摘要:

SummaryPure red cell aplasia (PRCA) is an uncommon disorder, many cases lacking a well defined aetiology. This report describes three cases of PRCA (two idiopathic and one associated with B‐CLL) who were investigated to assess the possibility of their PRCA being associated with a clonal proliferation of T‐lymphocytes. The results show that one patient had evidence of T‐cell receptor (TCR) gamma chain rearrangement, and the other had a TCR delta chain rearrangement. These two cases raise the possibility of PRCA being associated with a clonal proliferation of T‐cells and further studies are wa

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1993.tb00115.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryReference ranges for several haematology parameters in pregnancy were determined by the mathematical detection of Gaussian and Gamma distributions in partitioned but unselected patient data. For each trimester, red cell parameters were shown to be well described by Gaussian distributions. Platelet and white cell parameters were best described by Gamma distributions with the exception of eosinophil and basophil counts for which neither distribution was applicable. The reference ranges derived for each trimester are compared.

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1993.tb00116.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryBone marrow biopsy (BMB) is generally performed either on the anterior superior iliac spine (ASIS) or the posterior superior iliac spine (PSIS), the choice between these two sites depending largely upon practice at individual centres. No previous study has attempted to ascertain which of these two sites is preferable for needle BMB. We studied 72 biopsies, of which 36 were of the PSIS and 36 of the ASIS, measuring the length of the cylinder and the area of the histologic section. We asked those patients who had undergone BMB at both sites which had been the less painful. The cylinders obtained from the PSIS were found to have a significantly greater length and area than those obtained from the ASIS (P<0.00001). Of the 13 patients who underwent BMB at both sites, 11 reported the PSIS biopsy to have been distinctly less painful (P= 0.012). We conclude that needle BMB of the PSIS provides samples of greater length and area, and is less painful, than that of the ASIS.

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1993.tb00117.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryA computer‐based expert system is described which handles the output of the Technicon H1 in the haematology laboratory of a large teaching hospital. Using patient request data, analyser results, error and morphology flags, the expert system decides: whether to validate the main indices plus differential; whether a blood film is required for manual review; and which abnormal results require phoning to the requesting doctor. The results of this computer‐assisted analysis are sent to a printer adjacent to the analyser. The print‐out details any further action required of the operator before release of results to the main hospital computer, and serves as a log of all samples run on that analyser. Benefits of the expert system include greatly simplified interpretation of the large array of analyser flags, and consistency in sample handling for all operators over all s

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1993.tb00118.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryApplying thiazole orange stain and multiparameter flow‐cytometry, immature erythrocytes, e. g., reticulocytes, can be discriminated from the mature erythrocytes by virtue of their higher RNA‐content. One major problem of this method, however, consists in the inclusion of nucleated cells (leucocytes) and large RNA‐containing platelets in the population defined by light scatter‐patterns as erythrocytes. Due to the high intensity of staining with thiazole orange of all these cellular elements, all of them in the analysis are prone to be classified erroneously as reticulocytes. In order to classify elements in analysis properly as erythroid (reticulocytes + mature erythrocytes) or as non‐erythroid (leucocytes + platelets), an alternative staining method is shown in this paper, consisting of thiazole orange combined toanti‐glycophorin‐A‐PE‐mo

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1993.tb00119.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryThe preservation effect of a new platelet solution‐acidified glucose nutrient solution (AGN)‐was tested by adding AGN to pooled platelet rich plasma (PRP) prior to preparation of platelet concentrates (PC). Each PRP unit was prepared from a unit of whole blood and four PRP of the same blood group were pooled in 400 ml volume PVC bags. Equal aliquots of each pooled PRP were made prior to preparation of PC. AGN was added to one aliquot and nothing was added to the control aliquot. Equal volume and concentration PC were then prepared and the PC were further aliquoted for storage at 22°C. After five days, the parameters of platelet count, pH, aggregability and hypotonic shock response (HSR) for PC preserved in AGN plasma were better than those of the controls preserved in normal ACD plasma. The aggregability and HSR of AGN platelet concentrates recovered to baseline after two h of incubation with fresh plasma. The results of electron microscopy show that platelets preserved in AGN have less changes in morphology. The results of our work suggest that the preservation effect of AGN on PC is similar to the effect of using second generation containers or preparation of platelets from thrombocytapher

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1993.tb00120.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1993.tb00121.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1993.tb00122.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryTreatment of malignancy during pregnancy is difficult because of the potential teratogenicity of chemotherapeutic agents. Leucapheresis was employed in the initial management of a pregnant patient with chronic myeloid leukaemia. It was subsequently replaced with oral hydroxyurea during the second trimester. Successful delivery of a live male infant was achieved and the child has normal growth and development to date.

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1993.tb00123.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0141-9854    

DOI:10.1111/j.1365-2257.1993.tb00124.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY