摘要:

ABSTRACT:The involvement of antigens coded by the major histocompatibility complex in alloimmunization of guinea pigs with sperm has not been fully explored. Some of the “in vitro” correlates of cell‐mediated immunity, such as the lymphocyte transformation test in presence of sperm cells, could be influenced, at least partially, by histoincompatibility. Outbred Hartley and inbred strain 13 guinea pigs were alloimmunized with sperm cells emulsified in Freund's complete adjuvant. The degree of histocompatibility between recipients and sperm cell donors was determined by using mixed lymphocyte culture assays. Stimulatory indices higher than four, indicating histoincompatibility, were obtained frequently between members of Hartley strain or between Hartley and strain 13 guinea pigs, but never between strain 13 animals. Sperm cells from histoincompatible guinea pigs failed to stimulate blastogenesis in lymphoid cells from nonimmunized animals. The lymphoblastogenic potential of allo‐ and isospermatozoa were compared. Lymphoid cells from Hartley and strain 13 guinea pigs alloimmunized with sperm cells were incubated in vitro with histocompatible and histoincompatible sperm cells. Both strain 13 and Hartley sperm cells gave a similar stimulatory index with lymphoid cells belonging to outbred or inbred alloimmunized guinea pigs. The use of fresh spermatozoa was of paramount importance. Frozen specimens lost their stimulatory capacity almost completely. Within the limits of the technique utilized here, we have failed to detect any influence of the antigens coded by the major histocompatibility complex of the guinea pig upon the sperm‐induced lymphocyte transformation. These observations point to the sperm‐specific autoantigens as being responsible for the lymphocyte pr

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00279.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

ABSTRACT:Spleen cells obtained 30 days after the first immunization of rats isoimmunized with an extract of male accessory glands (MAG) were capable of adoptively transferring specific cell‐mediated immunity to normal syngenic recipients. Humoral and cell‐mediated immune responses were investigated in donor and in the recipient rats that were killed 7 days after intravenous (IV) injection of the cells. In recipient rats the cell‐mediated immunity showed multiple ways of expression and in some cases this was exemplified by a sharp increase in regard to the donor's response. Furthermore, a widespread reactivity in the spleen, lymph nodes, and thymus cells was detected. On the contrary, no circulating antibodies to MAG antigens were demonstrated after the spleen cell transfer. Cell separation studies showed that a nylon woolnonadherent cell was responsible for the transfer of the cell‐mediated immune response. This was abrogated by depletion of T lymphocytes and treatment with antirat thymocytes serum and complement. The mechanism of transfer and development of the cell‐mediated immunity in recipient rats is

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00280.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

ABSTRACT:Experimental autoimmune vesiculoprostatitis (EAVP) was transferred within inbred Wistar rats by a relatively small number of spleen cells obtained 30 days after immunization of donors with MAG extract in CFA and injected in the internal jugular vein of recipients. Specific tissue alterations identical to those present in the glands of the donors were observed 7 days after the transfer of the cells. The main alteration present was infiltration of mononuclear cells in the interstitium accompanied by fibrosis in more severe cases and flattening of epithelium in the ventral and dorsal prostate, seminal vesicle, and occasionally in coagulating gland. Nine out of ten recipients developed sexual accessory glandular lesions of various degrees. No histological alterations were observed in the absence of cell‐mediated immune response and extensive damage was only observed in rats expressing two or more positive assays. The separation of the transferred cells into enriched population of T and B cells has proved that T lymphocytes are required for the production of the tissue lesions. Depletion of T lymphocytes by nylon wool separation and anti‐rat thymocytes serum and complement completely abrogate their capacity to transfer the glandular alterations. Furthermore, specific antibody precursor cells on their own seem to be incapable to promote the antibody synthesis and to initiate the glandular damage. We conclude that T lymphocytes are required for the adoptive transfer of specific tissue damage observed in autoimmune vesiculo‐prostatitis as well as for the development of a cell‐mediated immune response to MAG antigens. Whether the same or distinct T‐cell subsets are involved in these two effects is

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00281.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

ABSTRACT:Primiparous and multiparous healthy pregnant women were tested at the end of the gestation period, or immediately after delivery, for their lymphocyte reactivity to paternal or neonatal cells in mixed lymphocyte culture and in cell‐mediate cytotoxicity assays. Freshly isolated maternal lymphocytes had no spontaneous cytotoxic activity against PHA‐activated neonatal or paternal lymphocytes. In conventional 6‐day mixed lymphocyte cultures and in cytotoxicity assays, maternal lymphocytes displayed a response similar to that of paternal or third party lymphocytes when stimulated with neonatal, paternal, or third party lymphocytes. By contrast, in 3‐day mixed lymphocyte cultures maternal cells had a selectively lower response to paternal antigens (expressed either on cord blood cells or on paternal lymphocytes), than to unrelated alloantigens. Removal of T‐lymphocytes with IgG receptors (T), or of T‐lymphocytes reacting with OKT8monoclonal antibody, corrected the depressed response of maternal cells in these 3‐day cultures. It is suggested that circulating maternal lymphocytes contain antigenspecific suppressor cells characterized by their membrane receptors for IgG and

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00282.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

ABSTRACT:We studied the effect of pregnancy plasma and monoclonal antibodies to the histocompatibility antigens HLA and DR on migration of normal leukocytes from capillaries. It was observed that plasma from women in the first trimester of pregnancy significantly enhanced leukocyte migration at a concentration of 10% (mean area of migration = 50.7 ± 9.2 cm2), as compared with plasma from nonpregnant women (22.8 ± 7.0 cm22; p<0.05). This effect was less during the second trimester (39.5 ± 3.5 cm2; p<0.05) and no enhancement was noted with plasma obtained from the third trimester of pregnancy. Similar results were obtained with a 20% concentration of plasma also. On the other hand, the monoclonal anti‐HLA and anti‐DR antibodies had an inhibitory effect on migration of leukocytes. These results are discussed in relation to the immunoregulatory role of pregnancy plasma in the nonrejection of the fetal all

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00283.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

ABSTRACT:We have made an effort to determine whether or not there is any change in subpopulations of lymphocytes in normally pregnant and preeclamptic pregnancies using monoclonal antibody markers. Eleven normally pregnant and ten women with preeclampsia were studied, both during the third trimester and again two months postpartum, and compared to eleven age‐matched nonpregnant women. Mononuclear cells were isolated from heparinized venous blood. One million cells were treated with each appropriate antibody (Ortho‐mune OKM1, OKT3, OKT4, OKT8, OKT11, OKIa), and then reacted with FITC‐antimouse IgG and examined by flow cytometry and/or fluorescence microscopy. No significant differences between these three groups were noted in the OKT3, OKT4, OKT8, OKT11, or OKIa cellular populations. The OKM1 population was significantly decreased in the third trimester of normal pregnancies but not in the preeclamptic pregnancies. No significant differences were found 2 months postp

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00284.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

ABSTRACT:Nya: NYLAR mice were immunized to murine alpha‐fetoprotein (AFP) by active immunization with rat AFP in Freund's adjuvant emulsion before mating or by passive immunization with a high or low dose of whole rabbit antimouse AFP serum or rabbit anti‐AFP IgG at 8–20 days of gestation. In the passively immunized group, anti‐AFP serum or purified anti‐AFP IgG administered at the end of the second week of gestation produced abortion after 24 hours of 41 and 48% of fetuses, respectively. Although abortion did not occur in the low‐dose group, the anti‐AFP serum produced fetal death in 32%, as did the anti‐AFP IgG in 26%, in 72 hours. In the actively immunized group rat AFP produced developmental arrest, but not abortion, in mothers bearing autologous antibodies to mouse AFP. Histopathologic analysis revealed that fetal death resulted from separation of fetal and maternal tissues of the placenta due to subplacental hemorrhages. Immunofluorescent localization of the rabbit IgG implicated both the chorioallontois and yolk sac placenta as

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00285.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

ABSTRACT:Twelve women underwent elective major gynecologic surgery for benign, non‐pregnancy‐related conditions. No perioperative or postoperative “anti‐adhesion” adjuvants were used. Serum immunoglobulin levels (IgG, IgM, IgA) and peripheral cell counts, including lymphocyte subpopulations, were measured in each patient during the immediate preoperative and postoperative periods as well as 5 to 8 weeks later. These results were compared to a treatment population of 16 women who underwent similar surgery and received 150 ml 6% dextran 70 intraperitoneally at the conclusion of the procedure. There was a decrease in the level of all serum immunoglobulins measured in the immediate postoperative period in both groups (not statistically different). At 5 to 8 weeks postoperatively there was a greater level of IgM in the control population than in the dextrantreated group (P = 0.03). Alterations in the peripheral cell counts, including lymphocyte subpopulations, were similar in the two groups. The use of intraperitoneal 6% dextran 70 did not result in postoperative alterations in the human immune system different from those induced by the surgical procedu

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00286.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0271-7352    

DOI:10.1111/j.1600-0897.1984.tb00287.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY