ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1986.tb00859.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1986.tb00860.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SummaryThe precision and stability or the ion exchange chromatography assay for canine glycosylated hemoglobin (HbA1) were examined. The coefficient of variation (CV) of within‐run replicate assays was 1.3 to 2.6%; the CV of between‐run duplicate assays was 3.1%. The mean HbA1content in 44 healthy dogs was 7.1% (SD = 1.1%, range = 5.1–9.7%). Paired aliquots of 12 blood samples were stored at 4° and 25°, and HbA1was measured on the day of collection and at 3, 5, and 7 days aftercollection. In the blood stored at 4°, no significant increase in the HbA1content was seen. No significant increase in HbA1content was found in the blood stored at 25° after 3 days, but dramatic increases were observed after 5 and 7 days of storage. No significant difference was observed in the HbA1content in heart blood collected 18 hours after death from 9 dogs kept at 25°C.The HbA1content was measured in 10 hospitalized diabetic dogs. Five of the dogs had received no insulin and all 5 had elevated HbA, values. The other 5 dogs had received insulin for 1 to 9 months; 2 of the 5 had increased HbA1content. The HbA1content was determined periodically for 9 months in one diabetic dog and it declined from 14.

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1986.tb00861.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SummaryNineteen dogs were identified that had mastocytemia (mast cells in venous blood samples) not associated with mast cell neo‐plasia. The first 10 dogs were identified by examination of blood films from dogs with suspected parvovirus enteritis (8), fibrinous pericarditis and pleuritis secondary to thoracic lacerations (1), and renal insufficiency of unknown cause (1). Because of the apparent association with acute enteritis, blood films from 52 suspected canine parvovirus cases were examined retrospectively and 8 mastocytemic dogs were found. An additional 52 canine blood films were randomly selected from the same retrospective time period and 1 mastocytemic dog was found that had pneumothorax, pelvic fractures, and hemorrhagic septic abdominal effusion secondary to renal hemorrhage and traumatized intestines. All mastocytemic dogs had acute inflammatory leukograms the day that mast cells were first detected: neutropenia with toxic neutrophils (4), neutropenia with a left shift (8), total neutrophil count within reference interval but with a left shift (5), or neu‐trophilia with a left shift (2). All dogs except the renal insufficiency case had circulating toxic neutrophils. Five dogs were mastocytemic on more than 1 day. The pathogenesis of the mastocytemia associated with the acute inflammatory disease was not determi

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1986.tb00862.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SummaryA competitive enzyme immunoassay has been used to detect and quantitate fibronectin in canine plasma. In this test, purified fibronectin, bound to microtiter plates, competes with plasma fibronectin for the conjugated antibody, rabbit‐anticanine, fibronec‐tin‐horseradish peroxidase. The assay could detect fibronectin in purified standards from 58 ng/ml to 580 μg/ml. The range of 1–100 μ g/ml was linear for plasma samples diluted 1:10, allowing samples with fibronectin concentrations from 10–1000 μg/ml to be easily measured by this method. The mean normal fibronectin concentration of 132 dogs, by this method, was determined to be 320

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1986.tb00863.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY