ISSN:0737-1454    

DOI:10.1002/stem.5530010102

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

摘要:

AbstractSixteen histologically documented testicular cancer specimens obtained at diagnostic procedures following induction chemotherapy with cis‐plati‐num containing regimens were cloned in soft agar. Seven (44%) of the specimens cultured formed colonies with a mean cloning efficiency of .021%. Colony formation was observed with all the common histologic subtypes of testicular cancer (seminoma, embryonal carcinoma, choriocarcinoma and mixed tumors).In vitro drug sensitivity tests were performed using cis‐platinum, vinblastine and VP‐16. Three of four specimens demonstrated a decrease in colony formation to less than 50% of controls after a 1 h exposure to VP‐16 at 300 μg/ml. Two of these patients had a response to treatment with a VP‐16 based salvage regimen.Immunoperoxidase staining of the colonies for alpha feto protein and human chorionic gonadotropin were correlated with the serum levels of these tumor markers determined at the time the specimen was obtained. In three instances the same markers were elevated in the serum as detected within cells which formed the colonies; however, in two other cases the marker(s) that was elevated in the serum was not expressed in the colonies. In one case a biopsy of a residual retro‐peritoneal mass following chemotherapy histologically was a teratoma, but it formed colonies in the assay which stained positive for alpha feto protein. This patient subsequently developed an elevated serum alpha feto protein.These studies have demonstrated that (a) testicular cancer can be cloned directly in soft agar; (b) a heterogeneous tumor cell population exists in meta‐static testicular cancer specimens; and (c) a dose response exists for VP‐16 in relapsed testicular cancer which suggests that increasing the dose of VP‐16 may be c

ISSN:0737-1454    

DOI:10.1002/stem.5530010103

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

摘要:

AbstractIn order to study the pathogenesis of primary acquired sideroblastic anemia (PASA), bone marrow and/or peripheral blood specimens obtained from four patients with PASA were cultured for erythroid colony‐forming units (CFU‐E), erythroid burst‐forming units (BFU‐E), and granulocyte/macrophage colony‐forming units (GM‐CFU). The number of CFU‐E was markedly decreased in all four patients. CFU‐E colonies consisted exclusively of normal‐appearing erythroblasts, while ringed sideroblasts were observed only in scattered single erythroblasts or in small erythroblast aggregates. In one case, very few BFU‐E colonies containing both normal‐appearing erythroblasts and ringed sideroblasts were detected. In addition, the number of GM‐CFU was significantly decreased in three out of the four cases. These findings may suggest that there are abnormalities in the pluripotent hemopoietic stem cells at leas

ISSN:0737-1454    

DOI:10.1002/stem.5530010104

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

摘要:

AbstractDacarbazine (DTIC) is an agent with clinical activity against human malignant melanoma. We have explored two methods for activating DTIC so it may be used in vitro in a human tumor cloning system. The activation of DTIC by white light was found to be a viable alternative to utilizing a microsome plus cofactor system for bioactivation. The microsome plus cofactor system itself actually caused some inhibition of tumor colony formation. Light activation of DTIC appears to be a reliable and simple method which allows testing of DTIC in an in vitro soft agar culture system.

ISSN:0737-1454    

DOI:10.1002/stem.5530010105

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

摘要:

AbstractA murine monoclonal antibody (anti‐MY7) has been developed that detects an antigen expressed by 6% of normal human bone marrow cells, including approximately 40% of myeloid colony‐forming cells (CFU‐C). The number of bone marrow cells and CFU‐C expressing MY7 is significantly increased in regenerating bone marrow, but less than 5% of peripheral blood CFU‐C express the MY7 antigen. Erythroid precursors are MY7 negative from peripheral blood and bone marrow. Thymidine suicide studies indicate that CFU‐C in S‐phase tend to be MY7 positive while CFU‐C not in S‐phase are MY7 negative. MY7 expression thus appears to identify a fraction of CFU‐C that is act

ISSN:0737-1454    

DOI:10.1002/stem.5530010106

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

摘要:

AbstractMouse bone marrow, obtained from donors three days after treatment with 5‐fluorouracil, had a very low ability to form macroscopic spleen colonies in irradiated mice at 10 days after transplantation of the cells (CFU‐S10); such marrow also had no detectable erythropoiesis repopulating ability but did have near normal marrow repopulating ability and spleen megakaryocyte repopulating ability.Incubation of this marrow in vitro for 7 days with medium containing growth factor preparations (a) pregnant mouse uterus extract plus human spleen conditioned medium or (b) mouse spleen conditioned medium, resulted in marked increases in CFU‐S10and in cells with erythropoietic repopulating ability together with maintenance of cells with marrow repopulating ability. These responses were not observed in cultures with control medium alone. Spleen megakaryocyte repopulating ability was also maintained in the presence of the factor prepara

ISSN:0737-1454    

DOI:10.1002/stem.5530010107

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

ISSN:0737-1454    

DOI:10.1002/stem.5530010108

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

ISSN:0737-1454    

DOI:10.1002/stem.5530010109

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

ISSN:0737-1454    

DOI:10.1002/stem.5530010110

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

ISSN:0737-1454    

DOI:10.1002/stem.5530010101

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY