ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1985.tb00838.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1985.tb00839.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1985.tb00840.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SummaryNormal reference values for serum proteins of foals from birth to 1 year of age have been established. Chemical and electro‐phoretic/refractometric methods for total protein, albumin, total globulin and Albumin/Globulin (A/G ratio) have also been compared.The biuret total protein method and Brom‐cresol Green (BCG) method on the Techni‐con SMA 12/60 autoanalyzer were used and compared with total protein determined via refractometry and albumin determined by Cellulose Acetate (CA) electrophoresis/den‐sitometry. Globulin and A/G ratios were calculated from the chemical method data and compared with that obtained electrophoreti‐cally. Total protein, albumin, total globulins and A/G ratios all were in agreement at all sample times studied.Data on the subfractionation of serum globulins via CA electrophoresis is also presented. Wide variations in β and γ globulin levels were noted among the foal sera early in life. As a result, two distinct populations of foals with respect to both globulin content and A/G ratio were identified. One of these populations (Group A) appeared to have obtained passive immunity more slowly than the other (Group B) animals. Comparison of these data with clinical cases of foals in which failure of passive transfer was a part suggests that the A/G ratio may be useful in assessing adequate colostral antibody levels in the n

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1985.tb00841.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SummaryThe effects of three phlebotomy techniques — periorbital sinus puncture, tail vein incision and cardiac puncture — were determined on seven hematological parameters and seven clinical chemical parameters in female Sprague‐Dawley rats. The mean er‐ythrocyte count, leukocyte count, hemoglobin and hematocrit were reduced with cardiac puncture as compared to the other two techniques. There was a statistically significant increase in the variance of each of these parameters except the leukocyte count. The mean serum lactate dehydrogen‐ase, aspartate animotransferase, alanine aminotransferase, gamma‐glutamyl transfer‐ase and creatinine were greater in samples collected by cardiac puncture than with the periorbital sinus and tail vein techniques. A statistically significant difference in variance was observed between the orbital sinus puncture and the cardiac puncture for each of these parameters. In all cases except lactate dehydrogenase, the values from the orbital sinus and tail vein techniques were comparable for both hematology and clinical chemistry. A large variance was found by all three techniques for both alkaline phos‐phatase and lactate dehydrogenase in the rat. Over 60% of the serum samples were hemolyzed from the cardiac puncture technique while approximately 25% of the serum samples from tail vein incision were hemolyzed. In this laboratory the lack of hemolysis and the lower variance make the periorbital sinus venipuncture technique the method of choice for collection of blood sa

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1985.tb00842.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SummaryRenal percent clearance ratios for various electrolytes were determined on nine clinically normal Holstein heifers. Endogenous creatinine serum and urine levels were used to calculate the ratios. The average percent clearance ratios and standard deviations of Na, K, Cl, P, and Ca were 1.97±0.63, 49.3± 9.2, 3.16±1.12, 15.6±14.3, and 1.38 ±1.41, respectively. The correlation between Na and Cl percent clearance ratios within a sample was 0.92. A very strong direct correlation of urine creatinine and urine specific gravity was demonstr

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1985.tb00843.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SummarySiderotic granules were recognized in blood erythrocytes from a male Boxer dog with suppurative prostatitis, cystitis and pyelonephritis that was being given high dosage chloramphenicol therapy. Siderotic inclusions were recognized in the cytoplasm of 96% of the rubricytes and metarubricytes in a bone marrow aspirate. Siderotic inclusions were numerous and in some cases formed a ring around the nucleus. This peri‐nuclear location suggested that pathologic mitochondrial iron accumulation had occurred, resulting in the formation of “ringed” sideroblasts. The occurrence of pathologic sideroblasts was confirmed by electron microscopy. Blood siderocytes and bone marrow sideroblasts disappeared after cessation of chloramphenicol th

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1985.tb00844.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY