摘要:

Serum sorbitol dehydrogenase (SDH) activities in 10 cows and nine horses were measured using an automated clinical analyzer. The serum samples were divided into aliquots that were stored at room temperature (21°C), refrigerated (0–5°C), or frozen (‐30°C). The stability of the SDH activity was monitored at various intervals. SDH activity in bovine sera remained stable for at least 5 hours at room temperature, 24 hours refrigerated, and 72 hours frozen without any significant (p ≤ 0.05) differences from the initial serum values. In equine sera, SDH activity remained stable for at least 5 hours at room temperature and 48 hours frozen. The activity of the refrigerated equine sera was stable for at least 5 hours but less than 24 hours. An evaluation of fresh bovine serum and heparinized plasma samples indicated that there was no significant difference (p ≤ 0.05) between the two sampling methods and that either may be employed for automated measurement of SDH activity following the established protocol. Sample type comparison indicated that there was a small but statistically significant (p ≤ 0.05) difference between the results obtained comparing fresh serum and heparinized plasma samples for the horse. A reference range for Holstein cows was established using sera from 71 clinically healthy cattle (mean ± 2 SD =

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1993.tb00869.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Nine combinations of dosages and concentrations of D‐xylose were given orally to eight clinically normal, immature dogs. The concentrations and dosages of D‐xylose consisted of 5%, 10%, and 20% at 250 mg/kg, 500 mg/kg, and 750 mg/kg. Serum samples were collected at 0, 30, 60, 90, 120, and 180 minutes. Serum xylose was quantitated using the phloroglucinol microassay technique. A peak in serum xylose concentration was seen for each treatment combination at 60 or 90 minutes after dosing. The dosage effect was important in influencing serum xylose values (P<0.0001). As the test solution dosages increased from 250 mg/kg to 500 mg/kg and 750 mg/kg, serum xylose values (when dosage was analyzed over the length of the entire test) rose linearly (R2= 0.98). The treatment combinations of 5% and 20% xylose solutions dosed at 750 mg/kg produced the highest serum xylose values at the 60‐and 90‐minute peak intervals. The independent effect of concentration was significant (p0.05) when the 5%, 10%, and 20% solutions were compared at a specific

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1993.tb00870.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

An assay was developed and proven accurate and precise for the quantification of canine serum alkaline phosphatase of bone origin (BAP). The assay uses wheat germ lectin (WGL) which selectively precipitates BAP but not liver alkaline phosphatase (LAP) in serum preincubated for 1 hour at 37°C before conducting the assay. Although a large percentage of corticosteroid‐induced alkaline phosphatase (CAP) is also precipitated by WGL, the activity of this isoenzyme can be determined by utilizing the automated levamisole inhibition assay and BAP determined by subtraction except in those cases in which CAP is very markedly increased. Use of these two assay techniques in combination allows the quantification of LAP, BAP, and CAP activity in canine serum.In sera from adult dogs of various ages, BAP activity represents a mean of 21.27 ± 11.4 U/L; however, there was a statistical decrease in BAP activity with age. This allowed the determination of 95% confidence interval for a reference range dependent on age of the dog. Bone AP activity in puppies drops dramatically within the first 3 months, reaching a magnitude of activity consistent with that of the adult dog by approximately 15 months. BAP was increased over adult reference range in five of five dogs tested with osteosarcoma. This assay will now allow conducting a clinical study of the diagnostic significance of bone AP activity in neoplastic and metabolic diseases of b

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1993.tb00871.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Cadmium was administered via the drinking water to rats at doses of 20 and 200 ppm for 5 weeks in order to elucidate cadmium's primary effect on the erythrocytic parameters. From the results, it appeared that the microcytic hypochromic anemia resulted from a direct effect on erythrocytic hemoglobin due to cadmium administration. It was proposed that the adverse effects on hemoglobin absorption and metabolism play a role in the development of the anemia observed.

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1993.tb00872.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1993.tb00873.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY