摘要:

When grown with maltose or starch as carbon source at 37°C,Bacillus licheniformissynthesized two α‐glucosidases which could be resolved by polyacrylamide gel electrophoresis. One of these enzymes preferentially hydrolysedp‐nitrophenyl‐α‐D‐glucoside (pNPG) and the other was inactive onpNPG but hydrolysed maltose (maltase). Only the maltase could be detected in induced cells g

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1987.tb01568.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Laser densitometer scanning and three methods of computation were investigated for estimating sizes of restriction endonuclease digest fragments ofCampylobacter jejunichromosomal DNA. Accuracy of the procedures was determined with standard bacteriophage lambda DNA fragments. The program DNASIZE was the most accurate for fragments between 2.3 and 33.5 kb while the program DNAFRAG and multiple regression analysis gave slightly greater errors particularly for smaller fragments.HaeIII fingerprints of DNA from sixC. jejunistrains contained at least 25 resolvable fragments with sizes between 3.2 and 35.8 kb estimated by DNASIZE and several differences between pattern types were detected.

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1987.tb01569.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

A revised method is described for the application of the API 50 CH carbohydrate kit to yeasts. The method permits an objective assessment of positive assimilation with a high degree of reproducibility.

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1987.tb01570.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The heat resistance ofSalmonella typhimurium, measured as survival following a standard heat challenge at 55°C for 25 min, increased progressively as cells were heated up at linearly rising temperatures. The amount by which heat resistance increased depended on the rate of temperature rise; the slower the temperature rise, the greater the increase in resistance

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1987.tb01571.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

A short scheme for the identification of thoseviridansstreptococci commonly isolated from the human mouth was produced by selecting the most discriminating tests from the study made by Facklam (1977). The scheme comprised tests for the fermentation of mannitol, sorbitol, inulin, raffinose, melibiose and trehalose; the hydrolysis of arginine and aesculin and the production of dextran or levan from sucrose. The scheme was cost‐effective in terms of preparation time and materials and was shown to reliably identify over 86% of the streptococci strains used in the stud

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1987.tb01572.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

A simple method for detecting heparinase‐producing bacteria is described. The method is based on the metachromatic reaction between heparin and toluidine blue. Though developed primarily forBacteroidesspp., the method should find application in the detection of other aerobic and anaerobic heparinase‐producing bacteria, without the need for large amounts of media and expensive spectrophotometric equipm

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1987.tb01573.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY