumor cells contained prominent microvilli, basal laminae adjacent to the stroma, junctional complexes, desmosomes, tonofilaments, clusters of glycogen granules, welt developed rough endoplasmic reticulum (RER), confronting cisternae showing direct continuity with the RER and membrane‐bound granules suggestive of secretory activity. No increased amount of asbestos was detected in autopsied lung material or the peritoneal mesothelioma. The estimated cumulative dose of occupational irradiation was calculated to be about 40 to 50 rad at most. Irradiation was discussed in relation to the etiology of the peritoneal mosothelioma. Acta Pathol Jpn 40: 57–62, 1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01529.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A 32‐year‐old Japanese man with chronic granulocytic leukemia died of respiratory failure. Autopsy revealed alveolar proteinosis and pulmonary fibrosis, complicated by disseminated atypical mycobacteriosis. Epithelial hyper‐plasia caused by busulfan therapy was probably responsible for the induction of excessive surfactant production, resulting in alveolar proteinosis, and the immunosuppres‐sive state due to chronic granulocytic leukemia was probably related to the induction of disseminated atypical mycobacteriosis. Acta Pathol Jpn 40: 63

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01530.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Testosterone secreting adrenal adenoma is rare. We recently experienced a 17‐year‐old pubertal girl who showed signs of virilization and a high serum level of testosterone. The excised adrenal gland showed a 3.5 × 3 × 3‐cm cortical adenoma. Light and electron microscopic findings together with the high serum level and high tumor tissue contents of testosterone and dehydroepiandrosterone (DHA) indicated that the tumor was a testosterone‐secreting adrenal cortical adenoma. This appears to be a rather rare tumor from a review of the literature. Interestingly, in this case, the cytoplasm of the tumor cells contained structures resembling spironolactone bodies. From the results of enzyme histo‐chemistry, the steroidogenetic pathways in this tumor were speculated. Acta Pathol Jpn 40:

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01531.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY