摘要:

Different parameters of the response to the 5-day metyrapone test were evaluated for their diagnostic accuracy in tests given to 58 children and adolescents as part of an investigation for short stature. Insulin test, vasopressin test and ACTH test were used for diagnostic reference. A log transformation of the data was clearly appropriate. The maximal daily excretion of 17-ketogenic steroids was distinctly positively correlated with the basal excretion. Consequently, the most correct parameter of the response is the SD score of the deviation of the maximal excretion from the regression of the maximal excretion on the basal excretion in the reference population. This parameter, ‘relative maximal excretion’ was shown to be the diagnostically most accurate index of the response of 11 parameters stud

ISSN:1663-2818    

DOI:10.1159/000178775

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

In primary aldosteronism due to an adrenal adenoma (n = 2), treatment with a spirolactone (160 mg Canrenone/day for 7 days) decreased plasma aldosterone and urinary aldosterone-18-glucuronide. However, in the presence of a normalization in urinary aldosterone-18-glucuronide plasma aldosterone remained elevated above normal. Continued therapy with higher doses (320 mg/day for 7 days and 480 mg/day for 28 days) did not significantly alter plasma aldosterone, while urinary aldosterone-18-glucuronide returned to values comparable to those obtained before therapy. Cessation of the drug resulted in a marked increase in plasma aldosterone and urinary aldosterone-18-glucuronide. The results indicate that in primary aldosteronism due to an adrenal adenoma, the spirolactone (Canrenone) inhibits aldosterone biosynthesis and seems to influence aldosterone degradation.

ISSN:1663-2818    

DOI:10.1159/000178776

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

Monomeric human growth hormone (hGH) and big hGH obtained by a new purification procedure from human pituitary glands were examined for a possible conversion into different molecular weight forms under in vitro and in vivo conditions. Sephadex G-150 gel filtration was used for separation. Big or monomeric hGH was incubated in hormone-free serum or injected into normal individuals in order to induce a peak of immunoreactive hGH. Specimens from early and late stages of the surge were subjected to gel filtration. It could be shown that not only the big hGH is converted to the smaller form but that monomeric hGH is also recovered in two higher molecular weight regions by gel filtration in vitro and in vivo experiments. A significantly higher conversion of monomeric to big hGH was found in vitro. This suggests an inhibition of big hGH formation from monomeric hGH in normal individuals. There was no apparent time relation of big hGH formation from monomeric hGH in vivo. Regarding the conversion of big hGH, more of this material remained in its original form in vitro. The immunoreactivity recovered in the region of the very large form was found to be higher in vivo. The formation of the monomer from big hGH was similar under in vitro and in vivo conditions.

ISSN:1663-2818    

DOI:10.1159/000178777

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

Iodinated bovine prolactin (2.6 iodine atoms/molecule; labelled with a trace of 125I to give a specific activity of 0.041 µCi/mg) was prepared by the chloramine T method. It was active in two bioassays (pigeon crop sac and dispersed mouse mammary cell), though somewhat less active than the unmodified hormone. In an immunoassay, iodinated prolactin was more effective than the unmodified hormone at displacing 125I-prolactin from antibody. High specific activity 125I-prolactin (1 iodine atom/molecule; 70 µCi/µg) was used for autoradiographic studies on the binding of prolactin to mouse mammary cells. In vivo the labelled hormone found in the mammary gland was associated with membranes of mammary epithelial cells and with alveolar lumen contents. In vitro 125I-prolactin was shown to bind to dispersed mouse mammary epithelial cel

ISSN:1663-2818    

DOI:10.1159/000178778

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

The effect of immobilization stress (IMO) on the plasma testosterone level in rats has been investigated. Plasma testosterone was not changed after 10 or 20 min IMO for 3 consecutive days as compared to nonstressed animals and the 10-min trial was without effect even if it was applied daily for 42 days. Three days IMO for 60 min caused a significant hormone decrease, which was even more pronounced after 3 or 42 days IMO for 120 min. The administration of HCG to rats immobilized 3 days for 120 min resulted in the increase of testosterone, however, the final level was significantly lower in comparison to nonstressed controls.

ISSN:1663-2818    

DOI:10.1159/000178779

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

The lipid composition of human testis in testicular and post-testicular causes of infertility was studied. The testicular causes of infertility was represented by patients with adult seminiferous tubule failure and Klinefelter’s syndrome. The post-testicular causes of infertility were represented by patients with obstruction of efferent ducts of testes. A significant accumulation of total testicular lipids was observed in both causes of infertility. A marked increase in free and esterified cholesterol, monoglycerides and triglycerides caused the accumulation of total testicular lipids in testicular causes of infertility. The accumulation of these lipids was more pronounced in Klinefelter’s syndrome compared to adult seminiferous tubule failure. In post-testicular causes of infertility, a very marked increase in phospholipids along with cholesterol fractions and glycerides led to the accumulation of total testicular lipids. The significant increase in total phospholipids caused by the increases in phosphatidylcholine and phosphatidylethanolamine differentiates post-testicular causes of infertility from testicular causes in which phospholipid classes remained unaffected. Thus, changes in hormonal mileu due to pathological conditions also lead to marked alterations in testicular lip

ISSN:1663-2818    

DOI:10.1159/000178780

出版商:S. Karger AG    

年代:1977

数据来源: Karger

ISSN:1663-2818    

DOI:10.1159/000178781

出版商:S. Karger AG    

年代:1977

数据来源: Karger