ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.t01-1-10103.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

摘要:

The cytokine macrophage colony‐stimulating factor was implanted in the rabbit cornea over a wide dose range (1 ng to 100 µg) to assay its angiogenic activity in vivo. Neovascularization occurred in a dose‐dependent manner, and maximum angiogenesis occurred only with 100 µg. Histologic analysis revealed that the corneas were free of inflammation at the lower doses, but had slight inflammation at 50 and 100 µg. Nonspecific esterase staining of frozen sections and transmission electron microscopy revealed that the inflammatory cells were predominantly macrophages, with very few neutrophils present. This association of capillary formation with inflammation suggests an indirect mechanism of angiogenesis. The lack of neutrophils within the inflammatory cell infiltrate demonstrates that indirect angiogenesis can proceed without the local presence of neutrophils. This distinguishes macrophage colony‐stimulating factor from other indirect‐acting angiogenesis factors that have been identifi

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10104.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10101.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10102.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

摘要:

Corticosteroids exert inhibitory effects on wound healing. They circulate, largely bound to corticosteroid‐binding globulin, and the plasma concentrations of this protein determine their bioavailability. The amount of corticosteroid‐binding globulin in wounds and the related effects of burn injury are not known. We have therefore measured corticosteroid‐binding globulin in serum and wound fluid obtained from subcutaneously implanted sponges, retrieved 1, 3, and 10 days after insertion in rats. The effect of burning was studied by comparing rats that had a small scald burn with sham‐burned control rats. In serum, corticosteroid‐binding globulin levels were lower in burned rats than in control animals: the difference was 22%, 28%, and 37% for days 1, 3, and 10, respectively (p<0.05 for each comparison), and values at day 1 were lower than at days 3 and 10 in control rats (p<0.05) but not in burned rats. In wound fluid, corticosteroid‐binding globulin levels were lower in burned rats than in control animals: the difference was 23%, 24%, and 34% for days 1, 3, and 10, respectively (p<0.01 for all comparisons), and the values were significantly higher (p<0.05) at day 1 when compared with values at day 10 in both groups. We therefore conclude that a small burn injury has significant effects on levels of corticosteroid‐binding globulin on serum and wound fluid corticosteroid‐binding globulin. The decreased concentration of wound fluid corticosteroid‐binding globulin at day 10 versus day 1, with a concomitant increase in serum corticosteroid‐binding globulin, suggests an accelerated degradation of the protein within the wound; this phenomenon is exaggerated by the burn injury. This is supported by Western blot analysis, which revealed the appearance of a small polypeptide that reacts with an antiserum against rat corticosteroid‐binding globulin in

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10105.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

摘要:

Fetal rabbit wounds that are sutured show excellent repair without obvious scarring. In contrast, an unsutured wound in a rabbit fetus does not close, and it appears that the process of wound contraction does not occur. Experiments were carried out to illustrate the mechanisms responsible for the noncontraction of open fetal rabbit wounds. Results showed that the lack of wound contraction was not an artifact caused by rapid fetal growth. With regard to the ability of cultured fetal fibroblasts to show cytoplasmic muscle‐induced cell contraction, we found that, in cultured fetal fibroblasts, cell contraction was induced by adenosine triphosphate. Contractile abilities of fetal‐derived fibroblasts were equivalent to those of adult‐derived fibroblasts. The fetal fibroblasts also demonstrated the generation of superior contractile activity when examined in a fibroblast‐populated collagen lattice model. Finally, the ability of amniotic fluid to alter wound contraction was addressed by means of the fibroblast‐populated collagen lattice in vitro model. Increasing concentrations of amniotic fluid inhibited fetal fibroblast lattice contraction. Therefore, rabbit amniotic fluid contains an inhibitor that may be partially responsible for the noncontraction of fetal rabbit wounds

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10106.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

摘要:

