摘要:

A non‐lysogenic strain ofPseudomonas aeruginosamaintained its phage sensitivity pattern and serogroup specificity unchanged for 10 weeks in ex‐germfree, mono‐contaminated rats before infection with phage. After infection with phage, phage conversions of serogroup specificity and lysotype were observed. With the same combination of bacterial and phage strains, the same serogroup was obtainedin vitroandin vivo. In vitroconversion occurred also to serogroups that were not detectedin vivo.Upon lyophilization, converted bacterial clones from thein vivoexperiment lost their phage and simultaneously reverted to the original phage type and serogroup. These findings may have implications for the understanding of the degree of stability in epidemiological typing results forP. aerug

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00062.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Two groups of patients, each consisting of 40, were investigated for the occurrence of mycoplasmas in the urethra, bladder and upper urinary tract. Mycoplasmas were isolated significantly more frequently from the bladder urine of patients with chronic pyelonephritis than from patients with non‐infectious urinary tract diseases. Furthermore, mycoplasmas were isolated from the upper urinary tract of 5 patients with chronic pyelonephritis, while mycoplasmas could not be cultivated from the upper urinary tract of patients with non‐infectious urinary tract die

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00063.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Salmonella bacteria were stained with serial dilutions of anti‐Salmonella conjugates of different F/P ratios and the staining intensity was measured quantitatively in an ultramicrofluorometer. In any given dilution of the conjugates, a stronger fluorescence was obtained with the more highly labelled conjugates. The dependence of fluorescence intensity on F/P ratio varied with the dilution of the conjugate. Similar results were obtained by the indirect immunofluorescence method. In four bacterial systems the direct and indirect immunofluorescent staining methods were compared quantitatively. The indirect method was 5 to 30 times more sensitive than the direct comparing the last dilutions giving a positive reaction by visual observations. The standard deviation of the intensity values of the stained bacterial cells was between 10 and 40 per cent of the mean. Different sources of variation in the quantitative measurement technique are discusse

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00064.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

1) Eighty‐seven breast‐fed and 95 bottle‐fed infants were examined for differences inEscherichia coliserogroup pattern. 2) The serogroup composition was less complex in breast‐fed infants. Fewer serogroups were found in the individual breast‐fed child. However, with few exceptions, the same O groups were found in the two groups of infants. The prevalent O groups were among those also found most frequently in faeces from adults. 06 strains were more common in the breast‐fed group, while 07 and 016 were not at all found in that group. 3) A special search forE. colistrains with K1 antigen showed that this antigen predominated in the bottle‐fed infants. 4)Klebsiellaand otherEnterobacteriaceaewere more frequently found among bottle‐fed infants. 5) It is concluded that the intestinal milieu exerts a selective pressure which may result in a selection of different serotypes in two different intestinal milieus such as

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00065.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

The transmissible antigen in enteropathogenicE. colistrains from calf and lamb, previously called Kco, is established as theE. coliK99 antigen. It is probably of protein nature since it is destroyed by heating. It is pointed out that other antigens present, growth medium and unknown factors are of great importance for the demonstration of this antigen.

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00066.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

By means of radio‐isotope technique, the influence of methylprednisolone treatment on opsonic and bactericidal serum activities againstE. coliwas studied on germfree and monocontaminated rats. Based on the present results it seems unlikely that methylprednisolone impairs the production of complement components. A slight suppression of antibody activity was only found in markedly wasted monocontaminated rats treated with very high doses of methylprednisolone from the day before contaminatio

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00067.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Highly active antigen preparations, as measured by precipitation and inhibition of haemag‐glutination, were obtained fromBacteroides fragilisNCTC 9343 grown in continuous culture. The activity varied slightly only with the glucose concentration of the medium and the dilution rate. pH had a greater influence on antigen productio

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00068.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Herpes simplex virus (HSV) type 1 and type 2 specific immune sera have been produced by adsorption with glutaraldehyde‐polymerized heterologous virus antigen. The adsorbed sera showed high specificity in complement fixation, immunoelectroosmophoresis and neutralization tests. The precipitin patterns in IEOP indicate the presence of at least five HSV type 1 and three HSV type 2 specific antigen

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00069.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Determination of the concentration of each drug in the combination trimethoprim: sulphamethoxazole was performed in patient sera by separation of the drugs by electrophoresis in agarose‐gel followed by microbiological assay. The electrophoretic method was equivalent to the methods used in the routine and showed good reproducibilit

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00070.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

The pathogenicity for rat ofM. arthritidisstrain Jasmin and strain 20‐P from rheumatoid synovial tissue were studied. Only strain Jasmin produced signs of illness in four of the 12 rats infected. A mycoplasma was isolated from three tissue specimens. Ten of the 48 rats investigated showed mycoplasma antibodies. Five rats showed minor inflammatory changes in the distal joints of the limbs. The failure to induce arthritic symptoms in rats inoculated with strain 20‐P from rheumatoid arthritis may be due to its 10‐year long storage outside the animal

ISSN:0304-131X    

DOI:10.1111/j.1699-0463.1975.tb00071.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY