摘要:

AbstractA method of extracting texture information from a 3‐D grey level distribution is proposed, based on the concept of accessibility. We apply this method to cell nuclei in confocally scanned images. Inaccessibility of a point is defined as the minimal sum of optical density that is encountered when traversing from outside the object of interest to this point. The inaccessibility of all object voxels can be determined by applying a Grey weighted Distance Transform (GDT). From the inaccessibility patterns three texture features are extracted: object inaccessibility, object inaccessibility compared to a homogeneous distribution, and object homogeneity. Normalization of these features enables objective inter object comparison of texture. Simulations on artificial textures in varying object shapes show the shape independence and discriminating power of the proposed texture features. The processing steps that are necessary for confocally scanned data are discussed and an application of analysing a cell nucleus is show

ISSN:0966-9051    

DOI:10.1002/1361-6374(199403)2:1<1::AID-BIO1>3.0.CO;2-I

出版商:IOP Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractWe have characterized the highly detailed internal features of acetylcholine receptor (AChR) clusters on cultured rat myotubes by applying a novel image analysis technique—spatial fluorescence autocorrelation. AChR clusters were observed with total internal reflection fluorescence (TIRF) under high magnification after labelling the AChRs with tetramethylrhodamine α‐bungarotoxin, and recorded as CCD images. AChR clusters (on the tens of microns size scale) appear to be composed of microclusters (on the tenths of micron size scale), and the microclusters are sometimes organized into paracrystalline arrays. These structural features may reflect those of a protein meshwork that may underlie each cluster. To characterize these features, we have applied a spatial fluorescence autocorrelation technique which allows us to extract six parameters from each cluster image: average size of microclusters, contrast of microclusters, average spatial period of the paracrystalline arrays, contrast of periodicity, average fluorescence intensity, and average anisotropy of microclusters. These parameters were then followed as a function of myotube age after plating, and upon treatments with drugs which previously have been shown to gradually eliminate clusters. The drugs used were oligomycin (an energy metabolism inhibitor) and carbachol (a nicotinic AChR agonist). We show that myotube age only slightly affects the image parameters based on the spatial autocorrelation. Oligomycin induced significant decreases in the size of microclusters, contrast of microclusters, spatial period, contrast of periodicity, and anisotropy, whereas the carbachol study showed significant increases in the size of the microclusters and the anisotropy of the microclusters. These results suggest that these two drugs may act through different mechanisms in eliminating clus

ISSN:0966-9051    

DOI:10.1002/1361-6374(199403)2:1<22::AID-BIO2>3.0.CO;2-3

出版商:IOP Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractWe consider the form of the interference term image in scanning interference microscopes employing finite sized coherent and incoherent detectors. We show, inter alia, that for the case of incoherent photodetectors the interference term image in scanning confocal and scanning conventional systems behave identically in all respects including optical sectioning.

ISSN:0966-9051    

DOI:10.1002/1361-6374(199403)2:1<36::AID-BIO3>3.0.CO;2-O

出版商:IOP Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractNew simple and fast methods for 3‐D depth perception and interaction in volume visualization and quantitative analysis of dual‐fluorescence confocal microscopy images are presented. By integrating a 3‐D display and an associated 3‐D input device, a system has been developed which creates an autostereoscopic confocal microscopy workstation environment on an IBM‐compatible personal computer. The system incorporates appropriate 3‐D image processing algorithms and enables the user to interactively address subregions in the data volume to identify domains of interest for subsequent volume and distance measurements. In the analysis of chromosomes in interphase nuclei these capabilities are shown to be useful in finding distances between chromosome domains and their respect

ISSN:0966-9051    

DOI:10.1002/1361-6374(199403)2:1<41::AID-BIO4>3.0.CO;2-Z

出版商:IOP Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractComputer motion analysis was tested as an ultra‐sensitive imaging technique to detect minute displacement of corn seedling growth. The motion analysis software displayed motion in vector form, i.e. as both magnitude and direction of seedling elongation. The system was able to detect increments of growth in a non‐intrusive, non‐contact fashion as minuscule as 5 mi

ISSN:0966-9051    

DOI:10.1002/1361-6374(199403)2:1<57::AID-BIO5>3.0.CO;2-D

出版商:IOP Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractWe present a practical solution to the problem of reliably relocating a cell or similar microscopic object, in the presence of inaccurate stage mechanics. Our technique involves storing the relative coordinates of a ‘constellation’ of nearby slide objects, which is then used in a Bayesian statistical model to select the most likely object upon subsequent relocation. This approach has the significant benefit of assigning honest posterior probabilities, so enabling uncertain relocations to be identified. Results are presented for a cervical cytology application. Experimentally derived calibration curves are given to argue the statistical reliability of the mo

ISSN:0966-9051    

DOI:10.1002/1361-6374(199403)2:1<62::AID-BIO6>3.0.CO;2-O

出版商:IOP Publishing Ltd    

年代:1994

数据来源: WILEY