摘要:

Rabbit aortas were examined by light, scanning, and transmission electron microscopy to observe the morphological steps in focal endothelial cell desquamation. The aortas were not subjected to any instrumentation prior to fixation by either immersion alone or combined perfusion and immersion. In areas where boundary layer separation of flow with eddy formation is known to occur, many elongated protruding cells were observed, some obviously partly loosened from the basement membrane. Some of the latter cells appeared to be attached by two ends and to be twisted longitudinally. Single cells, or even sheets of cells, were completely detached. The breaks seemed to have taken place close to, and parallel with, intercellular junctions, but not within these structures. Platelets had reacted both with injured cells and the denuded intimal surface. Thus, the sequence appears to be protrusion of cell body, partial loosening of cells from the basement membrane, breaks near the intercellular junctions, twisting of cells, and complete detachment.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1978.tb02005.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

A 52‐year‐old woman with vaginal bleedings had hysterectomy because curettings prompted suspicion of malignancy. A uterine tumour with deep infiltration of trophoblastic cells in the myometrium was revealed. Postoperative quantitative analysis for urinary human choriongon‐adotropin gave 1500 IU/1. The patient got no other treatment and is well 3 years after operation. The histopathological picture and the clinical course were consistent with the recently introduced diagnosis of benign “trophoblastic pseudotumour”, which should perhaps replace the old terms “syncytioma” and “chori

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1978.tb02006.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

The occurrence of extracellular polyamines in Ehrlich ascites tumour‐bearing mice was studied in relation to tumour growth. The concentration of putrescine and spermidine in cell‐free ascites fluid and serum was found to increase significantly with increasing tumour mass. Data is presented which suggest that the observed accumulation of extracellular polyamines is a result of a continuous release from dead or dying tumour cells. This observation is consistent with the notion that extracellular polyamines accumulate during tumour growth and following radio‐ and chemotherapy as a result of tumour cell death, and emphasizes the potential clinical usefulness of the polyamine analysis for evaluating tumor cell

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1978.tb02007.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Only four of the nine published cases of solitary, extraskeletal, intracranial plasmacytoma are considered authentic. In this report we describe an accidentally discovered dural plasmacytoma and draw the attention to this rare lesion which might have a different prognosis from the rather benign course of meningioma with which it is confused.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1978.tb02008.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

All the malignant soft tissue tumours in the extremities and limb girdles reported to the Finnish Cancer Registry between 1960–1969 were reviewed. From a total of 246 sarcomas, 38 were diagnosed as malignant fibrous histiocytoma. There was an equal number of male and female patients with the median age of 67 years. The thigh was the most frequent site, and the majority of the tumours originated in the deep soft tissues. The predominant treatment was excision followed by radiation therapy. In 17 patients there were one or more recurrences and in 21 patients there was a metastatic spread ascertained by biopsy, autopsy or clinical or radiographic evidence. There were 11 survivors with a minimum of 5 years' follow‐up;seven patients died of an intercurrent disease and the remaining 20 patients were considered victims of their tumour. The findings that seemed to favour a poor prognosis were higher age and female sex of the patient as well as deep location, large size, necrotic areas, and high mitotic activity of the tum

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1978.tb02009.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

A preliminary study, based on officially recorded cases of cervical carcinoma, dysplasia and carcinoma in situ in Greenlandic women over the period 1955–1975 revealed a total of 117 cases of carcinoma and 119 cases of dysplasia and carcinoma in situ. The incidence of invasive carcinoma, age‐adjusted to “European standard population” (Doll1976), exhibited a rise from 20.9 per 100,000 in 1955–1959 to 84.8 in 1970–1975. The latter incidence rate is one of the highest in the world, and about 2.5 times as high as the most recently recorded incidence in Denmark. Age‐specific incidence rates for cervical carcinoma in Greenland for 1970–1975 showed significantly higher values in the age groups 15–49 and 60–64 years as compared with the most recent Danish rates. The crude incidence of dysplasia and carcinoma in situ per 100,000 women over 15 years of age rose from 10.5 for 1955–1959 to 137.5 for 1970–1975. The corresponding incidence for severe dysplasia and carcinoma in situ was 93.7 for 1970–1975. There has most likely been a more complete registration of these initial stages during recent years, although organized detection wo

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1978.tb02010.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Human glioma cells, growing as spherical colonies in agarose gel, or as monolayers on glass or plastic, were studied with time‐lapse cinematography and electron microscopy. The cells in the agarose cultured colonies often had ruffling‐like membrane structures which were similar in for, although smaller in size, than those observed on monolayer cultured cells. The ruffling‐like structures were more frequent at the periphery than in the central regions of the colonies which was in parallel to the proliferative pattern. In time‐lapse cinematography it was seen that pinocytotic vacuoles were formed from ruffling membranes in the monolayer cultures. In the transmission electron microscope, such vacuoles were also found near the ruffling‐like structures in the agarose cultured cells. In dense monolayer cultures, ruffling and associated pinocytosis were to a large extent transferred from the margin to the upper surface of the cells. This capacity may be an important property for the ability of the malignant cells to attain extreme high cell densities in monolayer cultures and to grow as colonies in agarose cultures. As has been previously shown, the normal counterpart of the gliomas, the glia cells, cannot grow to high densities; they do not ruffle on their upper cell surface and are unable to grow in suspension or agaros

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1978.tb02011.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Mosekilde, L.&Melsen, F. Morphometric and dynamic studies of bone changes in hypothyroidism. Acta path. microbiol. scand. Sect. A,86:56–62, 1978.Bone biopsies were performed after tetracycline double‐labelling by transfixing the right iliac crest in fourteen hypothyroid patients. The bone changes in cortical and trabecular bone were determined by simple measurement and point counting on decalcified and undecalcified stained sections and compared to normal controls and to sex and age matched hyperthyroid patients. The amount of trabecular bone and the cortical porosity were unchanged in the hypothyroid patients compared to normal controls, whereas the mean cortical thickness was increased. The amount of osteoid and the length of the osteoid seams were normal, whereas the mean width of the osteoid seams was decreased. The linear calcification rate in cancellous bone was decreased, as were the active calcification surfaces (tetracycline‐labelled) and the percentage of osteoid covered surfaces active in mineralization. The osteoclastic resorption surfaces were unchanged in trabecular bone, whereas the osteoclastic activity in cortical bone was decreased. The osteocytic osteolysis was normal. The bone changes in hypothyroidism were opposite to the changes in hyperthyroidism characterized by a very low bone turn‐over with a reduced osteoid apposition and bone mineralization rate, an inactive osteoclastic resorption in trabecular bone and a decreased osteoclastic resorption in cortic

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1978.tb02012.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

An evaluation of the following histomorphometric parameters: absolute trabecular bone volume (ATBV), osteoid surfaces (OS), osteoid volume (OV), resorption surfaces (RS), and mean size of periosteocytic lacunae (POL) was carried out. Double determination on sections, obtained from an area 2 cm below the summit and 2 cm behind the anterior, superior, spine of the iliac crest from 20 normal individuals aged 20–40 years, showed that the precision of method by far outweighed the inter‐individual variation. Parameters obtained from 9 different localizations within the iliac bone revealed that the volume of bone was the only parameter which was influenced if the bone specimen was removed more than 2 cm below or behind the standard localization. Randomly selected sections alternately stained with Masson trichrome and toluidin blue were used for the study of the influence of the staining method on the osteoid parameters. It was shown that Masson trichrome staining would generally result in a slightly higher value of osteoid surfaces. There was, however, a high correlation between the parameters obtained with the two methods. (OS: r = 0.96; OV: r = 0.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1978.tb02013.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Bone specimens from a standardized area of the iliac crest were obtained at autopsy in 105 individuals after sudden unexpected death and at biopsy in 30 living volunteers. Seven micron thick sections of the plastic embedded undecalcified material were produced and stained. The amount of cancellous and cortical bone and the parameters attached to description of bone remodelling were determined by point counting and simple measurements, in order to establish normal mean values and range in a Danish population. A decrease in the amount of bone, trabecular as well as cortical, was found with increasing age in both males and females. The extent, volume and width of osteoid seams and the osteoclastic resorption were found to be age‐independent. The osteoclastic resorption in cortical bone in males decreased, however, with increasing age. The reported values are of the greatest importance for the use of histomorphometric analysis of bone biopsies as a diagnostic tool in metabolic bone diseases. It is stressed, however, that supplementary information of the dynamic aspects of the bone remodelling would be necessary for the understanding of the pathogenesis of bone changes. Such information might be obtained by using tetracycline double labellin

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1978.tb02014.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY