摘要:

A uterine adenocarcinoma was studied by both chromosome and nuclear DNA analysis. The primary tumour was characterized by a pseudodiploid stemline, and one of the metastases in the mesenterium revealed a hypotetraploid mode and also a smaller near‐triploid pea

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03820.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

The functional structure of the normal rat liver was investigated in rapidly frozen, freeze‐dried tissue. The mean vascular volume of the liver was 27 per cent contrary to conventionally fixed tissue, in which this volume was 7 per cent. In the freeze‐dried tissue the sinusoids were systematic, orientated radially out to the periphery of the lobule. The bile capillaries were readily apparent contrasting to those of the delayed‐frozen or fixed tissue. A significant intracellular oedema of the liver cells was found to appear a few minutes after the cutting off of the blood s

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03821.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

In rats with carbon tetrachloride induced cirrhosis the liver was investigated in rapidly frozen, freeze‐dried specimens. In the rats to which the administration of carbon tetrachloride was stopped 10 days prior to sacrifice the relative sinusoidal volume was found to be higher in the cryo‐preparations (mean 24 per cent) compared to formalin fixed tissue (mean 10 per cent) due to vascular collapse and a rapid development of intracellular oedema in the liver cells as apost mortemalteration in the formalin fixed tissue. This is parallel to previous findings in normal rat livers. In the group of cirrhotic rats treated with carbon tetrachloride until 24 hours before sacrifice necrosis of the liver cells was frequently found. In this group the relative vascular volume was much lower (mean 14 per cent) than in the rats without necrosis (mean 24 per cent) in the cryo‐preparations. These changes in sinusoidal volume, not visualized in conventional histological preparations, are explained by the presence of intracellular oedema of the liver cells caused by the carbon tetrachloride intoxication. In this group of rats the bile canaliculi were poorly visualized compared to the rats without recent intoxication. It is concluded that the use of an appropriate cryo‐technique is essential for the evaluation of the vital alterations in the cirrhotic rat liver, which is only poorly depicted by conventional histological te

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03822.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

The intrahepatic biliary system in congenital hepatic fibrosis has been evaluated by means of stereology and reconstructions in a material from 8 patients. The ductal system was found to consist of a characteristic mixture of different ductal shapes including very flattened, plate‐like ducts. The arrangement of these ducts is in irregular networks, usually organized in separate planes and often completely or incompletely surrounding vessels. No change in the composition of the ductal mixture with age was found. The biliary system is fundamentally identical in congenital hepatic fibrosis and in infantile polycystic disease. It is not possible to determine from the present study whether or not there may be a minor systematic difference between the ductal lesion of the two diseases. The pathogenesis is discussed on the basis of the anatomical findings and the flattened ductal shape is considered to be due to a failure in the embryological development of the duct

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03823.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

A marked degree of Periarteritis Nodosa was found in the kidneys, with fewer lesions in heart and pancreas, in unilaterally nephrectomized haired NMRI mice treated with desoxycorticosterone acetate (DOCA) and 1 per cent saline as drinking water. Similarly treated Nude littermates with congenital aplasia of the thymus failed to develop vascular lesions.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03824.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Anin vitrotest model was used to study the heterogeneity of tumours with respect to the sensitivity to cytostatic drugs. Methylcholanthrene‐induced sarcomas in mice were used. The effect of the drugs was determined as the reduction of the incorporation of H3‐thymidine into drug‐treated cells compared with control cells. To test the heterogeneity of the tumours, these were cut into four equal parts and each part was tested separately. In some of the tumours, the quarters differed significantly in their sensitivity to the same cytostatic treatment; in others, no such differences could be found. Both primary and serially transplanted tumours were studied. The present method revealed no differences in heterogeneity of the tumours in these two groups. The most likely explanation of these results is that the tumours are built up by different clones with different sensitivity to cytostatic drugs and that different parts of the tumours are different with respect to the distribution of c

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03825.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

One primary and one serially transplanted methylcholanthrene‐induced mouse sarcoma was studied for the existence of cell clones that differ in their sensitivity to cytostatic drugs. Two transplantation methods were compared. One method was to graft a small sarcoma piece subcutaneously, a second method was to inject a small volume of a cell suspension prepared from the sarcoma. After a growth period, the transplanted tumours were tested for cytostatic sensitivityin vitro.Reduction in H3‐TdR incorporation measured the effect of the cytostatic drug added. The tumours obtained in the recipient mice differed in sensitivityin vitro.Tumours obtained by grafting material from the serially transplanted sarcoma did not differ in sensitivity more than those obtained by injection transplantation. Tumours obtained by grafting pieces of the primary sarcoma showed a significantly more marked degree of diversity than the tumours obtained by injection transplantation of that sarc

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03826.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

The study concerns comparisons betweennudemice of three genetic backgrounds (BALB/c, C3H&C57/BL) after the third cycle of a gene transfer, and normal members of the respective three inbred strains. The following parameters were considered: body and organ weight, haematological values, differential counts of bone marrow and peripheral blood cells, serum proteins (total and fractionated), and detailed histological appearances of tissues and organs. No thymus tissue was found in anynudemouse. Lymphoid tissue in the spleen, Peyer's patches and lymph nodes was of characteristic histological appearance with lymphocytic depletion. Additionally, marked difference in phagocytic activity in Peyer's patches was observed. Such activity was meagre or absent innudemice. The possible relationship of this finding with low IgA levels is discussed. Peripheral blood lymphocyte counts innudes, irrespective of genetic background, were uniform and consistently very low. It is proposed that these lymphocytes represent a stable B‐cell population. Lymphocyte counts in the bone marrow were somewhat low in allnudes.No quantitative differences in serum protein values could be demonstrated by the methods used. Histological study demonstrated inflammatory changes in one or more organs in 11 of the 24 nude mice, despite the fact that all animals were apparently healthy. No changes were found in the normal mice. The mice were reared under spf (specified pathogen free) conditions. The insufficiency of current spf‐definitions in relation tonudemice is stressed. The infections were always ascending, and frequently involved the genital tract. The significance of possibly impaired IgA secretion is discussed. The genital infections are considered in relation to the infertility ofnudefema

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03827.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

The prevalence of postcapillary venules with high endothelium cells (HE venules) in lymph nodes was studied in biopsy specimens from 18 cases of malignant lymphoma. HE venules were found to be absent in non‐leukaemic lymphocytic and lymphoblastic lymphomas, usually absent but in some cases even abundant in chronic lymphatic leukaemia and finally present and often conspicuous in the specific tissue of Hodkin's disease (lymphocyte predominance, mixed cellularity and nodular sclerosis). In a discussion of the observation it is noted that it can still not be taken for granted that HE venules serve the immigration of recirculating lymphocytes to the lymphatic parenchyma and that the biology of these curious vessels remains to be settle

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03828.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Mice, homozygous for thenugene (nudemice), have no capacity for rejection of allografts as evidenced by this study of nine different graft‐host combinations. The mice concerned stem from an intermediate stage in anugene transfer to three inbred strains (BALB/c, C3H, C57/BL). Mice of each of the three genetic backgrounds accepted skin grafts from three inbred donor strains, differing at the H‐2 locus. A preliminary comparison with a concurrent study of heterografts (rat skin) indicates that allografts are not so readily incorporated, and are more prone to endogenous damage during the take process. This paradoxical observation is discussed at greater length in the subsequent article on heterotransplants innudemice. After allografting,nudemice display lymphocytosis, and there is lymphoid cell proliferation in the primary follicles of lymph nodes, and increased numbers of mononuclear cells are seen in the paracortex of lymph nodes and in the red pulp of the spleen. No cytotoxic alloantibodies could be demonstrated in serum from the graft bearingnudemice. Thymus grafting confers capacity for allograft reject

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03829.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY