ISSN:0737-1454    

DOI:10.1002/stem.5530020102

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractTumor chemosensitivity assays require the frequent in vitro use of antineoplastic drugs. Economy and convenience are greatly enhanced if these drugs are stored in aliquots for use as needed. This study investigated the stability of eight common antineoplastic agents at — 60°C. Activity was measured as inhibition of colony formation in a commonly used soft agar culture system using an experimentally induced murine sarcoma. Our results indicate no loss of activity with these drugs under the specified storage conditio

ISSN:0737-1454    

DOI:10.1002/stem.5530020103

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractSubpopulations of granulosa cells of differing responsiveness to epidermal growth factor (EGF), fibroblast growth factor (FGF), pituitary gonadotropin preparations, and rat erythrocyte suspensions (RBC) have been detected using an anchorage‐independent clonal agar assay. All growth factor preparations were capable of enhancing colony formation when added alone, and elicited cloning efficiencies as high as 35% when added to the culture system at optimal concentrations in a variety of combinations. The FGF preparation was the single most effective stimulator of colony formation, augmenting both colony numbers and colony size at concentrations as low as 50 ng/ml. However, unlike the other growth factors in this assay system, a plateau in responsiveness could not be reached even at levels as high as 1 μg/ml. NIH‐FSH‐P2 and NIAMMD‐bLH‐4 were considerably less potent than other growth factors. Both preparations were inactive at concentrations less than 1 μg/ml and produced an optimal response

ISSN:0737-1454    

DOI:10.1002/stem.5530020104

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractMedia conditioned by fibroblast‐like cells derived from organs active in fetal lymphohematopoiesis were studied for their effects on adult gran‐ulocyte/macrophage colony‐forming units (CFU‐GM). Fibroblasts from fetal liver produced a factor stimulatory for CFU‐GM, whereas fibroblasts from fetal marrow produced a factor inhibitory for CFU‐GM which was not completely relieved by adding indomethacin to the assay. Our studies indicated that neither fetal marrow nor fetal liver produced factors affecting lymphocyte colony‐forming units (CFU‐L). Cell‐cell interactions between fibroblast‐like cells derived from fetal liver or marrow and normal adult CFU‐GM were also studied. We observed that fibroblasts derived from both fetal and adult marrow inhibited colony formation, whereas inhibition in the presence of fetal liver fibroblasts was minimal. Loss of inhibitory activity by a liver fibroblast cell line over repeated passages was seen. Differential analysis of colonies formed above an adherent layer of fetal marrow fibroblasts suggested that these fibroblasts suppress myeloid/macrophage differentiation to a far greater degree than did adult marrow fibroblasts. A role in the regulation of fetal lymphohematopoiesis may be played b

ISSN:0737-1454    

DOI:10.1002/stem.5530020105

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractT‐cell colonies derived from peripheral blood mononuclear cells plated in agar wtih phytohemagglutinin (PHA) contain about 5‐7% E‐, OKT3‐, OKT6‐, TdT‐, SIg‐cells phenotypically similar to bone marrow precursors since they are also RFBl+, OKTIO+, HLA‐DR+, and PNA+. These cells can generate secondary E+, OKT3+T‐cell colonies when replated in the presence of both PHA and a colony‐promoting activity distinct from the T‐cell growth factor, Interleukin 2. These observations suggest that E‐, OKT3‐cells detected in primary PHA‐induced T‐cell colonies belong to a pool of prethymic cells which are able to mature into T‐lymphocytes of T4 and T8 type in the absence

ISSN:0737-1454    

DOI:10.1002/stem.5530020106

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractThe First International Workshop on T‐cell colonies in semisolid media has released some important applications of the method: (a) it should be a very useful tool for the study of the different steps of T‐cell differentiation and their control mechanisms; (b) it may provide methods for the induction and initial cloning of functional subsets of mature T cells before subsequent expansion in liquid culture; (c) such cloned T cells may be used not only as a source of functional cells and lymphokines, but also to screen otherwise undetected subgroups of the major histo‐compatibility complex as well, hopefully for immunotherapy of malignancies and autoimmune or infectious diseases; (d) the study of lymphocyte colony formation should be complementary to investigations dealing with other lineage precursors (CFU‐GM, BFU‐E, CFU‐E, CFU‐Meg) for the monitoring of hemopoiesis in a variety of immunohematologi

ISSN:0737-1454    

DOI:10.1002/stem.5530020107

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

ISSN:0737-1454    

DOI:10.1002/stem.5530020108

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

ISSN:0737-1454    

DOI:10.1002/stem.5530020101

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY