摘要:

During hydrolysis of protein‐extracted lucerne fibre byTrichoderma reeseicellulases, the enzymes rapidly adsorbed to the substrate and were slowly released as the carbohydrate was solubilized. Comparison of cellulase component fractionation patterns on ion exchange chromatography revealed differences between the unreacted enzymes and those recovered after desorption. Although total cellulase activities were about equivalent, there was a four‐fold shift in the ratio of cellulase components after the adsorption/desorption cy

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01201.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The control of a thermophilic continuous anaerobic digestion system when subjected to potential inhibitory shock loadings was achieved through the regulation of dissolved H2, monitored using membrane inlet mass spectrometry, by the controlled addition of carbon source. At a feed pump switching threshold equivalent to 1 μmol/1 H2a steady state rate of methanogenesis of approximately 40 μmol/1/min was obtained. Higher H2thresholds resulted in an inhibition of methanogenesis, but precise control of H2concentration was demonstrated with an oscillatory response of period 2·5–5·

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01202.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

A DNA fragment containing a Klebsiella oxytoca gene for aesculinase activity was cloned on the multicopy plasmid pBR322. This β‐glucosidase activity was confined to aesculin hydrolysis only. It was expressed equally well inEscherichia coli, Salmonella typhimuriumandAeromonas hydrophila.Two polypeptides were found to be encoded within the 2·6 kb of the cloned DNA encoding aesculinase activ

ISSN:0266-8254    

DOI:10.1111/j.1472-765X.1988.tb01203.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY