摘要:

The present study describes a double antibody technique to evaluate antithyroglobulin autoantibodies in human serum. Rabbit anti-human γG is used to precipitate the immunocomplexes between labelled thyroglobulin and autoantibodies. Thyroglobulin has been labelled either in vivo, or chemically by electrolysis of iodide, a procedure which also produced substantial dissociation of the protein. The double antibody technique was compared with haemagglutination of sheep red blood cells coated with human thyroglobulin. A good correlation was established between the tanned red cell haemagglutination titre and the double antibody technique when ‘ in vivo’ labelled thyroglobulin was employed. The use of chemically iodinated thyroglobulin, purified after labelling, increased the sensitivity of the test 100 fold so that 38% of sera from patients with thyroid diseases having negative haemagglutination titres precipitate between 15% and 65% of the labelled antigen. The sensitivity and simplicity of this method provide a useful tool in clinical as well as in experimental

ISSN:1663-2818    

DOI:10.1159/000178252

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

The ability of leucine to stimulate insulin release was studied in an in vitro system, using microdissected pancreatic islets with a high proportion of the insulin-producing β-cells. When tested in a glucose-free medium, L-leucine stimulated insulin release over a wide concentration range with maximum effect at about 20 mM. The stimulation of insulin release was additive to that elicited by 10 mM glucose. The L-configuration was essential for leucine to be recognized by the β-cells as an insulin secretagogue. L-isoleucine, alone or in combination with L-leucine, did not affect insulin release. It seems, therefore, unlikely that all sites for L-leucine transport are identical with receptor sites for stimulation of insulin releas

ISSN:1663-2818    

DOI:10.1159/000178253

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

The insulin-releasing ability of some neutral and dibasic amino acids was studied in an in vitro system using micro dissected pancreatic islets with a high proportion of insulin-producing β-cells. The neutral amino acids L-alanine and α-aminoisobutyric acid had no substantial effects on basal and glucose stimulated insulin release. In contrast, 5–20 mM of the dibasic amino acid L-arginine gradually enhanced the insulin release induced by 10 mM glucose. A critical glucose concentration must be reached before L-arginine can act as an insulin secretagogue. N-methyl-L-arginine lacked the stimulatory ability. Co-transport of neutral amino acids and sodium does not elicit insulin release in adult mouse β-cells. There may, however, be a close relationship between the transport site for L-arginine and a receptor triggering insulin rel

ISSN:1663-2818    

DOI:10.1159/000178254

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

Present results show that ether anesthesia raises the mean plasma insulin levels and the variability of the individual data around the mean in rats under different experimental conditions. Previous results [6, 7] obtained in ether-anesthetized rats, showing that the plasma insulin levels of hypothyroid animals depends on the goitrogens used, and that there is a positive correlation between the individual thyroid weight and the plasma insulin levels, are confirmed in samples obtained without anesthesia.

ISSN:1663-2818    

DOI:10.1159/000178255

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

Hypothermia is seen in diabetic acidosis and its features may complicate the evaluation of the diabetic state. These include cardiac arrhythmias, low or normal temperature in the presence of infection, hypotension, depressed sensorium, moderate acidosis and possible resistance to the effects of insulin. Leukopenia may also be a feature of hypothermia in some of these patients. Speculation that hypothermia is dueto an intracellular lack of substrate for the production of heat in a number of diverse states is discussed.

ISSN:1663-2818    

DOI:10.1159/000178256

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

A review of the current state of knowledge and speculation on fluid absorption by the gastro-intestinal tract of vertebrates and more specifically the role of hormones in fluid movement has been made. The effect of hormones on water transport, active sodium transport and the electrical characteristics of poikilotherm vertebrate, bird and mammal intestine, both in vivo and in vitro are considered. Special reference is made to the effect of aldosterone on the absorption of fluid. Data indicate that while aldosterone has an effect on other tissues such as amphibian bladder, it appears to have only a slight effect on the jejunum and only on that component of the transport phenomena associated with the transfer of sodium.

ISSN:1663-2818    

DOI:10.1159/000178257

出版商:S. Karger AG    

年代:1972

数据来源: Karger