1. |
Detection of latent infection ofErwinia chrysanthemiandPseudomonas caryophylliin carnation1 |
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EPPO Bulletin,
Volume 16,
Issue 1,
1986,
Page 1-12
M. G. MURATORE,
U. MAZZUCCHI,
C. GASPERINI,
M. FIORI,
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摘要:
Three methods for detecting latent infections ofErwinia chrysanthemiandPseudomonas caryophylliin carnation stems were studied. The same procedure was used, in all three methods, to prepare the samples and to extract and concentrate bacteria. The samples consisted of 100–500, 1‐cm‐long, pieces of carnation stems. Under the standard procedure, the samples were homogenized in maceration fluid, then centrifuged at low speed to eliminate the larger tissue fragments and centrifuged again at high speed to obtain a final pellet with a maximum concentration of the bacteria to be detected. Immunofluorescence staining (IFAS), direct isolation (DI) and immunoenzymatic test (ELISA) were all applied to the final pellets. IFAS had a sensitivity threshold equal to 8.65 × 104and 3.33 × 104bacteria per 100 stem pieces forE. chrysanthemiandP. caryophylli.In simulated latently infected lots IFAS could detect down to 0.2% infected pieces for both pathogens, both at high and at low infection level. Although DI had a similar theoretical sensitivity threshold for both bacteria, it was less successful when applied to simulated latently infected lots. ELISA had the highest theoretical sensitivity threshold for both bacteria and as a consequence could only detect them in simulated latently infected lots with a high infection level. The comparative results are discussed in relation to the applicability of the methods to commercial lots of ca
ISSN:0250-8052
DOI:10.1111/j.1365-2338.1986.tb01126.x
出版商:Blackwell Publishing Ltd
年代:1986
数据来源: WILEY
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2. |
Xanthomonas campestrispv.corylina(Milleret al.1940) Dye 1978 |
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EPPO Bulletin,
Volume 16,
Issue 1,
1986,
Page 13-16
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ISSN:0250-8052
DOI:10.1111/j.1365-2338.1986.tb01127.x
出版商:Blackwell Publishing Ltd
年代:1986
数据来源: WILEY
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3. |
Xanthomonas fragariaeKennedy&King |
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EPPO Bulletin,
Volume 16,
Issue 1,
1986,
Page 17-20
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ISSN:0250-8052
DOI:10.1111/j.1365-2338.1986.tb01128.x
出版商:Blackwell Publishing Ltd
年代:1986
数据来源: WILEY
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4. |
Ceratocystis fimbriata(Ell. Halsted) f.sp.piliiatani(Walter) |
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EPPO Bulletin,
Volume 16,
Issue 1,
1986,
Page 21-24
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ISSN:0250-8052
DOI:10.1111/j.1365-2338.1986.tb01129.x
出版商:Blackwell Publishing Ltd
年代:1986
数据来源: WILEY
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5. |
Peach phony bacterium |
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EPPO Bulletin,
Volume 16,
Issue 1,
1986,
Page 25-27
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ISSN:0250-8052
DOI:10.1111/j.1365-2338.1986.tb01130.x
出版商:Blackwell Publishing Ltd
年代:1986
数据来源: WILEY
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6. |
Peach rosette mycoplasm |
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EPPO Bulletin,
Volume 16,
Issue 1,
1986,
Page 29-30
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ISSN:0250-8052
DOI:10.1111/j.1365-2338.1986.tb01131.x
出版商:Blackwell Publishing Ltd
年代:1986
数据来源: WILEY
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7. |
Peach yellows mycoplasm |
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EPPO Bulletin,
Volume 16,
Issue 1,
1986,
Page 31-33
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ISSN:0250-8052
DOI:10.1111/j.1365-2338.1986.tb01132.x
出版商:Blackwell Publishing Ltd
年代:1986
数据来源: WILEY
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8. |
Peach X disease mycoplasm |
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EPPO Bulletin,
Volume 16,
Issue 1,
1986,
Page 35-37
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ISSN:0250-8052
DOI:10.1111/j.1365-2338.1986.tb01133.x
出版商:Blackwell Publishing Ltd
年代:1986
数据来源: WILEY
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9. |
Pseudomonas syringaepv.persicae(Prunieret al.1970) Younget al.1978 |
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EPPO Bulletin,
Volume 16,
Issue 1,
1986,
Page 39-42
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ISSN:0250-8052
DOI:10.1111/j.1365-2338.1986.tb01134.x
出版商:Blackwell Publishing Ltd
年代:1986
数据来源: WILEY
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10. |
Apricot chlorotic leafroll mycoplasm |
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EPPO Bulletin,
Volume 16,
Issue 1,
1986,
Page 43-45
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ISSN:0250-8052
DOI:10.1111/j.1365-2338.1986.tb01135.x
出版商:Blackwell Publishing Ltd
年代:1986
数据来源: WILEY
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