ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1981.tb00015.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

ABSTRACT:Disagreement as to whether Ig levels are high, low, or normal at different ages in different groups of trisomic children has interfered with defining the associated immunodeficiency state. Fetal Ig production was assessed by measuring cord blood IgM and IgA of five trisomy 21, 97 normal, and 37 control newborns with other birth defects. Trisomic infants showed significantly lower values. Cord blood IgG values showed no differences between groups when corrected for fetal birth weight. The data indicate that children with trisomy 21 have an impaired humoral immune response in utero.

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1981.tb00016.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1981.tb00017.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

ABSTRACT:The development of immunocompetence during gestation depends upon the sequential differentiation of antigen‐specific lymphoid cells in the context of epithelial inducing microenvironments. These early intrauterine events, which appear to be antigen‐independent, include clonal diversification of idiotypes and isotypes as well as commitment to B or T cell lineages. The steps in cellular maturation can be traced through the use of lymphocyte differentiation markers. Cooperation among lymphoid subsets, as well as from nonlymphoid cells and possibly other cofactors, is necessary for the effective function of this array of lymphocytes. The rate of expansion of functional immunity may be limited as much by the ontogeny of these collaborating influences as by the intrinsic immaturity of the B and T cells themsel

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1981.tb00018.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

ABSTRACT:Efforts were made to disrupt and solubilize human sperm cells and to evaluate the product for its content of infertility antibody‐related antigens. In the procedure that was developed, a well‐washed sperm sample is treated with 0.1 M dithiothreitol for 60 min, followed by trypsin at 0.1 mg/ml for 30 min, and then by soybean trypsin inhibitor. A mixture of DNAses I and II are added. After centrifuging, the resuspended pellet (RP) and the final supernatant (FS) show several degrees of cellular breakdown. Immunological evaluation was done with a strongly positive human serum containing sperm‐head antibody. From the inhibition of sperm agglutination, we could conclude that the desired soluble antigen was obtained in the FS fra

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1981.tb00019.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

ABSTRACT:The responses of spleen cells from male and female BALB/c mice were evaluated to determine if sex‐related variations in immune expression could be found. The immunologic assays used included blastogenic responses to mitogens, and direct and indirect measurement of plaque‐forming cells against particulate antigens. The results indicated that responses of spleen cells from young adult female mice were higher than those of males in all comparative tests. Newborn mice did not demonstrate the sex‐associated immune differences; and among the weanling mice slight differences between male and female spleen cells responsiveness to mitogenic agents were observed.The blastogenic responsiveness of spleens from female BALB/c was greater at proestrus and metestrus, as compared to estrus and diestrus. The peaks of responsiveness corresponded to reported elevated levels of estrogen and pregnenolone during these phases of the cycle. Similar results were obtained with the IgM plaque‐forming cell responses, which were also increased at proestrus and metestrus. This study supports a role of sex hormones in modulation of immune exp

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1981.tb00020.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

ABSTRACT:Immune reactivity of primiparous pregnant C57B1/6J mice was investigated using in vitro assays of mitogen reactivity. The response to the T cell mitogens phytohemagglutinin (PHA) and concanavalin A of cells from the paraaortic (PA) lymph nodes, which drain the uterus, was decreased in pregnant animals. Reactivity to lipopolysaccharide, a B cell mitogen, was normal. The decreased PHA response was seen with PA cells from mice bearing syngeneic or allogeneic (to DBA/2J) fetuses. It was not due to a change in sensitivity to PHA dose or to active suppression (as demonstrated by mixing experiments).Phytohemagglutinin reactivity of cells from inguinal nodes of pregnant mice showed a more variable depression of response in comparison to that seen with cells from the draining PA nodes. The response of axillary and brachial node cells was similar to virgin values.This study demonstrates that pregnant mice (syngeneic or allogeneic) show only a decrease in T proliferative capacity localized to the area of the uterus Statistical analysis revealed no differences in the average number of PA lymphocytes or fetuses per mouse between mice bearing syngeneic or allogeneic fetuses. This parallels the similarities found between syngeneic and allogeneic matings in in vitro functional assays.

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1981.tb00021.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

ABSTRACT:Further data are presented on the isolation and biochemical analysis of syncytiotrophoblast microvillus plasma membrane preparations from normal full‐term human placentas. No evidence has been obtained that IgG associated with these membranes is maternal antibody to trophoblast antigen. An oncotrophoblast plasma membrane antigen was previously identified by immunofluorescence, and an indirect radioisotopic antibody assay has now been used to confirm the detection of a cell surface antigen common to breast carcinoma cells and normal trophoblast. The relation of trophoblast plasma membrane antigens to possible maternal immunization in pregnancy is discusse

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1981.tb00022.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

ABSTRACT:The addition of autologous serum to mixtures containing human red cells, from pregnant and nonpregnant females, and sheep red cells resulted in the formation of mixed aggregates containing both human and sheep red cells. In contrast, no aggregate formation occurred when autologous cord serum was addded to mixtures containing cord red cells and sheep red cells. Heat inactivation of the adult serum or the presence of 0.15 M EDTA prevented the formation of mixed aggregates. These observations indicated that the mixed aggregates occurred through the complement‐dependent red cell immune adherence (RCIA) phenomenon. The addition of untreated cord serum to mixtures containing inactivated adult serum restored the formation of mixed aggregates, indicating that the cord serum contained sufficient complement for RCIA. Natural antibody against sheep red cells was present in adult sera but was absent in cord sera.Using the RCIA receptor assay, the RCIA receptor activity of cord red cells was found to exceed significantly that of the adult pregnant cells (p<0.0025). It is postulated that this may represent an aspect of immune adaptation between mother and fetu

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1981.tb00023.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1981.tb00024.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY