摘要:

Morphological changes in the spleens of patients with idiopathic portal hypertension (IPH) were studied and compared with the normal spleen. The study used (1) light microscopy with histometry, (2) scanning electron microscopy (SEM) of the splenic tissue with histometry and (3) SEM of the spleen vascular replica. Histometrical studies by light microscopy showed that the volume of red pulp of IPH was increased in a unit area and to a total of 12 times the normal in the whole spleen. The white pulp was scanty of lymphocytes and decreased in a unit area but it was increased in the whole spleen. SEM of the white pulp of IPH demonstrated many channels formed by reticulum cells and running parallel with each other along the central artery. This finding presumably corresponds to periarterial fibrosis in light microscopy. SEM histometry demonstrated that the venous sinuses of IPH were small but increased in number and occupied the same percentage area in a unit red pulp area as in the normal spleen. The Billroth cord of IPH was narrowed and occupied by thickened reticulum cells, which may cause increased pooling and destruction of blood cells in the enlarged spleen (hypersplenism). SEM of the tissue and vascular replica demonstrated open arterial termination in the Billroth cord in the spleen of IPH as well as in the normal spleen. Venous sinuses in the replica of IPH ran parallel with each other forming bundles with fewer intercommunications than normal.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1979.tb01288.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

The authors have studied the endothelial changes in the aorta and the arteries of heart, kidney and other organs in cholesterol fed rabbits and endotoxin administrated rats through electron histochemical and vascular clearance methods. The vascular changes are variable presumably due to the functional and morphological difference of vessels. The administration of cholesterol and endotoxin may result in increased vascular permeability which is directly related to the qualitative increase and decrease of hydrolytic enzymes in the vascular wall. The permeability of high molecular substances was enhanced with the widening of the intercellular space in contraction of endothelium and the changing of molecular structure of plasma membrane accompanied with plasma lipid, platelets and hemodynamic stress. This fact was confirmed by use of horseradish peroxidase and dextran fesin as tracers of different molecular sizes. The authors emphasize that direct injury and subsequent abnormal contraction of the endothelial cell may initiate increased permeability of plasma protein and lipid in the medial layer and eventually may develop and enhance arteriosclerosis.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1979.tb01289.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

In a previous paper the authors described the morphologic observations that the concomitant administration of ACTH and flavin‐adenine dinucleotide (FAD) to hypophysectomized rats exerted a more potent preventive effect on atrophy of the adrenal cortex of the animals than the single administration of ACTH. The present study was made to electron‐microscopically observe the effect of concomitant administration of ACTH and FAD on atrophy of the adrenal cortex induced with the administration of dexamethasone (Dx). The zona fasciculata of the adrenal gland of rats treated with Dx+ACTH+FAD was morphologically closer in cell organelles such as smooth‐surfaced endoplasmic reticulum, mitochondria and chylomicrons to that of control animals than the counterpart of animals treated with Dx+ACTH only. The zona fasciculata of the adrenal cortex of animals treated with Dx+FAD was morphologically similar to that of animals treated with Dx only. These findings suggested that FAD would potentiate the adrenocorticotropic action of ACTH through its physiologic a

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1979.tb01290.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

A new cultured cell line (HuOS) was established from tumor cells obtained from the pulmonary metastatic foci of a patient diagnosed as chondroblastic osteosarcoma. The tumor cell line was maintained for over 19 months, and morphological and biological characteristics were studied. These cells retained their malignant properties and produced nodules when transferred intramuscularly to nude mice. Morphologically, these nodules revealed a chondromatous pattern.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1979.tb01291.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

A new method to quantitatively evaluate pulmonary arterial change in pulmonary hypertension is presented in this paper. Hypertensive pulmonary arterial changes are classified into four grades according to severity and a score from 1 to 4 is given to each arterial change according to the grading. An index of pulmonary vascular disease is then defined as a relative mean value of all scores. It is concluded that this index accurately expresses the grade of pulmonary arterial change in the whole pulmonary arterial system in any given case and makes possible a detailed statistical analysis of large numbers of cases.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1979.tb01292.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Ten cases of cholecystitis with severe lymphocytic reactions were selected among serially examined 131 cases of cholecystitis or/and cholelithiasis. Gram‐negative bacterial infection, especially E. coli and K. pneumonia seemed to be related as the cause of these severe lymphocytic reactions, but the gallstone revealed no definite influence. We would like to separate the cases with severe lymphocytic reactions as a lymph follicular cholecystitis and consider the possibility of a gram‐negative bacterial infection in b

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1979.tb01293.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

A case of the syndrome of sea‐blue histiocyte is presented in a 53‐year‐old Japanese woman, which is the first recorded case in Japan. The patient had hepatosplenomegaly, bleeding manifestations, mild thrombocytopenia, fatty metamorphosis and cirrhosis of the liver, as well as abnormal serum lipid profiles. Her parents were consanguineous and her maternal grandmother with hepatomegaly died of hepatic failure. Histologically, peculiar histiocytes containing numerous, intracytoplasmic sea‐blue stained granules on May‐Giemsa stain were demonstrated in biopsy materials of the bone marrow, lymph node and liver. The sea‐blue granules in these histiocytes proved to have histochemical staining characteristics of lipogenic ceroid‐like pigment. Ultrastructurally, these granules showed membrane‐bound, pleomorphic inclusions of heterogeneous nature, including electron‐dense amorphous or variegatedly osmiophilic, frequently laminated materials. Enzyme cyto‐chemically, localization of acid phosphatase activity was demonstrated in and around the intracytoplasmic inclusions. With regard to the pathogenesis of the sea‐blue histiocytes in this case, it may be suggested that the existence of the abnormality in lipid metabolism plays an imporant role in intralysosomal ceroidogenesis

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1979.tb01294.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

The case presented here is a three‐month‐old male infant with the Beck‐with‐Wiedemann's syndrome. Clinically, the patient was characterized by macroglossia, visceromegaly, umbilical hernia, microcephalus and other multiple malformations such as facial flame nevus or ear lobe grooves. The patient died of bronchopneumonia at the age of three months, and an autopsy was performed. Morphological examination revealed adrenal cytomegaly, hyperplasia and hypertrophy of the pancreatic islets, adrenal rest tissue in the right testis or hypertrophy of muscle fibers of the tongue associated with fibrous degenerative change, in addition to bronchopneumonia of the lung, causing his death. On electron microscopical examination, the cytomegalic cell of the adrenal was characteristic of large pleomorphic nucleus and granular substances with high density in the cytoplasm. In this case, thorough histologic search revealed no evidence of tumorous growth in the organs, though the exomphalos‐macroglossia‐gigantism syndrome has been of interest in its relationship to the occasional occurrence of Wilms tumor, adrenal carcinoma or o

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1979.tb01295.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Malignant teratoma of the thyroid gland was found in a 19‐year‐old female. She was given a combined surgical and irradiation treatment. The tumor was 5.0·4.0·3.6 cm and located in the lower right lobe of the thyroid gland. Light and electron microscopical examinations revealed mature or immature derivatives of the three primordial germ layers. The dominant portion of the tumor was composed of numerous masses of cancerous epithelial cells, which were distributed randomly in the mesenchymal tissue. Tumor cells invaded and replaced the surrounding thyroid tissue with occasional tumor thrombi in the lumens of blood vessels. No metastatic tumor was found in the lymph nodes. The patient was given a course of external irradiation, a total dose of 4000 rads, to the right thyroid area. Eight months after surgery, she is well and without any evidence of recurrence or metas

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1979.tb01296.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Four cases of anaplastic carcinoma of the thyroid, composed of one small cell carcinoma and three giant cell carcinomas, were studied with electron microscope. In the case of small cell carcinoma, fine cytoplasmic interdigitations and junctional complex between apposing cytoplasmic membranes of neighbouring tumor cells and a few microlumina within tumor cell clusters surrounded by well‐defined basal lamina were seen. In the cases of giant cell carcinoma, occasional cytoplasmic interdigitations as well as desmosomal structures were detected even in tumor cells markedly pleomorphic and anaplastic. Abundant cytoplasmic organelles including profiles of Golgi apparatus, rough endoplasmic reticulum and a few mitochondria were seen in the cytoplasm of tumor cell of all four cases. Of interest to note was that all giant cell carcinomas demonstrated evidences of fairly well differentiated tumor within anaplastic carcinoma, indicating probable pre‐existing either benign or malignant epithelial neoplasm more differentiated, with its subsequent anaplastic trasformation. Findings in the present study support an assumption that these anaplastic tumors are derived from the follicular epithelium of the thyroid gland. In addition, it can be said that tumor cells of the small cell carcinoma provide evidences suggesting functional differentiation of carcinoma cells to a certain extent, yet unable to produce thyroglobu

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1979.tb01297.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY