摘要:

Guinea pigs were immunized with 2.4 mg of killed tubercle bacilli in adjuvant and Tuberculin Purified Protein Derivative (PPD) was used as test antigen. Peritoneal exudate cell migration inhibition was more marked one year than one month after sensitization. Macrophage cytophilic antibody (detected by rosette formation with antigen coated sheep red cells) was found on the surface of peritoneal macrophages and in sera at one year, but was barely detectable at one month. One year sera as final media in migration chambers inhibited migration of normal peritoneal cells, so did one month sera. Incubation of normal cells in one year sera followed by washing led to migration inhibition. Sera of guinea pigs passively sensitized with the immune sera had haemagglutinating activity against PPD coated sheep red cells. In some of the animals injected with one year sera cytophilic antibody was demonstrable on the surface of peritoneal cells and the migration of these cells was slightly inhibited by antigen. The degree of migration inhibition did not correlate with haemagglutinating and haemolysing antibody titres.

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00123.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

A new method for the evaluation of the phagocytic and bactericidal activities of neutrophil granulocytes in human disease has been developed by which the total number of viable bacteria and the number of viable intracellular bacteria in anin vitrophagocytic system are measured. Standardization of the granulocyte, bacteria and serum opsonin concentrations at their optimum has made available a sensitive method by which minor variations in neutrophil granulocyte functions can be measured. In a preliminary clinical study, patients with unrecognized abnormalities of neutrophil granulocyte functions were readily diagnosed. The results indicate that abnormal neutrophil granulocyte function is more prevalent than realized.

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00124.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Albuminglutamate (Alb. glut.), the salt of albumin and L‐glutamic acid, is formed as a crystalline precipitate when a solution of albumin in half‐saturated ammonium sulphate solution is acidified with L‐glutamic acid lower than the isoelectrical point of albumin, pH 4.7. By acidification down to pH 4.1, the crystallization of albumin as Alb. glut. is quantit

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00125.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

The elimination and tissue distribution of different batches of rabbit antimurine antilymphocyte globulin and antihuman antilymphocyte globulin were studied by means of a paired‐label technique after injection into mice. The identical over‐all clearance rates of antimurine ALG and control rabbit IgG judged by whole‐body count compared with a slightly lower plasma level of ALG indicated an increased binding of the injected́ ALG to tissues. The plasma deficit of ALG was balanced by a higher uptake in the spleen, liver and kidney, but not in the thymus. In particular, the spleen was found to exhibit a much higher avidity for ALG than for normal IgG, while the actual elimination rates for the two globulins were the same for spleen, other tissues and plasma. The interpretation follows that no accumulation of “fixed” ALG occurred within lymphoid organs, which would have been expected if the “blindfolding” theory of the immunosuppressive effect of ALG is correct. This difference in avidity was roughly correlated to the specificity of the ALG pr

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00126.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Haemagglutinin of vesicular stomatitis virus, Indiana serotype, was propagated in BHK21/13S cells, using diluted seed virus and multicyclic growth. In a 15 to 45 per cent sucrose gradient two haemagglutinins with different sedimenting properties could be separated. The proportions of these haemagglutinins depended on the input multiplicity of infection. In preparations obtained with undiluted seed virus, the proportions of the more slowly sedimenting haemagglutinin was strongly increased. The infectivity was associated with the lower band. In electron microscopy virions were found in the lower band whereas the upper band consisted of so‐called T particles and some rosettes. The two haemagglutinating components could also be separated by their densities in a CsCl gradient. The densities were 1.22 g/cm3for the virion‐containing band and 1.18 g/cm3for the upper band. The two haemagglutinating components had the same protein composition in acrylamide gel electrophoresis. The results obtained suggest that, in addition to the virion, the shorter T particles also possess haemagglutinating capac

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00127.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Rat hearts were transplanted across major histocompatibility loci in two donor‐recipient combinations. A pronounced and specific suppression of rejection was obtained when recipients were treated with one injection of bone marrow cells 7 days prior to allografting. Histological signs of immune reactions in the grafts were found and some rats produced circulating alloantibodies. Skin grafts were rejected in the one combination but were accepted in the other, when applied to recipients with well tolerated hearts of the same genotype. Normal response of recipient spleen cells in GVHR was obtained, and parabiotic union between recipient with functioning grafts and normal unmodified rats did not effect function of the transplants. The suppressive effect of marrow pretreatment could not be transferred with serum taken from rats at the time of transplantation. The possible mechanisms are discusse

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00128.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Sera from animals, mainly of the Red Danish Milkbreed, have been examined by immunochemical analyses in order to select possible IgG‐2 immunoglobulin deficiencies. According to previous findings a low frequency was found in the healthy animal material (1 out of 417 animals) whereas the frequency of this immunoglobulin deficiency was strikingly much higher among diseased animals, especially among animals with pyogenic infections (13 out of 93 animals). It was assumed that lack of IgG‐2 may be associated with a reduced resistance to infections, in particular those caused by pyogenic bacte

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00129.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

The host ranges of 113 typing bacteriophages have been studied at their Routine Test Dilution (RTD) for 486 strains ofPseudomonas aeruginosa. The resulting data matrix has been analysed by numerical allocating procedures employed in numerical taxonomy. The Jaccard‐Sneath similarity index (SJS) and the simple matching coefficient (SSM) were calculated and the results of the weighted and the unweighted pair group average cluster analysis (WPGA and UWPGA) compared. The SSMseemed to produce misleading results. Phages that lysed less than 15 per cent of the bacterial strains tended to be grouped together at high similarity levels, regardless of real differences in their host ranges. For such phages, there was a linear relationship between per cent lysis and SSM. The best representation of the similarity index was obtained with the UWPGA applied to the SJS‐matrix ([SJS] UWPGA). This rendered a cophenetic correlation coefficient of 0.9303 in contrast to r = 0.8933 with the [SJS] W

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00130.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

The quantitative technique of the British Standard (BS 3286:1960) has been applied to four phenolic disinfectants. Comparisons showed that regressions made with log‐probit transformations were preferable to log‐log. The British Standard quantitative technique as employed by us has been found to act as a useful adjuvant toKelsey&Sykes(1969) capacity test in the selection of use‐dilutions for disinfec

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00131.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

The minimum performance concentration of five non‐phenolics have been tested by the British Standard Quantitative Technique which is a suspension procedure. By least squares regression in log‐probit and log‐log graphs, the 99.99 per cent kill concentrations of the disinfectants have been calculated. For non‐phenolics, the unweighted log‐probit least square regression has been found preferable to end point deter

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00132.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY