摘要:

Secretory ameloblasts in the continuously growing incisors of the rat were used to study the sequential changes in a mature cell type degenerating after administration of vinblastine at a dosage of 2 mg per kg body weight. In less than half an hour nearly all microtubules vanished. This was succeeded by progressive displacement of the nuclei and disorganization of the cytoplasmic structure. After 1 to 3 hours attached and free polyribosomes were converted into monoribosomes. All these cytoplasmic changes were seen in viable as well as in necrotic cell. Between 5 and 6 1/2 hours after application of the drug degeneration of nuclei began; these changes from the outset indicated that a particular ameloblast had been drawn into a sequence of events which would ultimately lead to its death. The progressive alterations of the nucleus and cytoplasm of the degenerating cells and the concurrent fragmentation and elimination of the fragments are described.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00016.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Nuglear segmentation was studied in fine needle aspirate smears from toxic and atoxic goitres. The smear technique implies a flattening of the nuclei on a slide, which facilitates detection of nuclear segmentation. Such segmentation was found in 2.2% of the nuclei in toxic goitres and in 0.9% in atoxic goitres. The difference was highly significant p<0.001. The segmented cells were usually of uniform appearance with one large and one small segment. Only very rarely were more than two segments seen.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00017.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Cellular proliferation was studied by incorporation of3H‐thymidine in the hearts of young, adult and old swimming‐exercised rats. The proliferative activity was measured by scintillation counting. The swimming exercise induced a significant proliferation of capillary wall cells in the younger age groups, suggesting a neoformation of myocardial capillaries in these rats, whereas no cell proliferation was recorded in the old r

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00018.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

The fine structure of three lines of human normal glial cells and eight established lines of malignant glioma cells are described. The glial cell lines were ultrastructurally very similar whereas the glioma cell lines differed greatly from one another. In sparse proliferating cultures there were no consistent findings which distinguished the glioma cell lines as a group from the normal glial cells. Only in post‐confluent cultures could the consistently irregular cell surfaces and ruffling, both at the cell periphery and centrally on the upper cell surface, with associated pinocytosis, distinguish the glioma from the post‐confluent glial cultures, which did not possess these properties. The common attributes of post‐confluent glioma cells reflect the cells' continued proliferation. The glioma lines did display individual ultrastructural characteristics which appear to be stable, the glioma lines having retained these during a number of years of continual pa

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00019.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Three lines of normal human glia cells and eight established lines of malignant glioma cells have been studied in the electron microscope (E.M.), using preparations of critical‐point dried whole cells, sparsely grown on formvar‐coated. E. M., gold grids. The malignant cell lines showed a very varied morphology, almost every line having its peculiarities as compared to the essentially identical normal glia lines. The major differences noted concerned the form of the leading lamellae, number of microspikes and the distribution of organelles such as secondary lysosomes and mitochondria. No single consistent finding made it possible to differentiate the glioma cells as a group from the glia cells in sparse cultures. The findings of this study show some of the individual glioma cell lines to have characteristic cell‐surface structures. They were found to be identical with the findings in previous SEM studies, suggesting the peculiarities of the individual malignant glioma lines to be stable and retained, despite continual pa

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00020.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Four hundred and eighty‐five primary malignant skin tumours submitted for histological diagnosis in Iceland during the period 1955–1974 were typed according to the WHO histological classification. The incidence of these tumours, age‐adjusted to the »World« population, was 12.6/100.000 in males and 11.0/100.000 in females. These are very low figures for a white population. The distribution by type among males was: basal cell carcinoma 66.8%. squamous cell carcinoma 22%, malignant melanoma 7.2% and others 4%. Among females: basal cell carcinoma 66.9%, malignant melanoma 20.8%, squamous cell carcinoma 10.2% and others 2.1%. The exposed areas of the body were affected much more frequently than other areas. The results support the theory that intensity of solar radiation is of major aetiological signi

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00021.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Lipogranulomas from severe fatty livers due to alcoholism, diabetes or overweight were examined in the electron microscope. When a fat droplet protrudes through the cell membrane of a liver cell histiocytes and lymphocytes settle around it, and a lipogranuloma is formed. Remnants of the liver cell may be seen between the fat droplet and the histiocytes. It is confirmed that the fat droplets are situated extracellularly in the fully developed lipogranulomas.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00022.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

In two simultaneously‐taken needle biopsies of the liver from 70 consecutive patients, a number of changes were evaluated semiquantitatively and compared in n × m tables. The lesions examined were steatosis, Mallory's hyalin, alcoholic hepatitis, cirrhosis and cholestasis; furthermore the size, amount of inflammatory cells, connective tissue and bile‐duct proliferation of the portal tracts and finally focal necroses and acidophilic bodies in the lobules were recorded. Cirrhosis, cholestasis and steatosis showed a high correlation, while the agreement between the two interdependent biopsies, especially concerning acidophilic bodies and bile‐duct proliferation, was low. Possible reasons for the variation in the agreement of the lesions are dis

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00023.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Bilateral bone‐marrow examinations from the posterior iliac crests were routinely performed as a pretreatment staging procedure in 89 consecutive patients with small‐cell anaplastic carcinoma of the lung. Bone‐marrow involvement was found in 20 patients (22.5%), in 9 (10.1%) only on one side, and in 11 (12.4%) on both sides. Aspiration was found to be significantly superior to biopsy, being positive in 25 examinations as compared with 14 positive biopsies. Compared with unilateral bonemarrow examination the positive findings increased by approximately 30%. Bilateral bone‐marrow examinations are recommended in patients in whom the detection of bone‐marrow metastases will have therapeutic imp

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00024.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Nuclear and cytoplasmic sizes of cells in permanent, stained smear preparations differ from those in unfixed unstained cells. In air‐dried MGG‐stained smears the area of the nucleus is 50% larger and that of the cytoplasm 30% larger. In wet‐fixed Papanicolaou‐stained smears the nucleus is 10–30% and the cytoplasm is 15–55% smaller. The shrinkage in the wet fixation method is dependent on the concentration of the ethyl alcohol applied. The staining method has relatively little influence on nuclear and cytoplasmic size. The three‐dimensional appearance of the smeared, stained cells is also dependent on the cytopreparatory technique applied: in the methods with air‐drying the nuclei and cells are flat and in the wet fixation method more spherical. In the methods with air‐drying the nuclear:cytoplasmic ratio is larger than that seen with the wet

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00025.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY