ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00075.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:Previous studies from our laboratory using vasectomized rats of different inbred strains have suggested that MHC‐linked genes do not play a major role in the formation of autoantibodies to spermatozoa. In the present paper, we demonstrate that sperm autoantibody production is not influenced by genes in the major histocompatibility complex, using vasectomized congenic rats and various crosses between high and low responder strains. We also show that autoimmune responses to spermatozoa are not influenced by genes on the Y‐chromo

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00076.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:Circulating immune complexes were detected in women on oral contraceptives (OC) by a simple antigen nonspecific method using precipitation of serum in 25% saturated ammonium sulfate (CIC‐AS). A significant correlation was found between the presence of CIC‐AS and the OC vascular risk.A radioimmunoassay with tritiated ethinylestradiol indicated that CIC‐AS contained antiethinylestradiol antibodies (anti‐EE Ab) in a number of OC users, but indicated also that 1) anti‐EE Ab may be found in cases with no detectable CIC‐AS, 2) CIC‐AS containing no anti‐EE Ab are found in nonusers, and 3) even in OC users the CIC‐AS may contain antibodies to other ligands than EE.The study demonstrated also that, in OC users with a vascular complication, anti‐EE Ab were more frequently detected (78% of cases) than CIC‐AS (60%). Moreover, among OC users with CIC‐AS, anti‐EE Ab were found in 95% of women with a vascular complication and only 37% of current healthy users.The detection of anti‐EE Ab appears to be more predictive, with regard to the vascular risk of OC, t

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00077.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:The immunoglobulin classes of the sperm‐agglutinating antibodies in the seminal fluid and serum from four vasovasostomized men, as well as in operatively obtained testis‐epididymis fluid from two of them, were investigated by specific solid‐phase absorption of IgG, IgM, and IgA. In two patients the major part of the seminal agglutinins was IgA, and in one IgG, indicating that both local production and diffusion of sperm agglutinins from serum to the testis‐epididymis compartment may take place. The total levels of immunoglobulins in seminal fluid generally increased after the operation, and IgM was present in two postvasovasostomy samples. This suggests a pathotopic potentiation effect of the vasectomy on the epithelial lining of the testis‐epididymis compartment. Comparison of the results of solid‐phase absorption with anti‐IgA and antisecretory component (SO pointed to the presence of “free” SC in postvasovasost

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00078.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:Endometrial lymphocytes were isolated in two groups of fertile women, 17 of them normal and seven with various clinical problems. In order to study their immunological properties, endometrial and blood lymphocytes were cultured with either phytohemagglutinin (PHA), concanavalin A (Con A), pokeweed (PW), or purified protein derivative (PPD). Lymphocyte response was assayed by tritiated thymidine incorporation. The percent of T cells was estimated by E‐rosette formation. In the normal fertile group, endometrial lymphocyte response to PHA and to Con A was found to correlate with the days of menstrual cycle, rising in a linear fashion between days 9 and 24. In the group with clinical problems, no correlation was noted. In neither group were endometrial lymphocyte responses to PW or PPD or percent of endometrial lymphocyte rosette formation influenced by the menstrual cycle. The modulation of endometrial lymphocyte response to PHA or to Con A stimulation in normal fertile women during the menstrual cycle seems unrelated to the number of T cell

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00079.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:The absolute numbers of B lymphocytes and of total and “active” T lymphocytes in peripheral venous blood (Mv) from 15 females at the time of normal term deliveries were found to be significantly less (p<0.001) than in the fetal umbilical vein (Uv) or artery (Ua) or in the peripheral blood of 75 normal nonpregnant controls (Cv), suggesting that maternal cellular immunity at term is lowered. In 19 umbilical artery samples, titers of lymphocytotoxic antibodies (Cyt), expressed as the mean log of reciprocal titer values, were significantly higher (p<0.01 in each case) than in matched maternal samples, against the following cell types: Maternal T cells (7.1 in Ua vs 1.21 in Mv sera); maternal B cells (3.23 vs 1.58); T cells (4.41 vs 1.38) but not B cells from other females at delivery; autologous T cells (2.9 vs 1.0); autologous B cells (1.88 vs 0.69); T (5.39 vs 0.81) and B (2.89 vs 1.25) cells from the paired Uv; T (3.78 vs 0.62) and B (2.64 vs 0.77) cells from the Uv of other newborn infants; and T (4.19 vs 2.0) but not B cells from controls (Cv). The highest Cyt titers in the umbilical artery samples were against maternal T lymphocytes. Immunofluorescence studies indicated that the Cyt antibodies were primarily IgG. Absorption of 13 other Ua sera with maternal T cells eliminated Cyt activity against both Mv and Cv T cells; absorption with Cv T cells eliminated the reaction against Cv T while reducing Cyt titers to Mv T lymphocytes. We conclude that the fetus produces lymphocytotoxic antibody specifically directed against maternal T lymphocytes, in addition to antibody against T lymphocytes of other adu

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00080.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:The leukocytic response of the mammary gland in the locally vaccinated guinea pigs to challenge with specific antigen during lactation was investigated. The response was measured by enumerating cells in milk collected at various times prior to and following antigenic challenge. A significant leukocytosis was observed in milk from vaccinated animals. The maximum cellular response by vaccinated animals was observed at 16 h to 30 h after challenge. The majority of leukocytes collected at that time did not form EA rosettes. Differential cell counts showed that the polymorphonuclear leukocytes were the major cell type until 30 h postchallenge while the mononuclear leukocytes predominated later. The delayed cellular response to challenge and the predominancy of leukocyte type at various times after challenge are discussed. It is proposed that the leukocyte response of the mammary glands in vaccinated animals was a cell‐mediated immune reactio

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00081.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:Heterologous anti‐HCG and anti‐β‐HCG sera failed to effect complement‐dependent lysis of purified51chromium‐labeled syncytiotrophoblast in vitro. The progesterone synthetic activity of syncytiotrophoblast was also preserved in the presence of the anti‐HCG sera. Binding of the anti‐HCG antibodies to the syncytiotrophoblast was demonstrated in an immunofluorescence test. The potential of the anti‐HCG sera to participate in complement‐dependent lysis of other HCG‐bearing cell types was revealed using BeWo cells as the targets; 53%‐68% of the latter were killed by exposure to anti‐HCG sera and complement.The possible mechanism(s) whereby syncytiotrophoblast resists damage by the anti

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00082.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00083.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT:Pharmacologic immunosuppression and plasmapheresis have both been advocated as adjunctive methods of treatment for Rh immunization, but a combined regimen of the two has not been attempted. We treated four nonpregnant, severely Rh‐sensitized female volunteers with intensive plasma exchange and either promethazine 150 mg or prednisone 60 mg per day in an attempt to remove circulating anti‐Rho(D) and prevent further synthesis of the antibody. Each patient received three or four exchanges. The Rho(D) antibody titer decreased by at least one dilution immediately following 11 of 14 plasma exchanges and was ultimately lowered at least two dilutions in all patients. In one case the titer was reduced from 1:512 to 1:16, and a low titer was maintained for the duration of treatment. However, this regimen could increase the risk of infection for a mother and/or infant, as evidenced by the concomitant lowering of viral and bacterial antibody titers in these wo

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1982.tb00084.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY