摘要:

AbstractThe mammographic findings and pathologic results in 219 patients biopsied after preoperative needle localization for nonpalpable breast lesions are reviewed here. Microcalcifications and masses of varying configuration are evaluated. Smooth masses showed no evidence of malignancy. Microcalcifications of five or more and stellate masses had a high incidence of malignancy. Masses with minimal irregularity not associated with microcalcifications had a low incidence of malignancy. In 63 patients in whom a breast cancer was found, excluding two initially examined because of known metastatic disease, 88% were later found to be free of disease in axillary lymph nodes.

ISSN:0735-7907    

DOI:10.3109/07357908609039821

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractMolecular alterations of cellular proteins were assessed in three previously described subpopulations of human colon carcinoma cells that were originally isolated from a single human primary colon carcinoma. The subpopulations designated HCT 116b, HCT 116, and HCT 116a exhibited significant differences in their expression of several biological properties. Immunoautoradiographic analysis of membrane components, fractionated by SDS-PAGE and electrophoretically transferred onto nitrocellulose sheets, distinguished the most aggressive tumor cell subpopulation from the intermediate and least aggressive subpopulations. An elevated expression of antigens of molecular weight in the range of 116-120 led, 92 kd, 55-66 kd, and 31 kd was found to be associated with the most aggressive subpopulation of HCT 116a cells. The binding pattern of RCA I and WGA lectins to membrane components, fractionated by SDS-PAGE and fixed on nitrocellulose, also distinguished the three subpopulations of cells. Elevated bindings of RCA 1 to 80-92 kd components of HCT 116b cells and elevated bindings of WGA to 120 kd components of HCT 116a cells distinguished the three sub-populations from one another. Analysis of cellular phosphoprotein profiles, following labeling of the cells with32Pi in the presence of phosphate-free medium, further distinguished the most aggressive subpopulation from the intermediate and least aggressive subpopulations. High level of phosphorylation of 120 kd and 250 kd nuclear components, 30 and 90 kd cytosolic components, and low level of phosphorylation of lower molecular weight membrane components were found to be associated with the most aggressive subpopulation. It is concluded that differences in the expression of membrane antigens, differences in the glycosylation of membrane components, and the selective phosphorylation and/or dephosphorylation of cellular proteins exist in subpopulations of intratumoral colonic carcinoma cells with different biological properties. These biochemical alterations of cellular proteins may play an important role in the generation of phenotypic diversity and heterogeneity of malignant cells.

ISSN:0735-7907    

DOI:10.3109/07357908609039822

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractA primary human pancreatic tumor line (BxPC-3) has been established from a biopsy specimen of a histologically confirmed adenocarcinoma of the body of the pancreas. Tumorigenicity was proven by xenograft in athymic nude mice. Upon re-establishment of tumor xenografts in tissue culture, the epithelial tumor cells retained their original morphology. Histopathologically, the tumors grown in nude mice exhibited the original characteristics of the primary adenocarcinoma in the patient, producing traceable mucin and displaying moderately well to poorly differentiated adenocarcinomas with occasional lymphocytic infiltrations at the tumor peripheries. Furthermore, the tumor xenografts differentially expressed carcinoembryonic antigen, human pancreas cancer-associated antigen, and human pancreas-specific antigen. Karyotyping and glucose-6-phosphate dehydrogenase isoenzyme characterization revealed that this tumor line was of human origin and devoid of HeLa cell contamination. The BxPC-3 tumor line has been maintained for more than four years in our laboratory and represents a valuable model for primary human pancreatic cancer.

ISSN:0735-7907    

DOI:10.3109/07357908609039823

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractSingle doses of14C-labeled bisantrene, a new antitumor agent, were administered intravenously at 10 and 100 mg/kg to mice bearing intraperitoneally implanted B16melanoma. At 24 hr after dosing, the tumors contained relatively high drug concentrations as compared to most of the other tissues. The concentrations averaged 2.4 and 28.3µg/g tumor and the tumor/blood concentration ratios were 40/1 and 29/1 for the low and high doses, respectively.

ISSN:0735-7907    

DOI:10.3109/07357908609039824

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

ISSN:0735-7907    

DOI:10.3109/07357908609039825

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

ISSN:0735-7907    

DOI:10.3109/07357908609039826

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

ISSN:0735-7907    

DOI:10.3109/07357908609039827

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

ISSN:0735-7907    

DOI:10.3109/07357908609039828

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractModern interest in cancer immunotherapy emerged in 1969 following the pioneer reports of George Mathe on the effect of Bacillus Calmette-Guerin (BCG) in suppressing murine cancer and apparently increasing survival of children with acute lymphatic leukemia (1). Mathe employed intensive repeated weekly multisite scarifications with BCG during both the induction and maintenance phases of the steadily improving chemotherapy in vogue in the late 1960s. His favorable but uncontrolled studies stimulated a number of“historically controlled”trials of intensive heavy weekly BCG treatment. In the last eight years, at least 60 randomized controlled trials and many other nonrandomized studies have been completed. These employ diverse immunomodulators including various formulations of BCG (2) and BCG derivatives [BCG-MER, BCG cell wall skeletons (CWS)], as well asCorynebacterium parvumlevamisole, thymosin, poly A-poly U, and other agents. A vast array of confusing fragmentary observations and generally nonsignificant borderline findings have been summarized in several published international symposia.

ISSN:0735-7907    

DOI:10.3109/07357908609039829

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor