摘要:

SummaryAn automated method for measuring β‐hydroxybutyrate was adapted to the Ciba‐Corning 550 Express™ random access analyzer. The assay was based on a kinetic reaction utilizing hydroxybutyrate‐dehydrogenase. β‐hydroxybutyrate concentration (mmol/L) was calculated ratiometrically using a 1.0 mmol/L standard. Canine serum, plasma, and urine were used without prior deproteinization and only a 30‐μl sample was required. The method demonstrated good linearity between 0 to 2 mmol/L of β‐hydroxybutyrate. Analytical recovery (accuracy) within these concentrations ranged from 85.8 to 113.3%. Both within‐run and day‐to‐day precision were determined, as was specificity of the assay in the presence of a variety of interfering substances. The automated assay was rapid and economical, with reagent stability maintained for at least 2 weeks at 4° C. This assay can readily be applied toward the assessment of ketoacidosis in dogs, and with further v

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1992.tb00573.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1992.tb00574.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryA Baker 9000 hematology analyzer (electronic impedance) was purchased to replace an Ortho ELT‐8/ds analyzer (laser optics) due to discontinued technical support. An analytical comparison of hemograms from healthy dogs, rats, and mice was made from paired disodium ethylenediamine tetra‐acetate anticoagulated blood samples. Both instruments were calibrated with human blood products, and the ELT‐8/ds hematocrit (HCT) was calibrated to a spun packed cell volume (PCV) for each species. For Beagle dogs (n = 49), Baker 9000 mean platelet (PLT) counts had a negative bias of −89 × 103/μl when compared to ELT‐8/ds values. Mean ± standard error manual PLT counts compared well with Baker 9000 values for dogs (n = 10): 369 ± 28 vs. 367 ± 27 × 103/μl; r = 0.93. For CD‐1 mice (n = 44), Baker 9000 mean white blood cell (WBC) counts had positive biases of 1.1 × 103/μl when compared to ELT‐8/ds and 0.5 × 103/μl when compared to hemacytometer counts. Diluted microsamples using the predilution mode on the Baker 9000 compared well with undiluted samples for mice. For Sprague‐Dawley rats (n = 70), Baker 9000 mean WBC, red blood cell (RBC), and PLT counts had absolute biases of 0.8 × 103/μl, −1.09 × 106/μl, and −357 × 103/μl, respectively, when compared to ELT‐8/ds values. Baker 9000 RBC, WBC, and PLT counts from rats compared well with reference hemacytometer counts. The Baker 9000 HCT determination for rats had an absolute negative bias of 6% when compared to the ELT‐8/ds values or spun PCV. The Baker 9000 required whole blood calibration to PCV for accurate determination of HCT for rats. The biases between analyzers may be due to inherent physical differences between the analytical m

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1992.tb00575.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummarySerum biochemical and hematologic values were obtained from Rhesus monkeys (Macaca mulatta) before and 15 minutes after intramuscular injection with ketamine hydrochloride (5–10 mg/kg). A 345% increase in serum creatine kinase activity 15 minutes after ketamine administration was attributed to muscle damage caused by the injection. Decreases in erythrocyte count, hemoglobin, hematocrit, total leukocyte count, lymphocyte count, and the serum concentrations of glucose, total protein, albumin, and other serum analytes were all attributed to the reversal (by ketamine) of the excitement or “alarm reaction” associated with physical restraint. The decrease in circulating erythrocytes and lymphocytes indicated a redistribution of these cells from the circulating blood to the spleen and extravascular sites, respectively. Decreases in concentrations of total protein, albumin, and several other serum analytes suggested an influx of fluid into the vascular space. The decrease in glucose may also reflect the reversal of an epinephrine‐induced hyperglycemia in the excited awake monkey. These alterations should be considered when designing studies and interpreting data for Rhesus

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1992.tb00576.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummarySerial serum electrophoreses and routine serum protein assays were used to assess changes in serum protein concentrations after severe thermal injury in a dog. Electrophoretic patterns during the month of evaluation were consistent with protein‐losing dermatopathy (thermal burn) and inflammatory dysproteinemia

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1992.tb00577.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryAn enzymatic, kinetic method for determining serum lipase activity was evaluated and compared to a standard manual method for use in dogs. The kinetic method was a commercial kit adapted for use on a tandem access clinical chemistry analyzer and utilized a series of coupled enzymatic reactions based on the hydrolysis of 1,2‐diglyceride by lipase. The manual method was the Cherry‐Crandall technique based on the titration of base against the acid formed by hydrolysis of an olive oil substrate by lipase. The correlation between the two methods was very good (r = 0.94). The reference range for 56 clinically healthy dogs assayed by the kinetic method was 90 to 527 U/L. Diseases associated with a greater than twofold elevation in serum lipase activity as determined by the kinetic method included pancreatitis, gastritis with liver disease, and oliguric renal failure with metabolic acidosis. In some cases, pancreatitis was seen with other clinical problems, such as gastroenteritis, diabetic ketoacidosis, duodenal mass, disseminated intravascular coagulation, and septic peritonitis. Diseases associated with serum lipase activity within the reference range or elevated less than twofold included gastritis, gastric ulcer, cholestasis, phenobarbital‐induced hepatopathy, colitis, copper hepatopathy, abdominal hematoma, apocrine gland adenocarcinoma, and thrombocytopenia with pneu

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1992.tb00578.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1992.tb00579.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY