摘要:

SummaryThe modified live canine parvovirus (CPV) vaccine was used to vaccinate intranasally twenty‐five pups with maternal antibody. The vaccine was able to overcome the interference of maternal immunity in rates of 100 %, 72.7 % and 17.6 % in pups with haemagglutination inhibition antibody titre of 40, 80 and 160 respectivel

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1994.tb00199.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryThis study deals with the determination of 2,3‐DPG concentrations in canine blood. The results obtained demonstrate the intraerythrocytic location of this organic phosphate as well as its equimolar relationship with the haemoglobin tetramers in blood obtained from clinically healthy dogs. The average value obtained for this group of healthy dogs (n = 93) was 5.81 ± 0.07 mmol of 2,3‐DPG/L of erythrocytes. In dogs with cardio‐pulmonary insufficiency (n = 12) and uraemic syndrome (n = 10), a greater concentration of 2,3‐DPG was observed, together with a loss of the equimolar relationship between this phosphate and the Hb tetramers. Furthermore, in the group of dogs with uraemic syndrome we observed a highly significant correlation between the blood values of urea and the concentration of 2,3‐DPG expressed in relation to the haemoglobin

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1994.tb00200.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummarySome important biological and physico‐chemical characteristics of porcine circovirus are reported. These include a study on the host distribution in nature, levels of colostrum‐derived antibodies in piglets from sero‐positive sows and the susceptibility of a range of cell cultures to infection with this virus. The results of haemagglutination studies, resistance to pH 3, chloroform and heat are also reported as are comparative buoyant densities and sedimentation coefficients of porcine circovirus and chicken anaemia

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1994.tb00201.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryThis paper presents the results of a study of eight cases of natural bovine respiratory syncytial virus (BRSV) infection in a herd of 50 Murciana kids from a intensive goat‐farm in Spain.Clinical and pathological analysis confirmed the existence of natural BRSV infection in eight kids. In two of the eight animals there was also evidence of concurrent infection withP. haemolytica

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1994.tb00202.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryEight monoclonal antibodies (MAbs) were tested for the use in immunogold staining of Sindbis virus (SIN) antigen in cultured Baby Hamster kidney (BHK) cells. The antibodies were directed against the capsid (C) protein (5) or against the glycoprotein E1 (3), respectively. Four out of five anti‐C MAbs and two out of three anti‐E1 Mabs reacted on frozen sections, whereas two of the anti‐C antibodies and none of the anti‐E1 antibodies reacted on Lowicryl K4M‐emedded cells. The MAbs reacting, in particular the anti‐C Mabs which react on Lowicryl K4M‐sections, will allow further studies of the pathogenesis and replication of alphaviruses on the cellular level. The results also confirm the conformation‐dependence of the glycop

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1994.tb00203.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryEleven entire and twelve castrated male pigs were implanted with trenbolone acetate and the effects of the treatment on genital tract macro‐ and microscopical modifications were evaluated and compared to non‐implanted animals. In entire male pigs a reduction of testicular weight and size was observed as a consequence of the treatment (<0.001), but no important modifications were found in bulbourethral gland and seminal vesicles macroscopically. Castrated non‐implanted males had bulbourethral gland atrophy (P<0.01), while castrated implanted ones showed similar development to entire males. As for vesicular glands there were no significant differences among the four groups under study. Histological study of prostate has demonstrated structural modifications. As a consequence of the treatment glandular lumen was increased (P<0.05), and most parenchima was occupied by polimorphous

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1994.tb00204.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryTwo different genotypic methods, colony hybridization and polymerase chain reaction (PCR), were applied to detect enterotoxin, verotoxin and fimbrial genes in 708Escherichia coli (E. coli)strains from piglets with diarrhoea. The results were compared with those obtained by phenotypic methods.DNA fragments specific for each of the enterotoxins LT, STaand STb, the verotoxins VT1, VT2and VT2v, and for each of the fimbrial genes K88 (F4), K99 (F5), 987P (F6), F41 and F107, respectively, were used as probes in a colony hybridization assay of theE. colistrains. A PCR assay was used as genotypic test for the verotoxin gene. An Enzyme‐linked immunosorbent assay (ELISA) using monoclonal antibodies was performed to test for the presence of K88 and K99 fimbriae, and a Vero cell test was performed to test for verotoxin production. Toxin detection kits were applied to detect theE. coliheat‐labile enterotoxin (LT) and the heat‐stable (STa) enterotoxin.The correlation between the results obtained by genotypic and phenotypic methods was 97.7–100 %. The prevalence of the different fimbrial and toxin genes inE. colistrains from piglets with neonatal and postweaning diarrhoea were recorded. The verotoxin and the fimbrial F107 genes were found to be more frequent in postweaningE. colistrains than in neonatalE. coli

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1994.tb00205.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryNorfloxacin nicotinate (NFN) is a new water‐soluble fluoroquinolone antibacterial agent. Thein vitroactivity of NFN for microorganisms isolated from swine and the pharmacokinetic properties of NFN following single intravenous (i. v.), intramuscular (i. m.) and subcutaneous (s. c.) administration were investigated.The minimal inhibitory concentrations (MIC's) of NFN for a wide range of reference (ATCC ‐ American Type Culture Collection) microbial swine isolates, comprising 21 bacterial and 5 mycoplasmal species, ranged between 0.03 μg/ml (forSalmonella cholerasuisandActinobacillus pleuropneumonia) and 12.5 μg/ml (forStreptococcus porcinus). The MIC of NFN forMycoplasma hyopneumoniae, the causative agent of enzootic pneumonia of pigs, was<1.0 μg/ml althoughM. hyosynoviaewas less sensitive, with a MIC value of 3.12 μg/ml. The MIC values of the drug for swine field isolates, comprising 8 bacterial species, were in good agreement with the values determined for the corresponding ATCC strains. Pharmacokinetic values for NFN were calculated following i. v. administration at 7.0 mg/kg and i. m. and s. c. administration at 14.0 mg/kg in a 3‐way cross over study involving 6 pigs. Plasma concentrations of unchanged drug were determined by HPLC during 24 h post injection. Plasma NFN concentrations measured after i. v. dosing best fitted a 2‐compartment open system pharmacokinetic model. The harmonic mean distribution half‐life (t1/2α) and elimination half‐life (t1/2β) were 4.2 minutes and 2.1 h, respectively. The mean residence time (MRT) was 2.9 ± 0.6 h and the steady state volume of distribution (Vss) was 3.2 ± 0.1 ***l/kg. The mean t1/2βvalues after either i. m. or s. c. administration were 4.45 h with very rapid absorption rates (<15 min to peak plasma drug concentration). Bioavailability was 51–64 %. Less than 20 % and 25 % of the dose were excreted in the urine as parent drug during the first 24 h after i. v. and s. c. dosing, respectively whereas the amount of unchanged drug recovered in the feces was very small (1.3 to 1.6 % of the dose).The pharmacokinetic and MIC data generated in the course of the present study suggest that a dose schedule of 14.0 mg/kg injected i. m. or s. c. every 24 h is capable of achieving and maintaining tissue drug concentrations of potential therapeutic value for the treatment of the most common bacterial and mycoplasm

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1994.tb00206.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryA slow‐spreading mild form of camel‐pox involving range camels in the Eastern region of Saudi Arabia was described. The morbidity rate was 10 % while the case fatality rate was zero per cent. Camel pox virus was isolated and identified, and the histopathological picture of the lesions was also described. The epidemiological peculiarities of the disease were discus

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1994.tb00207.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Book reviewed in this article:Zipfel, W., Lebensmittelrecht. Loseblatt‐Kommentar der gesamten lebensmittel‐ und weinrechtlichen Vorschriften sowie des Arzneimittelrechts.Zipfel, W., Lebensmittelrecht. Loseblatt‐Kommentar der gesamten lebensmittel‐ und weinrechtlichen Vorschriften sowie des Arzneimitte

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1994.tb00208.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY