摘要:

Within the framework of a new concept for ex vivo conditioning organs prior to transplantation, prostaglandins F2α (PGF2α) and E2 (PGE2) were investigated for their protective effects against in vitro immunologically induced morphological changes in the myocardium. Experiments were carried out with isolated rat hearts preserved at + 4 °C for 6 days with a ‘cytoplasmic’ perfusion fluid in the presence of an anti-rat serum and/or either PGF2α and PGE2. Electron microscopical investigations have shown that prostaglandins are able to prevent or to minimize remarkably myocardial lesions induced by the anti-ra

ISSN:0014-312X    

DOI:10.1159/000128217

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Unprotected and preserved myocardial ultrastructures were analyzed in prepump and postreperfused states. The experiments were divided into three groups: group 1 – normal hearts functioning 60 min in a special heart-lung model; group 2 – hearts with 30, 60, 90 and 120 min of normothermic ischemia plus 60 min of recirculation; group 3 -hearts with 30, 60, 90 and 120 min of hypothermic cardioplegia plus 60 min of recirculation. Anoxic hearts could be set in motion, but after longer anoxia the symptoms of stone-heart regularly appeared. Reperfusion induced grave ultrastructural changes both in the energy system (mitochondrial edema, rupture and lysis of cristae) and myofibrils (hypercontraction, elongation, Z line anomalies). Cardioplegic hearts showed a dynamic recovery. However, after reperfusion, characteristic sporadic signs of ischemia could always be demonstra

ISSN:0014-312X    

DOI:10.1159/000128218

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

In the course of hyperthermic perfusions of the limbs, platinum 100 Ω 1/5 DIN measurement resistances have been applied for temperature monitoring. It could be shown that application of this linear measurement arrangement (total deviation: 0.05 %) in connection with a thermoprinter can meet the most extreme precision requirement. Intra- and postoperative tissue and nerve lesions could be minimized by exact temperature monitoring

ISSN:0014-312X    

DOI:10.1159/000128219

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The effect of ethanol intoxication on haemostasis was studied by transection of mesenteric microvessels and liver resection in the rat. Plasma concentrations of alcohol were within the range of those found in ethanol intoxication in man. Bleeding time and blood loss were increased 1 h after ethanol administration, regardless of the utilized technique. A significant positive correlation existed between bleeding time following liver resection and bleeding time after simultaneous transection of a mesenteric arteriole and venule. Coagulation mechanisms, assayed by whole blood clotting time, APT time, one-stage prothrombin time, recalcification time, thrombin time, Owren’s P & P test and determination of plasma factor V and fibrinogen levels, were not significantly changed in ethanol-intoxicated animals. Administration of alcohol did not affect fibrinolytic activity, while it inhibited significantly ADP and collagen-induced platelet aggregation in the ra

ISSN:0014-312X    

DOI:10.1159/000128220

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Changes in plasma proteins and hematocrit (Ht) were studied in jaundiced rabbits subjected to hemorrhagic hypotension induced by the modified method of Wiggers and compared with changes in the hepatic energy status. At 48 h after common bile duct ligation, jaundiced rabbits showed hypoalbuminemia and a lower hepatic energy charge (EC), (ATP+1/2ADP)/(ATP+ADP+AMP). 30 min after induction of hypovolemic hypotension, in order to maintain a mean arterial pressure of 40 mm Hg, 86% of the blood collected in the reservoir (about 19 % of the normal whole blood volume) had to be successively reinfused over a 2-hour period. Despite increases in Ht and plasma total protein (p-TP) occurring in parallel with the reinfusion, plasma albumin (p-Alb) and the ratio of p-Alb to plasma globulin (A/G) did not increase. On the other hand, in sham-operated rabbits small amounts of blood had to be frequently removed during the first hour (about 34% of the normal whole blood volume), and only 34% of it had to be reinfused over a 2-hour period. Despite removal of more blood and a concomitant decrease in Ht, p-Alb and A/G ratio were higher than in jaundiced rabbits. After marked decreases in EC and total adenine nucleotides (TAN) due to the initial blood loss, EC recovered somewhat and remained higher in sham-operated rabbits than in jaundiced rabbits, whereas TAN did not recover in either group. This seems to indicate that hemodilution functions effectively to maintain microcirculation following hemorrhagic hypotension in sham-operated rabbits, while in jaundiced rabbits a rapid breakdown of protective homeostatic mechanisms results in the earlier deterioration of the hepatic energy status.

ISSN:0014-312X    

DOI:10.1159/000128221

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

This study tests the possibility that the difference in acid reduction after parietal cell vagotomy (PCV) and selective gastric vagotomy with a drainage (SGV + D) could be explained by differences in the secretion recovered rather than by real secretory differences. A method for estimating the completeness of aspiration during gastric function tests using the isotope Nai251CrO4 as an inert marker is described. The method was used in patients with the two types of vagotomy and the recovery of gastric secretion was more complete after PCV (89.7%) than after SGV + D (75.1%). This difference may substantially account for the apparent difference in reduction of acid secretion after the two operations. The pyloroplasty supplementing the SGV is probably responsible for a pyloric loss causing a lower recovery after this operation.

ISSN:0014-312X    

DOI:10.1159/000128222

出版商:S. Karger AG    

年代:1982

数据来源: Karger