Increased fibroblast activity and collagen production have been observed frequently in proliferative scars. Previous studies have demonstrated that interferons suppress collagen production by means of normal, keloid, and hypertrophic scar‐derived fibroblasts. The fibroblast‐populated collagen lattice is an in vitro model used to study fibroblast function. We used fibroblast‐populated collagen lattices to evaluate the effect of interferon on fibroblasts harvested from normal human skin, human keloid, and hypertrophic scar tissues. Human recombinant interferon alfa‐2b (1000 IU/ml) was added to the culture media. The collagen gel, prepared from rat tail tendon bundles, was overlaid with 5 × 104fibroblast cells. Keloid fibroblast‐populated collagen lattices showed the highest contraction. Contraction in all the groups appeared suppressed by interferon alfa‐2b during the first 72 hours of study (p<0.05). The reduction in fibroblast‐populated collagen lattice contraction by interferon alfa‐2b was similar among the groups. The contractile properties of fibroblasts taken from normal human skin, keloids, and hypertrophic scars in this in vitro study were suppressed by interferon alfa‐2b. This suggested that interferon alfa‐2b may be beneficial for the treatment of

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10107.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

摘要:

The presence and cellular distribution of epidermal growth factor (EGF), TGF‐α, and EGF‐R were determined in the rat fascial and peritoneal tissue during healing of an incisional injury by means of immunohistochemistry and autoradiographic techniques. The immunostaining intensity for EGF in the regenerating wound area was substantially higher during the first 14 days, then decreased to near prewound levels during 14 to 35 days after surgery. Within the wound area, the most intense immunostaining occurred with inflammatory cells, followed by fascial striated muscle and arterioles, whereas fibroblasts in the regenerating area contained very low immunostaining intensity. The immunostaining pattern for TGF‐α with the use of three separate polyclonal antibodies that were directed against the amino and carboxy termini of TGF‐α precursor and a fragment of the mature 50‐amino‐acid form of TGF‐α was similar to that seen with EGF and persisted until 28 days after injury. However, fibroblasts in the regenerating area immunostained intensely for TGF‐α but not for EGF. Quantitative autoradiography of iodine 125—labeled EGF binding and immunohistochemical studies of the EGF‐R with monoclonal antibodies that were directed against the extracellular binding domain of EGF‐R demonstrated the presence of specific EGF‐R in regenerating fascial and peritoneal tissue. Net grain density (100 µm2), representing specific binding of125I‐EGF, was calculated for different cell types in the wound. The grain density over fascial striated muscle, migratory fibroblasts and peritoneal fibroblasts increased by two and one half, three, and four times, respectively, at 7 days and decreased to the values in adjacent unwounded tissue by 21 days after injury (p<0.05). Immunostaining for the EGF‐R generated similar patterns, which persisted for 14 days after injury. The grain density and immunostaining for EGF‐R over the arterioles in the wound did not change during the course of healing and was similar to that of the uninjured regions. In summary, these observations indicate that the local levels of EGF, TGF‐α, and EGF‐R increase during the early phases of healing in fascial and peritoneal injury, which suggests a role for these growth factors in the normal mechanism of fascial/peritoneal woun

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10108.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

摘要:

The purpose of this study was to assess and evaluate the role of transforming growth factor‐β1, epidermal growth factor, and their respective carriers (collagen and liposomes) in the early phases of the wound‐healing process in linear incision wounds; we used an in vivo biomechanical testing system. One hundred twenty specific pathogen—free male Sprague‐Dawley rats were divided into five experimental groups (n = 24), including one group receiving no treatment at all. Each animal received one abdominal midline incision. At 3 and 5 days after wounding, 12 animals per group were randomly selected for in vivo biomechanical evaluation. Specimens were also randomly obtained from nondisrupted tissues for histologic analysis. Statistical analysis comparing groups revealed that transforming growth factor‐β1significantly increased wound strength at day 5, and liposomes decreased wound strength at day 3. There were no other significant differences among groups for each of the time intervals studied. Our results suggest that in vivo biomechanical evaluation of tissue response to injury and treatments will add new dimension to future studies of skin and wo

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10109.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY

ISSN:1067-1927    

DOI:10.1046/j.1524-475X.1993.10110.x

出版商:Blackwell Science    

年代:1993

数据来源: WILEY