ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00203.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:A new column chromatography technique, utilizing peroxidase‐labeled antihuman immunoglobulins, is described, which can measure the binding of specific antibodies to membrane fragments containing relevant antigens. Using this assay, the presence of bound immunoglobulin is demonstrated on membrane fragments isolated from ascites fluids, but not from tumor tissues. In this study, two populations of immunoglobulins were examined: ‘free’ immunoglobulins and immunoglobulins bound to ascites fluid‐derived membrane fragments. Immunoglobulins eluted from membrane fragments of the ascites fluids were capable of binding to the tumor. These were specific for binding to tumor membrane preparations, in that there was two orders of magnitude greater binding of these immunoglobulins at a given concentration than ‘free’ immunoglobulins at the same co

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00204.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00205.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:Titers of hemagglutination and cytotoxic antibodies to sperm were determined in the sera, seminal plasma, cervical mucus, and vaginal secretions from 69 infertile couples, using sperm from the husbands and from normal control subjects. Titers of hemagglutination and cytotoxic antibodies were significantly higher (p = 0.002 and p<0.001, respectively) against autologous sperm as contrasted with sperm from control subjects in sera from 55 autoimmune males. Hemagglutination and cytotoxic antibody titers were also significantly higher (p = 0.044 and p<0.001, respectively) against their husbands' sperm as contrasted with control sperm in sera from 46 females with isoimmunity to sperm. Ninety percent of males and females with sperm immunity were positive for histocompatibility antigens HLA‐B7, HLA‐B8, and/or HLA‐BW35. In males, the presence of increased serum cytotoxic antibody titers against autologous versus control sperm was significantly associated with the presence of HLA‐B7 allele (p = 0.0017) and with HLA‐B7, HLA‐B8, and/or HLA‐BW35 in general (p<0.05). In the females, increased serum antibody titers to their own husbands' versus control sperm were not preferentially associated with HLA‐B7, HLA‐B8, or

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00206.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:Human seminal fluid, at low dilutions, prevented the binding of aggregated human IgG (AHG) to bull spermatozoa. Seminal fluids from vasectomized men were also inhibitory. Preincubation of the seminal fluid with the spermatozoa prior to washing and addition to AHG had no inhibitory effect, indicating that the fluid component was reacting directly with AHG. Human seminal fluid was fractionated by gel exclusion chromatography on Ultrogel AcA‐34, and AHG inhibitory activity was found in fractions corresponding to a molecular weight of 94,000. The activity in this fraction was stable to boiling for 10 min. It was sensitive to pronase but resistant to glycosidase, phospholipase C, neuraminidase, ribonuclease, and deoxyribonuclease, indicating that it was a protein. The gel filtration fraction readily bound recrystallized Fc and AHG; IgG was bound to a lesser extent, and no reactivity was observed with F(ab')2, IgA, or IgM. Thus, the seminal fluid fraction appeared to specifically react with the Fc portion of IgG. The seminal fluid Fc‐binding protein was isolated by affinity chromatography on Fc coupled to CNBr‐activated Sepharose 4B. Scatchard analysis revealed that the binding of the seminal fluid Fc‐binding protein to recrystallized Fc is reversible and had a Kd of approximately 3

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00207.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:Antisperm antibodies have been measured in serum and in seminal plasma in 130 males before and after vasectomy reversal, and the occurrence of pregnancy has been analyzed in those partners who were trying to produce a pregnancy. All patients have been followed for at least one year. Sperm‐agglutinating antibodies were found in the serum of 79% of patients; seminal plasma antibodies were present in only 9.5% before reversal, and this rose to 29.5% afterwards. Overall, pregnancies occurred in the partners of 44.6% of those men who were trying to produce children. Production of pregnancy was significantly less likely when the preoperative serum antisperm antibody titer was 512 or more, but no decrease in fertility was seen with titers below this. Similar numbers of pregnancies were produced by patients with or without seminal plasma antibodies in titers of up to 16; there are too few patients with titers above this level to permit further analysis. A randomized controlled trial of perioperative steroids showed that they produced no benefit. It appears that the antisperm antibodies associated with vasectomy reversal may differ fundamentally from those occurring in naturally subfertile male

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00208.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:In view of the importance of the syncytiotrophoblast in the immunobiology of human pregnancy, the composition of this interface between fetus and mother was further studied by a combination of physicochemical and immunological approaches. Trophoblast membranes were solubilized using three classes of detergents: zwitterionic (sulfobetaine14), non‐ionic (Triton X‐100), and anionic (deoxycholate). Quantitative studies of protein released demonstrated the importance of dispersion of membranes into detergent, and optimum solubilization was then obtained at detergent:protein ratios of 1.3:1 for sulfobetaine, 2.5:1 for Triton X‐100, and 4.4:1 for deoxycholate. Analysis of chaotrope‐treated detergent‐solubilized membrane components was performed by preparative isoelectric focusing followed by combined physicochemical and immunological methods. The results revealed three major proteins which were identified as placental alkaline phosphatase, albumin, and transferrin. Certain additional low molecular weight proteins were also evident; one of these components displayed physicochemical properties similar to those of actin, but none appeared to be recognized by conventional heteroantisera raised to trophoblast membrane. These findings and the results of further immunological analysis by affinity chromatography are consistent with the concept that human trophoblast may express limited immunogenicity due in part to the presence of large amounts of absorbed maternal serum components and limited expression of fetal

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00209.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

ABSTRACT:The titer, immunoglobulin class, and staining pattern of smooth muscle antibodies (SMA) were assayed by indirect immunofluorescence in 15 patients with threatened abortion, 46 patients with spontaneous abortion, and 45 normal pregnant women in the first trimester to investigate possible abnormality of humoral immunity. An increased incidence of moderate and high titer SMA was found in both abnormal groups. SMA were mainly of the IgG class and gave a “vessel wall” staining pattern in immunofluorescence. This staining pattern suggests non‐actin specificity of SMA. The more precise characterization of the antigen specificity of SMA in complications during pregnancy requires further investig

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00210.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00211.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1983.tb00212.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY