摘要:

AbstractPatients with the acquired immunodeficiency syndrome (AIDS) are susceptible to a variety of opportunistic pathogens which require intact cellular immunity for control and eradication. We evaluated interleukin 1 and 2 production in 12 homosexual men without AIDS but with evidence of altered cell-mediated immunity and serologic evidence of infection with human T-cell lymphotrophic virus type III (HTLV-III). the etiologic agent of AIDS and found production of both factors diminished compared to heterosexual controls. Therafectin (SM-1213) is a new agent which selectively activates macrophages and stimulated interleukin 1 productionin vitro.Therafectin was administered to these same 12 patients in a double-blind, placebo controlled trial. We failed to find any significant changes in their immunologic status including interleukin 1 or 2 production.

ISSN:0892-3973    

DOI:10.3109/08923978609031082

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractBy altering the receptor binding specificity of the highly potent natural toxin ricin, a macrophage specific immunotoxin was developed. Ricin ordinarily does not demonstrate cell type specificity and is capable of binding and entering cells through galactose containing receptors resulting in rapid cell death. A murine anti-rat peritoneal macrophage IgG1 monoclonal antibody, B-6, was developed to serve as a target specific carrier for ricin. By covalently binding monoclonal antibody B-6 and reversibly binding lactose to ricin, a new biologically active hybrid toxin possessing macrophage specificity was developed. When P3X63-Ag8.653 myeloma cells, which served as an nonspecific target cell type, and macrophages were treated with the ricin conjugate over a broad range of concentrations and various time periods, the conjugate demonstrated substantially greater toxicity toward macrophages than myeloma cells even though both cell types responded similarly to treatments with unconjugated ricin. It was also observed that ricin was considerably more toxic to macrophages when conjugated to monoclonal antibody B-6 than unconjugated ricin. Through ricin-antibody conjugation a high degree of specificity and toxicity can be attained potentially suitable for anti-tumor reagents and immunomodulators.

ISSN:0892-3973    

DOI:10.3109/08923978609031083

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractA definite increase in two low molecular weight factors, G10-2 and G10-3 was found in Ehrlich ascitic fluids, parallel to tumor growth. The isolation and identification of the two factors were attempted through gel filtration and reversed phase column chromatography, using ascitic fluids obtained 13 days after intraperitoneal implantation of Ehrlich tumor cells. As a result, two highly purified factors were observed upon examination by high performance liquid chromatography. Additional analytical data, collected by UV spectrum, NMR spectrum and mass analysis. allowed us to identify G10-2 as uric acid and G10-3 as uracil.Detailed immunological analysis of uric acid and uracil revealed that the augmenting activities of mouse and human NK cells by mouse IFNδ/βor human rIFNδA/D were impaired in the presence of either compound at concentrations of 0.07 mM, the concentration detectable in theascitic fluidof tumor bearing mice.

ISSN:0892-3973    

DOI:10.3109/08923978609031084

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractThe low molecular weight fraction (mol wt<1,000) of Ehrlich cancer ascitic fluid has been known to enhanceListeriainfection in mice. Chemical characterization of the entities in this fraction revealed four purine and pyrimidine analogues, i.e. uric acid, uracil, pseudouridine and 1-methyladenosine (m1Ado). When the effect of each of these components was studied onListeriainfection in mice, only m1Ado markedly enhanced the infection and killed the mice within a short period. The optimal enhancement was obtained when m1Ado was given intravenously to mice 3-6 days before the infection at a concentration of between 1 and 100μg/mouse. On the other hand, uric acid, uracil and pseudouridine failed to show such an enhancing effect. m1Ado inhibited macrophage accumulation in the peritoneal cavity of mice after an intraperitoneal injection of phytohemagglutinin. Although m1Ado did not show any inhibitory effect on the phagocytic and bactericidal activities of macrophagesin vitro.

ISSN:0892-3973    

DOI:10.3109/08923978609031085

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractA variety of cellular immune responses involve complement factors which bind to specific receptors, and modulate or effect a specific reaction. Monoclonal antibodies (MAb) have been generated against complement receptors (CR) 1 and 3, which were utilized to investigate human allogeneic and Epstein-Barr virus specific cytotoxic cells in vitro. MAb OKM1, which binds to the C3bi CR (CR3), and MAb M710, which binds to the C3b/4b CR (CR1), inhibited the generation of both allogeneic and virus specific cytotoxic responses in vitro in a dose-dependent way; doses of 1μg/ml (or greater) completely abrogated the cytotoxic responses. Inibition of these responses was observed when the MAb was added to the cultures at any time point except the last two days. In addition, treatment of the responder (but not the stimulator cells) with either MAb resulted in complete inhibition of cytotoxic responses. These experiment indicate that complement receptors participate in the generation of human cytotoxic responses in vitro.

ISSN:0892-3973    

DOI:10.3109/08923978609031086

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractThein vivoadministration of tumor necrosis factor (TNF) provides a new approach to the immunotherapeutic treatment of tumors. We evaluated the pharmacokinetics of murine tumor necrosis factor in mice as a model for application in the human system. TNF had a clearance of 0.013 ml/min/g and a serum half life of 10.5 minutes. Its volume of distribution was consistant with the extracellular space. This information can provide parameters by which to select optimal modes of treatment for eradication ofin vivoneoplasms.

ISSN:0892-3973    

DOI:10.3109/08923978609031087

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractThe effect of multiple sublethal doses of busulfan on the haemopoietic system of the Balb/c mouse was examined. FBC's bone marrow histology and bone marrow progenitor cell assays (CFU-E, BFU-E, CFU-GM and CFU-GEM) were performed. No effect was seen on FBC's or on marrow histology. However there was a significant depletion noted in the number of haemopoietic progenitor cells as measured by in vitro culture.

ISSN:0892-3973    

DOI:10.3109/08923978609031088

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractThis phase I study demonstrates the immunostimulating properties of SL04 administered by the oral route in the healthy volunteer. Responses to the test of DTH (delayed type hypersensitivity) were enhanced by SL04 and the production of MIF (migration inhibiting factor) involved in this response was stimulated. These properties could be correlated with the previously demonstrated induction of interferon by SL04.

ISSN:0892-3973    

DOI:10.3109/08923978609031089

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor