摘要:

AbstractTwenty-seven evaluable patients with small cell lung cancer (SCLC) resistant to, or relapsed after induction combination chemotherapy (CT) were treated with etoposide (VPJ6) plus cisplatin (DDP). Previous treatment was: alternating CT with cyclophosphamide (C), adriamycin (A), methotrexate (M), procarbazine (P) (CAMP)/VP16, BCNU (B), hexamethylmelamine (H) (VP16BH) in 16 patients; C, A, vincristine (CAV) in 6 patients; C, A, and VP16 (CAVP16) in 5 patients. We observed 2 (7%) complete responses (CR) and 9 (33%) partial responses (PR). Duration of CRs was 8 and 14 weeks, respectively. PRs lasted a median of 22 weeks (range 16-44). Seven of 21 (33%) patients previously treated with VP16 responded to DDP plus VP16 (D-V). These results confirm D-V regimen as active in SCLC patients even when heavily pretreated. Our 33 % response in patients who had VP16 in their induction treatment regimen provides further evidence of an important potentiating effect of DDP, as reported in animal system.

ISSN:0735-7907    

DOI:10.3109/07357908809077023

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

AbstractAnalysis of estrogen and progesterone receptor proteins was carried out in 75 premenopausal and 79 postmenopausal patients with primary operable breast carcinoma who were treated from January 1983 to December 1984. The frequency of estrogen receptor protein positive/progesterone receptor protein positive (+/+); estrogen receptor protein negative/progesterone receptor protein negative (−/−); estrogen receptor protein negative/progesterone receptor protein positive (−/+); and estrogen receptor protein positive/progesterone receptor protein negative (+/−) was 40.5%, 30.5%, 23%, and 6% in premenopausal patients, respectively, and 52%, 24%, 2.5%, and 21.5% in postmenopausal patients, respectively (p<0.001). The mean positive estrogen receptor protein concentration (expressed as femtomoles per milligram of protein $ SEM) was significantly higher in postmenopausal patients (54±6) than in premenopausal patients (19±2) (p<0.005). The progesterone receptor protein values did not differ significantly between these two groups. The phase of the menstrual cycle was recorded at the time of surgery in the 75 premenopausal women. Maximum receptor positivity occurred in the secretory phase, however, this difference is not statistically significant, and our data suggest that there are no distributional differences between the phase of menses and positivity of estrogen and progesterone receptor proteins. Future studies which included analyses of circulating sex steroid levels and receptor proteins will provide a better understanding of complex hormonal regulatory mechanisms which exist in patients with breast cancer.

ISSN:0735-7907    

DOI:10.3109/07357908809077024

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

AbstractFingerprints from 200 women with histologically proven breast cancer (case group) were compared to fingerprints from 138 women with no history of any malignant disease (control group). Of the patterns analyzed, four were significantly associated with breast cancer: accidentals, transitionals, angled ulnar loops, and horizontal ulnar loops. A fifth print, the angled radial loop, was found to be of borderline importance as an independent predictor of breast cancer. Of 200 patients in the case group, 27 had one or more accidental prints, 58 had one or more transitionals, 34 had one or more horizontal ulnar bops, and 93 had one or more angled ulnar loop patterns. In 138 control subjects there were 2 with accidental patterns, 21 with one or more transitionals, 6 with horizontal ulnar loops, and 16 with one or more angled ulnar loops. In addition, there appeared to be a gradient of risk; a woman with one type of suspicious print is at higher risk of breast cancer than a woman with none, and two suspicious prints indicate a higher risk than does one. If these findings are confirmed, the prints described will represent a noninvasive anatomical marker of breast cancer risk.

ISSN:0735-7907    

DOI:10.3109/07357908809077025

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

AbstractSpleen cells from BALB/c mice immunized with mitomycin C-treated MOPC 104E plasmacytoma cells demonstrated negligible cytotoxic activity (<10% specific cytotoxic activity) in the51Cr release assay. These cells exhibited increased cytotoxic activity when they were secondarily sensitized in vitro with mitomycin C-treated MOPC 104E cells. Spleen cells from normal mice showed tumor-specific cytotoxic activity when cocultured with mitomycin C-treated tumor cells at the optimal responder to stimulator ratio of 25:1. The level of cytotoxic activity obtained by in vivo primed and secondarily in vitro sensitized spleen cells did not exceed the level of activity obtained by in vitro primary sensitized cells. Significant suppression of the cytotoxic activity of in vitro primary sensitized cells was observed when cocultured with in vivo primed spleen cells during primary sensitization in vitro at a responder to suppressor cell ratio of 1:1. Suppressor cells of in vivo primed mice were removed by treatment with anti-Thy 1.2 and complement. These results suggest that spleen cells from in vivo primed mice consisted of at least two subpopulations of cells, a cytotoxic (prekiller) and suppressor T cells. Attempts to induce cytotoxic cells in vivo might have failed because of the appearance of suppressor T cells.

ISSN:0735-7907    

DOI:10.3109/07357908809077026

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

AbstractThe effect of sodium butyrate and retinoic acid added singly or in combination on substrate-dependent growth, colonization efficiency in soft agar, and carcinoembryonic antigen (CEA) production in three human colorectal carcinoma cell lines differing in their degree of differentiation was studied. All three colon cancer cell lines regardless of their state of differentiation had their growth markedly slowed by sodium butyrate, and to a lesser extent by retinoic acid. When both agents were added together, a small synergistic inhibition of growth was noted in all the cell lines. Butyrate eliminated colony formation in soft agar in all three cell lines, however, retinoic acid only reduced colony formation in the well differentiated cell line DLD-2. Sodium butyrate was able to induce CEA production in the undifferentiated cell (MIP-101) and the moderately differentiated cells (clone D) which were previously negative for this marker. It also enhanced the baseline production of CEA in the well differentiated cells (DLD-2). Retinoic acid did not induce CEA production in clone D or MIP-101 cells, but did enhance the production of CEA in DLD-2 cells. When both retinoic acid and sodium butyrate were added together, CEA production was either additive (DLD-2) or was inhibited (clone D and MIP-101). One explanation of these results is that only well differentiated cells have functional cellular retinoic acid-binding protein (cRABP), and that certain actions of retinoic acid (inhibition of anchorage-dependent growth) are independent of the presence of cRABP.

ISSN:0735-7907    

DOI:10.3109/07357908809077027

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

AbstractThe in vivo exchange kinetics of creatine kinase in the hind leg muscle of rats containing a transplanted mammary adenocarcinoma has been investigated using31P magnetic resonance spectroscopy. Using a solenoid coil, the adenosine triphosphate (ATP) resonances arising from the tumor could be distinguished from ATP resonances arising from the muscle surrounding the tumor by use of inversion spin transfer techniques. This procedure affords a specific method of evaluating ATP metabolism of tumors in vivo.

ISSN:0735-7907    

DOI:10.3109/07357908809077028

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

AbstractIn an experiment to evaluate the merit of isopycnic centrifugation as a method of separating cell types in human head and neck squamous cell carcinomas, cells have been isolated from four specimens of these tumors and subjected to isopycnic centrifugation in continuous Percoll gradients. Cell types were identified by electron microscopy. The R- (ribosome-rich), T- (tonofilament-rich), and RT- (intermediate) cell types yielded broad bands overlapping extensively with one another, and partially with the bands of leukocytes. The pattern differed for each tumor studied, so that universal density levels separating given cell types could not be found. Isopycnic centrifugation proves less suitable in analyzing cells dispersed from solid tumors than for cells in suspended culture, blood, effusions, etc., probably because of heterogeneous growth conditions of cells in solid tumors.

ISSN:0735-7907    

DOI:10.3109/07357908809077029

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

ISSN:0735-7907    

DOI:10.3109/07357908809077030

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

ISSN:0735-7907    

DOI:10.3109/07357908809077031

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

AbstractCancer-associated lactate dehydrogenase is a tyrosylphosphorylated form of human skeletal muscle isoenzyme, since the partial amino acid sequences of human liver LDH-K/A protein were found to be identical with the known primary structure of human LDH-A isoenzyme and the LDH-A isoenzymes from human placenta and bovine muscle were shown to be tyrosylphosphorylated. This tyrosylphosphorylated LDH-K/A protein was also found to be complexed with 21 kD, 30 kD, and 56 kD proteins.

ISSN:0735-7907    

DOI:10.3109/07357908809077032

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor