摘要:

Cowpox virus and vaccinia virus WR specific DNases were identified by an in situ assay using DNA-containing acrylamide gels. A DNase present in the isolated virions of strain cowpox and vaccinia WR was identified. Its properties as determined by the in situ assay resembled those of the well-characterized DNase Vi. The assay was also used to follow the time course of ‘acid’ DNase induction by cowpox virus in primary chick embryo fibrobla

ISSN:0300-5526    

DOI:10.1159/000148915

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

The protein patterns of monosomes and polysomes isolated from the T-22 line of SV40-transformed GMK cells and from uninfected CV-1 cells and CV-1 cells infected with SV40, adenovirus 5, or vesicular stomatitis virus were analyzed by two-dimensional PAGE. All gel patterns were similar except for the presence of one additional protein associated with T-22 monosomes.

ISSN:0300-5526    

DOI:10.1159/000148916

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

Ratios of the sedimentation coefficients for alfalfa mosaic virus components are shown to be independent of the virus concentration and the density of the solvent. Different numbers of components are observed in solvents of different density. This implies that in sedimentation velocity experiments an estimate of the number of components of a multicomponent virus should involve centrifugation in solvents of different density. For some viruses, estimates of the sedimentation coefficients of individual components can be obtained from the coefficient ratios observed in unfractionated solutions and the sedimentation coefficient of the most easily purified component.

ISSN:0300-5526    

DOI:10.1159/000148917

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

Conditions necessary for the establishment and maintenance of transformation of human cells by wild type and temperature-sensitive mutants of SV40 were examined. For both early and late mutants, the frequency of transformation was found to be up to 5-fold higher, and virus yield 100-fold lower, at 39° than at 33°. No such effect was observed with the wild type virus under the same conditions. This observation is apparently at variance with previously published work, but may be explained by the semi-permissive nature of the cells that we used. Increasing the temperature to 40.5° caused cells transformed by the early mutant, tsA30, to lose T-antigen as detectable by staining, and also to lose the abilit to grow to high density, while it produced no effect on cells transformed by wild type vir

ISSN:0300-5526    

DOI:10.1159/000148918

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

Cultures of human cells transformed by SV40 were found to release infectious virus, even after several passages in vitro. Virus shedding by these cultures did not depend on propagation of virus from cell to cell, as it was not affected by anti-SV40 antiserum that could effectively block virus propagation in acutely infected cells. Cells transformed by the ‘early’ temperature-sensitive mutant tsA30, and maintained at the restrictive temperature of 39°, shed virus in reduced amount. Finally, xeroderma pigmentosum cells transformed by SV40 were also found to release virus, indicating that the enzymes of excision and repair of UV-induced damage to DNA probably were not involved in the molecular mechanism underlying virus shed

ISSN:0300-5526    

DOI:10.1159/000148919

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

Several factors affected the interferon sensitivity of human cytomegalovirus in human foreskin fibroblast cultures. An inoculum of infected cells was up to 300-fold less sensitive than a cell-free inoculum of equivalent input multiplicity. A 10-fold increase in the dose of infectious units of either type of inoculum was associated with a 10-fold or greater decrease in interferon sensitivity. Several aspects of the virus-cell interaction were examined and parameters indicative of cell infection were less inhibited by interferon than were those for virus replication.

ISSN:0300-5526    

DOI:10.1159/000148920

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

Organic compounds in cell culture harvests known as membrane-coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy-fiberglass filters. Subsequent elution with high pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100-fold.

ISSN:0300-5526    

DOI:10.1159/000148921

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

Viruses isolated from patients with hand, foot and mouth disease in widespread outbreaks in Japan in 1973 were identified as enterovirus 71. Although cases with aseptic meningitis were observed concurrently, the main clinical symptom associated with enterovirus 71 infection was hand, foot and mouth disease.

ISSN:0300-5526    

DOI:10.1159/000148922

出版商:S. Karger AG    

年代:1978

数据来源: Karger

ISSN:0300-5526    

DOI:10.1159/000148923

出版商:S. Karger AG    

年代:1978

数据来源: Karger