ISSN:0732-6580    

出版商:OVID    

年代:1982

数据来源: OVID

摘要:

Two monoclonal antibodies produced by hybridomas that define cell surface antigens expressed on cultured human melanoma cells were identified by an indirect125-protein A binding assay. These antigens were not detectable on autologous or allogeneic lymphoblastoid cells. The first antibody (an lgG2bimmunoglobulin) bound to 17 of 17 melanoma lines, 12 of 14 carcinomas and sarcomas, 2 of 2 fetal cell lines, 0 of 13 lymphoblastoid cell lines, and weakly to 3 of 7 leukemia lines. The second monoclonal antibody (IgG1) reacted with 13 of 17 melanomas, 3 of 14 other solid tumor cell lines, and weakly with the fetal cells, but not with the leukemic or lymphoblastoid cell lines. Quantitative absorption of these two monoclonal antibodies confirmed that the antigens were expressed on the melanoma cells but were absent from autologous B-cell lines derived from the same cancer patient. Immunoprecipi-tation of lactoperoxidase125I-labeled membrane antigens from the melanoma cells followed by polyacrylamide gel electrophoresis identified a 95K protein complexed with the IgG2bantibody and a 120K protein bound to the IgG1antibody.

ISSN:0732-6580    

出版商:OVID    

年代:1982

数据来源: OVID

ISSN:0732-6580    

出版商:OVID    

年代:1982

数据来源: OVID

摘要:

A weakly immunogenic tumor, sarcoma T241, was implanted sub-cutaneously on one hind foot of syngeneic C57B1/6 recipients. After 3 weeks of tumor growth, feet bearing primary tumors were amputated and 24 h later liposomal immunotherapy was begun. Treatments, which were given twice a week for 2 weeks, consisted of intravenous injections of liposomes containing either medium alone or medium from concanavalin A-stimulated spleen cells containing lymphokines. Two weeks after amputation, mice were killed for evaluation of lung metastases. Thirty-two percent of the animals treated with liposomes containing lymphokines had no metastases, but only 3, 5, and 0% were metastasis-free in the controls receiving either no treatment, liposomes containing medium alone, or lymphokines alone, respectively. The liposome—lymphokine-treated group also showed significantly enhanced survival, compared with the control groups.In vitrostudies showed significant tumoricidal activity of peritoneal macrophages from liposome-lymphokine-treated animals against T241 cells and B16 melanoma cells, compared with macrophages from control groups. These studies confirm and further extend previous observations by Fidler (1) to yet another malignant tumor model, and thus provide a rational basis for exploration of similar therapeutic approaches to those human cancers with predilection for lung and, perhaps, liver metastases.

ISSN:0732-6580    

出版商:OVID    

年代:1982

数据来源: OVID

摘要:

Thymosin preparations show great promise as modulators of immune function, and such modulation may be of benefit to immunosuppressed cancer patients. A phase I trial was conducted with the agents Thymosin Fraction 5 (TF-5) and Thymosin α1 (Tα-1). Twenty-two patients with advanced malignancy received a single intramuscular injection of either TF-5 or Tα-1 according to a predetermined dose escalation scheme. TF-5 was generally well tolerated in single doses from 60 mg/m2to 480 mg/m2. Fever occurred in 3 of 3 patients at the 960-mg/m2dose. Limiting toxicity for this preparation was pain at the multiple injection sites required to administer the large volumes of material at 960 mg/m2. Tα-1 was tolerated without toxicity over the dose range of 600 μg/m2—9.6 mg/m2. The optimum dose chosen for phase II trials will depend on the biological modification of the immune system induced by these agents rather than on toxicity.

ISSN:0732-6580    

出版商:OVID    

年代:1982

数据来源: OVID

摘要:

We examined the activation to the tumoricidal state of normal F344 rat and C57BL/6 mouse alveolar macrophages (AM) by liposomes containing entrapped hydrophilic muramyl dipeptide (H-MDP) or a lipophilic muramyl dipeptide derivative (MTP-PE) inserted directly into the liposome membranes. The superiority of liposomes containing the lipophilic MTP-PE over liposomes containing H-MDP forin vitroand/orin vivoactivation of AM was demonstrated in several experiments. First, the i.v. injection of liposomes containing a dose of MTP-PE equal to H-MDP led to higher levels of AM-mediated cytotoxicity as measured byin vitroassay. Second, AM harvested from mice given i.v. injections of liposomes containing MTP-PE maintained their tumoricidal activity for a longer period (5 days) than AM harvested from mice given i.v. injections of liposomes containing H-MDP (3 days). Similar results were obtained from experiments of AM activationin vitro. These data demonstrate that, for thein situactivation of AM, liposomes containing a lipophilic derivative of muramyl dipeptide are superior to liposomes containing hydrophilic muramyl dipeptide.

ISSN:0732-6580    

出版商:OVID    

年代:1982

数据来源: OVID

摘要:

Fifty-three patients who had metastatic malignant melanoma received active specific intralymphatic immunotherapy (ASILI) using cultured allogeneic melanoma cells to evaluate clinical response. Six patients entered the study after surgical excision of their metastases (Group I). Three have had recurrences and three remain free of disease. Thirteen patients with disease that could be evaluated received prior chemotherapy and none responded (Group II). Thirty-four patients received ASILI as the primary treatment for distant metastatic disease (Group III). Nine responses were noted (26%), two complete and seven partial. Two additional patients were stable. The median survival of those who responded or were stable was 19 months, while the median survival for the remaining 23 patients was 6 months. This difference is statistically significant (p < 0.04). This is the first report of clinical responses to ASILI using cultured allogeneic cells, making this technique, which previously required tumor cell suspensions prepared from surgical specimens and frozen for later use, feasible for more general application.

ISSN:0732-6580    

出版商:OVID    

年代:1982

数据来源: OVID

摘要:

Thiabendazole (TBZ), when given alone, stimulates hyperplasia of subcapsular thymocytes and of germinal center cells in normal mice. When given with the thymus-dependent neoantigen dinitrofluorobenzene, TBZ stimulates all compartments of the lymphohemopoietic system, including pluripo-tent hemopoietic stem cells (CFU-S Spleen colony-forming unit cells), presumptive prothymocytes (TdT-positive bone marrow cells), extramedullary hemopoiesis, and peripheral T and B cells. All of these effects appear to be mediated by a humoral factor, or factors, that accumulates in the serum between 1 and 6 h after treatment. Normal syngeneic recipients of 0.25—0.5 ml of this serum show changes in their lymphohemopoietic systems 3 to 8 days later. Serum fractionation on Sephadex G-25 indicates that the transferred activity is not due to residual TBZ or its major metabolites, but rather to an induced factor. We propose that this factor results from the interaction of stimulated macrophages and T cells. Pending further characterization, we have designated this factor LHSF, lymphohemopoiesis stimulating factor.

ISSN:0732-6580    

出版商:OVID    

年代:1982

数据来源: OVID

ISSN:0732-6580    

出版商:OVID    

年代:1982

数据来源: OVID