摘要:

The structure of buffy coat from normal and leukemia rats was studied. Normal buffy coat in a microhematocrit tube showed a distinct stratified structure consisting of leucocytes, platelets, and leucocyte debris from the bottom to the top. Two distinct layers were further identified in the leucocyte layer in many rats. They were composed of polymorphonuclear leucocytes (bottom) and lymphocytes (top). From the prominent change in the thickness of each layer during 7,12‐dimethylbenz(a)anthracene leukemogenesis, this technique was shown to be useful for screening, follow‐up, and classification of the leukemia in this experimental sys

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1973.tb00769.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

In order to determine the origin of brain macrophages, which are considered to be derived from “resting microglia of Hortega”, autoradiographic studies using3H‐thymidine were carried out to reveal the following results.Almost all the brain macrophages were proved to be derived from circulating leukocytes, especially from monocytes. Other types of cells, including “resting branched microglia of Hortega”, do not play any significant role in the brain macrophage system.Electron microscopic autoradiography revealed marked metamorphosis of monocytes at the vicinity of the lesion, and they were designated as transformed monocytes.On the base of these results and electron microscopic morphology of “resting microglia of Hortega”, the author concluded that Hortega's microglia system can be divided into two different cell groups. The first is that seen In a resting state of mature and immature animals and the second is that appearing in damaged brain. The former Is thought to belong to neuroectodermal glia and the latter to hemat

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1973.tb00770.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

Twenty renal biopsy specimens taken from 17 patients with anaphylactoid purpura were examined by light and electron microscope. Virus‐like microtubular inclusions were observed in the glomerular endothelium of 4 specimens from 3 patients. They consisted of interwoven microtubular structures measuring 200–280 Å and often appeared to be branched. Identical structures have been reported in patients with systemic lupus erythematosus and given the specificity for the diagnosis. However, the observations of apparently identical structures in patients with renal disease other than systemic lupus evoked a question to its specificity. The presence of the structures in patients with anaphylactoid purpura and systemic lupus erythematosus suggested some similarity of these two conditions. The exact nature of the structures is unknown at pre

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1973.tb00771.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

The reaction mode of the intraperitoneal cells of guinea pigs both to protein materials and to non‐protein materials has been studied, paying special attention to their molecular weights.In both groups of guinea pigs, non‐sensitized and sensitized with albumin or gamma globulin, the reaction of the intraperitoneal cells including neutrophilic leukocytes could be seen in the center sticks of the bullets mixed with bovine gamma globulin, polyvinyl pyrolidone (P.V.P.) and dextran whose molecular weights were more than 100,000. On the contrary, the cellular reaction could not be seen in the center stick of the bullets mixed with albumin and P.V.P. and dextran whose molecular weights were less than 100,000.As the results of our experiments, it has been clarified that the molecular weight of the foreign body with antigenecity has a tremendous influence on the reaction of the peritoneal cells, and that the so ‐ called cell bound antibody does not cause the reaction by i

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1973.tb00772.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

With the results obtained with our experimental procedures, agar hemolytic plaque formation was demonstrated in the cultured omentum cells of both non‐sensitized and sensitized guinea pigs, administrated sheep red cells in vitro.This experiment provides evidence for antibody formation in the cultured omentum cell

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1973.tb00773.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

The morphological changes of the capillary endothelium in lung have been studied with administration of soluble immune complexes.With the administration of soluble immune complexes, there were noted vacuolarisation, lift‐up phenomenon, arcade formation and subendo‐thelial edema of pulmonary capillar

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1973.tb00774.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

Five autopsy cases of thrombotic microangiopathy, including 3 cases associated with acute promyelocytic leukaemia, were examined macroscopically, light‐and electronmicroscopically.The so‐called hyaline thrombi In thrombotic microangiopathy were composed of fibrin and its degenerative products. Thrombocytes and other blood cells were not seen in the thrombi.At the site of the formation of a thrombus, there was no conspicuous change in the walls of the capillaries and arterioles. It was considered, therefore, that the intravascular deposition of fibrin was the primary event in the development of thrombotic microangiopathy.In regard to the distribution and morphologic findings, there was no basic difference between the microthrombi in cases associated with acute promyelocytic leukaemia and those without it.The bone marrow and some other organs in cases of thrombotic microangiopathy associated with acute promyelocytic leukaemia macroscopically revealed a green colour. Many thrombi composed of leukaemic cells and fibrin were found in the pulmonary arteries of these cases. Furthermore, prominent erythrophagocytosis in the bone marrow and lymph nodes was a common finding in these ca

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1973.tb00775.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

Leukocytosis promoting factor (LPF) of Bordetella pertussis and pertussis vaccine (PV) were introduced into C3H/He mice respectively and the kinetics of colony forming cells (CFC) were assayed by modification of Till and McCulloch's method. Uptake of59Fe and3H‐thymidine into hematopoietic tissues was also examined with similarly treated mice.In vitroincubation of bone marrow cells with LPF did not cause any particular changes on the development of spleen colonies. Increase of CFC in peripheral blood was significant in both LPF and PV treated animals.In the PV treated mice, erythropoiesis in the spleen and granulopoiesis in the bone marrow were remarkably accelerated on the 5th or 7th day of the injection. The number of CFC in the spleen showed a significant increase, which may result from stimulative effect of bacterial endotoxin. On the other hand, CFC of the spleen and bone marrow were unchanged or slightly decreased in number by administration of LPF, although a temporary stimulation of hematopoiesis was confirmed on the 2nd or 5th day of the injection. The functional derangement of endothelial cells induced by LPF is considered to be the dominant cause of increased CFC in the peripheral blood. The involvement of plural regulatory systems of hematopoiesis has been postulated, based upon the nresent exnerimental result

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1973.tb00776.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

Tumors in rats were induced in the urinary system of rats with basic lead acetate (BLA), N‐nitrosodimethylamine (DMN), N‐butyl‐N‐(4‐hydroxybutyl) nitrosamine (BBN), N‐nitrosodibutylamine (DBN), 3‐methylcholanthrene (3‐MC) and 4‐nitroquinollne 1‐oxide (4NQO). These kidney, ureter and urinary bladder tumors were studied by histopatho‐logy, histochemistry, autoradiography and electron microscopy.The tumors of the kidney were classified into the following 4 types; renal cell tumors, embryonal cell tumors, transitional cell tumors and hemangioendotheliomas. However, the tumors of all other regions were of one type, transitional cell tumors. Studies by light and electron microscopy and autoradiography and histochemlcal studies on the histogenesis of tumors of the urinary system in rats showed that renal cell tumors may originate from the renal tubular epithelium and from a part of the metanephrogenic tissue, embryonal cell tumors originate from metanephrogenic tissue and hemangioendotheliomas may originate from blood capillaries. Transitional cell tumors of the kidney, renal pelvis, ureter and bladder originate from tr

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1973.tb00777.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

Age variation of mouse serum alkaline phosphatase activity was ascertained with 263 CBAxC57BL/6 F1, hybrid mice 29 to 366 days old, by the Kind and King method. The values were 10.0 to 22.0, 5.2 to 7.9 and 4.0 to 5.1 K.A. units at 30, 80 to 200 and over 200 days of age, respectively. The mean values and their variation ranges gradually declined with age. The activity in females was constantly higher than in males.Serum alkaline phosphatase activity of 107 Gardner's osteosarcoma‐bearing mice was determined. At 10 to 15 days after transplantation when the grafted tumors were smaller than 3times3 mm, the values were all less than 10.0 K.A. units of those of normal mice. After the tumors had grown to over 3times3 mm in size, the values rose to the extent of 500.0 K.A. units with the increase of tumor size. The increased serum alkaline phosphatase activity was regarded as a marker of tumor growt

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1973.tb00778.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY