摘要:

Red blood cells freeze‐preserved with 40% W/V glycerol in an ionic or low ionic medium at −80C were washed in one of four commercially available systems: the Haemonetics Blood Processor 15 using a continuous‐flow centrifugation procedure, the Fenwal Elutramatic using a continuous‐flow centrifugation procedure, the IBM Blood Processor using an automated serial centrifugation procedure, and the Huggins Cytoglomerator using a dilution/agglomeration procedure. Determinations of red blood cell recovery and leukocyte and platelet removal were made for each of these groups. The dilution/agglomeration procedure produced lower red blood cell recovery values and poorer leukocyte and platelet removal than did any of the three wash systems using sodium chloride solutions. The values obtained with the systems using sodium chloride solutions were slightly but significantly di

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17177128877.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

Each of 15 healthy male volunteers was treated with 650 mg of aspirin 24 hours before the autologous transfusion of one unit of freeze‐preserved platelets. Freeze‐thaw‐wash recovery valuesin vitro, viability and functionin vivo, and the bleeding time and platelet aggregation response were measured. The platelets were frozen with 4 or 5 per cent dimethylsulfoxide (DMSO) at an overall rate of 2 to 3 C per minute and were stored at −80 C in a mechanical freezer for up to eight months. They were washed by dilution/centrifugation.The mean recoveryin vitroof platelets frozen with 4 per cent DMSO was 76 ± 16 per cent; the value was 64 ± 16 per cent for platelets frozen with 5 per cent DMSO. The mean in vivo51Cr recovery of autologous platelets frozen with 4 per cent DMSO was 34 ± 6 per cent, and for platelets frozen with S per cent DMSO it was 33 ± 7 per cent. In both groups the platelet lifespan was normal. There was a significant reduction in bleeding time after the transfusion of a single unit of autologous platelets preserved with either 4 or 5 per cent DMSO, but no improvement in the aspirin‐induced platelet aggreg

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17177128894.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

The present report describes a method for125I anti‐IgG antiglobulin testing. The method involves the use of a labeled DEAE cellulose IgG fraction of goat anti‐human IgG globulin. Leukocyte‐free red blood cells were used in the test procedure. Both the labeled antiserum and the red blood cell suspensions were diluted in normal rabbit serum to prevent nonspecific uptake of labeled antiserum by normal, un‐coated red blood cells.The results of the study show that the method is highly reproducible and relatively easy to perform once the labeled anti‐IgG is prepared. Furthermore, the use of labeled antiserum permits analysis of antiserum specificity by radioimmunoelectrophoresis. The problem of determining anti‐IgG/IgG binding ratios remains the major disadvantage to using the method to determine precise amounts of human IgG coating red

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17177128878.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1977.tb01260.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

A simple and inexpensive method for the freezing of human blood lymphocytes at −80 C is described. Lymphocytes were frozen in either polystyrene tubes or polyolefin bags. Optimal recovery of cells was obtained when 5 per cent DM SO and 62 per cent plasma were used as cryoprotectants. Viable cell recovery, as assessed by response to phytohemagglutinin (PHA) and pokeweed mitogen (PWM), was greater than 80 per cent after 60 days of storage. In addition, percentages of T and B lymphocytes were essentially unchanged. The excellent viable cell recovery in polyolefin bags suggests that it is possible to cryopreserve large numbers of lymphocytes in a single containe

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17177128879.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

A patient with acute leukemia who typed as AintB received 10 packs of platelets of group 0. Subsequent transfusion of A1B blood resulted in a hemolytic transfusion reaction. Anti‐A1was detected in the serum and on the red blood cells of a post‐transfusion sample. This anti‐A1reacted with the transfused A1B red blood cells and other A1cells, but not with the patient's pretransfusion red blood cells. The plasma of the transfused platelet concentrates had a high titer of immune a

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17177128880.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

In patients with severe allergic reactions to plasma proteins it is possible to observe such reactions to even the small quantity of plasma contained in platelet concentrates. A platelet washing solution was designed, and platelet concentrates for four such patients were washed before infusion. Transfusion reactions were completely eliminated by the washing procedure. Platelet recovery was equivalent to that of unwashed platelets, and hemostatic effectiveness of the infused platelet concentrates was evidenced by abrupt cessation of bleeding episodes, including purpura and hematuria. Platelet washing represents a valuable, rapid and simple approach to the problem patient with thrombocytopenia and severe reactions to plasma proteins.

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17177128881.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

The initial uptake of adenine from plasma by human red blood cells was measured at 0,10, and 20C. Initial uptake is completed in several minutes as distribution equilibrium is reached; however, total uptake requires several weeks at 4C. Adenine inside the red blood cell was shown to egress to the plasma if the equilibrium shifted due to plasma dilution or exchange.

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17177128882.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

HBsAg was detected in two commercial clotting factor concentrates: Proplex (Hyland) and Konyne (Cutter). HBsAg was positive by RIA in samples of Proplex but not Konyne. Immune‐electron microscopy, however, demonstrated clumped small spheres of HBsAg in both preparations, and lamellar‐type strands were seen in several preparati

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17177128883.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

The finding that En(a ‐) red blood cells are Wr(a‐b‐), and thus probably represent a “null” phenotype of the Wright system, has provided evidence of the independence of the Wright blood group system from many others. Detection of blood group antigens on “null” red blood cells almost certainly indicates that those antigens do not belong to the same blood group system as the “null” cells. In the case of Wright, lack of certainty that En(a ‐) is the “real,” or only, null type slightly reduces the

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17177128884.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY