摘要:

SummaryA total of 25 pregnant goats without neutralizing antibodies against BVD virus were inoculated with two different pestivirus isolates at eight different stages of gestation. In both infection groups, various malformations were observed in fetuses and neonates. In three twins with neutralizing antibodies against BVD virus leucoencephalomalacia occurred, characterized by gelatinous transformation in the cerebral hemispheres. These lesions were comparable to alterations described in alternative pathology of Border disease in sheep. Although the immunohistological findings are characteristic for immunological tolerance and viral persistence, viable offspring persistently infected with pestivirus was not observed.

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1992.tb01131.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryA total of 64Pasteurella multocidastrains (46 out of 11 different feral bird species, partly injured by cat bites, and 18 strains originating from clinically healthy cats) were biochemically differentiated. As a result, 67.4% of the strains from feral birds and 61.1 % from the cats were classified as the subspeciesmultocida, whilst 21.7% and 27.8% were identified as the subspeciesseptica. The percentage of frequency for both the subspecies was of a comparable order of magnitude from the birds injured by cat bites and from cats (58.6% and 61.1 %, 24.1 % and 27.8% resp.), whereas the frequency from other feral birds differed considerably (82.4% and 17.6%). Maltose‐positive strains were only demonstrable in birds with wounds inflicted by cats. To date, maltose‐positive strains have only been obtained from one cat and one human being with an injury caused by a cat. The results of this investigation confirm the possibility of the direct transmission ofPasteurella multocidavia cat bites. 19 strains from feral birds and 15 strains from cats were tested for their capability to produce toxins. The results of these tests were negative. The present paper also describes the pathologic‐anatomical and histopathological lesions caused by the infection in feral

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1992.tb01132.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe influence of polychlorinated biphenyls (PCBs) on phocine distemper virus (PDV) infections in harbour seals (Phoca vitulina) was studied. Six out of ten seals had been conditioned with a defined mixture of PCB‐congeneres for several weeks. Following exposure to the cell culture‐propagated PDV isolate 2558/Han88 the complete clinical picture of “1988 seal plague” was provoked in all ten seals inoculated. Four out of six PCB‐conditioned seals and two out of four seals not loaded with PCBs succumbed to the infection within three weeks post inoculation. With regard to the clinical course, duration of cell‐associated viremia, PDV‐antigen distribution in tissues of fatally infected seals and the humoral immune response to PDV no differences between PCB‐loaded and unloaded seals were recognized. Evidence was obtained that the pathogenesis of experimental PDV‐infection in harbour seals shares some features with those of canine distemper in terrestrial carnivores. In contrast, however, to experimental distemper infection of gnotobiotic dogs prompt development of high titres of PDV‐specific IgG did not correlate with reco

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1992.tb01133.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryWe established 9 cell lines from 63 tumor cases of enzootic bovine leukosis and studied their properties. Cells of all lines formed small clumps and floated in culture medium, indicating growth. Four of the 9 cell lines were surface immunoglobulin (SIg)‐positive, but the remaining 5 line cells were negative for SIg or, if SIg was detected, the percentage of SIg‐positive cells was very low. Tests for the properties of the cells with monoclonal antibodies to lymphocytes revealed that the established line cells are B‐lymphocytes. Morphological observation also revealed that they had the morphology of B‐lymphoblastic cell. The results of E and EAC rosette assay were negative, but 6 of 8 cell lines were positive for EA rosetting. All the 9 cell lines reacted with MoAb C‐143, which recognizes the tumor‐associated antigen (TAA) of the EBL tumor cell. All 9 cell lines produced bovine leukosis virus (BLV). These results suggest that the 9 cell lines are tumor cells derived from B‐lymph

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1992.tb01134.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe lytic activity, protein profile and morphology of five newly isolated phages from canineStaphylococcus aureusstrains and one from a humanS. aureusstrain were compared with those of selected phages in the international phage sets (IPS). Five canine phages lysed 57 (76.0 %) of 75 canine isolates ofStaphylococcus aureusfrom Nigeria at routine test dilution (RTD) while 34 (IPS) phages typed only 31 (41.3 %) strains at RTD or/and 100‐RTD. The new human phage lysed 11 (14.7%) of 75 strains isolated from human diarrhoea. The new phages were readily propagated, specific in activity and stable during storage at 4°C.Prominent proteins detected by SDS‐PAGE indicated similarities between some of the phages but one canine phage was distinctly different, as was its morphology which was an isometric head with a short tail compared to oval heads and long tails which characterized others. IPS phages in the same serologic group had similar protein profiles but no correlation was observed with lytic groups. The use of protein profile and electron micrographs allowed classification of the phages into serogroups.It is concluded that the newly isolated canine phages could be very useful in typing Nigerian canine strains ofS. au

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1992.tb01135.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryA sensitive protein immunoblotting (Western blot) procedure has been developed for detecting anti‐BLV antibodies in cattle sera. The antibodies against most of the major viral proteins could be detected. This procedure does not give any non‐specific background staining and there is absence of any erroneous results due to utilisation of purified viral preparations. The procedure has been applied for detection of antibodies to BLV in a set of 74 sera samples and it has been compared with other commonly used serological tests like ELISA and agar gel immunodiffusion t

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1992.tb01136.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummarySerum samples from 224 Norwegian cats were analyzed for the presence of feline leukemia virus (FeLV) p27 common core antigen, and for antibodies to feline immunodeficiency virus (FIV). Ninety specimens originated from the serum bank at the central referral clinic at the Norwegian College of Veterinary Medicine, which had been collected during the years 1983–1989; 67 sera were submitted from veterinarian practitioners; while 67 sera originated from cats presented for euthanasia. The cats were classified into one “healthy” and one “sick” group.Only 2.2 % of sick cats and 1.2 % of healthy cats showed FeLV antigenemia, a finding which is lower than which has been reported from many other countries.The prevalence of FIV antibodies was 10.1 % in sick cats and 5.9 % in healthy cats. Antibodies to FIV was most prevalent in male cats (14.7%) than in female cats (2.1 %), and more prevalent among domestic cats (12.0 %) compared to pedigree cats (2.4 %). Antibodies to FIV in the cats demonstrated increasing prevalence with increasing age. It may be concluded that FeLV causes minor problems in Norwegian cats, while FIV is present in a similar prevalence to what is reported form other

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1992.tb01137.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryIn the electron microscope an additional layer (glycocalix) of the cell wall and fimbriae onHaemophilus parasuiswere shown in thin sections of the infected CAM which have their origin on the CM of theHaemophilus parasuis‐cells. No fimbriation was seen after conventional cultivatio

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1992.tb01138.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe ratio of the different types ofS. aureussurviving cells immediately after sub‐lethal heat treatment (52 °C for 15 min) was studied. A high ratio of cells which are unable to form colonies even in a common medium such as TSA was observed. This fact has not been reported previously for common media through recovery after heat shock has been widely studied. After the treatment, staphylococcal protein A, but no enterotoxin, was seen to be released into the extracellular environme

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1992.tb01139.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryA double polymerase chain reaction (PCR) assay has been devised for the direct detection of bovine leukemia virus (BLV). The assay was directly performed on blood leukocytes, avoiding the DNA‐purification procedures. The PCR products were identified by gel‐electrophoresis and the specificity of the test was confirmed by hybridization with a biotinylated oligonucleotide probe. When testing the sensitivity of PCR, less than eight genome copies of the provirus were detected in the background of two million negative lymphocytes.In a BLV infected herd 22 animals of various age groups were examined by the indirect (serological) diagnostic tests of agar‐gel immunodiffusion and indirect ELISA as well as by the direct detection method of PCR. The tests were repeated at monthly intervals on five occasions. When examining the specimens from cows and heifers, a close agreement was found between the results of the various methods. The newborn calves, which were the offspring of BLV infected mothers, were consequently negative in PCR throughout the experimental period. However, in the indirect tests the calves were positive during the first samplings and became negative only around four months of age. Since the indirect tests can not discriminate infection from colostral immunity, PCR proved to be a useful complementary assay for the safe diagnosis of BLV infection in young c

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1992.tb01140.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY