ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb05091.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

House dust miteD. farinaeandDer f II‐specific IgG and IgG subclass responses were evaluated in 32 adults with perennial rhinitis undergoing immunotherapy for 1 year by means of IgG‐RAST and ELISA. The ELISA method, which is based on subclass‐specific monoclonal antibodies, could detect 0.5–1.5 ng/ml of specific antibodies.D. farinaeandDer f II‐specificIgG4antibodies increased continuously as immunotherapy proceeded, while total IgG and IgG1antibodies reached a plateau value 6 months after the start of immunotherapy, followed by a slow decrease during maintenance therapy. During the early phase of treatment the concentration of IgG, and IgG4antibodies rose, even though the increase of IgG4antibodies dominated. The positive clinical outcome as measured by the decrease in conjunctival sensitivity was associated with an increased ratio of specific IgG4/IgG, antibodies as well as the magnitude of the IgG4subclass response. Quantitation of IgG subclass antibodies in patients undergoing immunotherapy may be of some clinical value, but the clinical usefulness needs to be demonstrated for each type of allergen and possibly also for each ass

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb05092.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The presence of viable mould propagules in indoor air was investigated using the N6‐Andersen sampler in combination with DG18‐agar, in relation to house damp (characterized with a checklist) and in relation to the presence of moulds in outdoor air. The first part of the study was conducted in 46 houses in the autumn of 1987, the second part in 84 houses in May 1989. Further, in the second part, the results obtained with settlement plates (OPD) were compared with those obtained with the N6‐Andersen sampler. The number of CFU/m3in the indoor and outdoor air varied widely. A large variety of mould genera and species was isolated. Species ofCladosporium, PenicilliumandWallemiapredominated. The variability in time was high and the reproducibility of the measurements in terms of CFU/m3and of species isolated was only moderate. The low predictive value of these measurements limits their use in epidemiological studies of the relationship between exposure to moulds and respiratory symptoms. Overall, the geometric mean concentration was somewhat higher outdoors than indoors. However, the clear differences found between the number of CFU/m3belonging to different mould species in in‐ and outdoor air show that the presence of viable mould propagules in indoor air is not simply a reflection of the presence of moulds in outdoor air. The presence of moulds in indoor air was only weakly related to house damp as characterized by the checklist. High, statistically significant correlations were found between the CFU yield obtained with the OPD and the CFU/m3yield obtained with the N6‐Andersen sampler. However, the number of species isolated with the OPD was significantly lower than that obtained with the N6‐Ander

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb05093.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Two latex (Hevea brasiliensis) raw materials, one from the cytosolic fraction, the other from the total natural latex have been characterised by immunoblotting techniques. The two latex fractions A and B were studied by immunoprints using eight sera from patients who were allergic to latex. They gave different profiles though there were also allergens of common molecular weights (60, 35, 30, 25, 20, 18, 15, and 10 kD). This study confirms the importance of allergic reactions to latex, that may in some patients cause anaphylactic shock.

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb05094.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The efficacy and safety of a once‐daily dose of loratadine 10 mg (5 mg in patients whose body weight was ≤ 30 kg) and astemizole 2 mg/10 kg body weight o.d. were compared in a 14‐day third‐party blind study. Forty‐one children (30 boys and 11 girls, aged 6–14 years) with seasonal allergic rhinoconjunctivitis entered the study. The pollen count was monitored throughout the study. A significant improvement (p<0.01) in allergy symptoms was observed from the third day for both drugs; there was no significant difference between drugs, although loratadine led to a greater reduction in symptoms. In the astemizole group, the apparently rapid onset of drug action might by explained by reduced pollen exposure. Therapeutic response was excellent/good in 83.3% and 58.8% of the loratadine and astemizole groups, respectively. The results reported by the patients/parents were similar. Nine of the children on astemizole and four children on loratadine complained of side effects; three patients in the astemizole group were withdrawn from treatment because of adverse effects. No abnormal changes in lab values were observed in e

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb05095.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Development of eosinophilia was studied in four strains of guinea pigs (gp), selectively bred for either high or low respiratory anaphylactic reactivity. One high‐asthma strain (IMM/S 209) and one low‐asthma strain (IMM/R 203) developed spontaneous high blood eosinophilia. The 2 other gp strains ‐ one high‐asthma strain (IMM/S 740) and one low‐asthma strain (IMM/R 201–16) – maintained normal low levels of eosinophilic granulocytes (eos). The levels of eos in various tissues showed similar differences between the gp strains. Following immunization with ovalbumin/Al(OH)3the levels of blood eos increased significantly only in gp of strain 209. The blood eos levels in gp of all 4 strains decreased significantly following immunization with ovalbumin in Freund's complete a

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb05096.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Allergenic components of cat pelt extract fractionated by SDS‐polyacrylamide gel electrophoresis (PAGE) and transferred to nitrocellulose membranes were identified using sera from 15 allergic patients who showed positive skin test and RAST to cat extract. Two components showed the highest IgE‐binding frequency; 14 kD (being recognized by 86% of the sera examined) and 29 kD (73% of the sera tested). Both components were purified by electroelution and subjected to RAST inhibition assays. The 29 kD fraction was able to bind specific IgE toD. pteronyssinusand inhibited a RAST assay of 29 kD component (Der p I) purified from aD. pteronyssinusextract. These results could explain the finding of positive skin test with some commercial cat allergen solutions in patients withD. pteronyssinusalle

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb05097.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Assessment of efficacy in immunotherapy trials is difficult: a number of parameters can be measured; these may give conflicting results and it is not clear which best represents the clinical state. We therefore derived a Clinical Index (CI), by combining five individual parameters‐diary card symptom score, visual analogue symptom score, drug score, nasal challenge and skin prick test‐by rank analysis. The CI has been used to express the results in a trial of house dust mite (D. pteronyssinus) immunotherapy for allergic rhinitis, comparing two active treatments, Pharmalgen® and Allpyral®, with placebo. The derived index correlated well with all parameters measured. Correlations between the five individual parameters were variable: with the exception of the skin test, good correlations were seen, each parameter correlating with two or three of the others, but the visual analogue showed the closest association with other parameters. Results from a single parameter taken alone could be misleading, e.g. in the Allpyral group, nasal challenge improved significantly, but symptoms assessed either by visual analogue or diary card score did not. The CI showed significant improvement in the Pharmalgen group but not in the Allpyral group. The CI gave a more reliable measure of outcome than individual param

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb05098.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

A young woman (26 years old), with gastrointestinal candidosis of 8 months duration, was hospitalized in our department to study her cell immunity. Five minutes after an id injection of PHA (bactophytohaemoagglutinin M, Difco, 1 mg), mix monilieae, mix tricophyton and PPD Berna, she showed an extensive wheal and flare reaction in the PHA injection area, eyelid oedema and respiratory distress. In spite of adrenaline, corticosteroid and antihistamine drug administration, the patient collapsed and was admitted to the resuscitation department, where she quickly recovered. In the following days, the patient underwent immune and allergy testing: 1) total Ige immuno‐enzymatic assay: 72 KU/l; 2) specific IgE and IgE immuno‐enzymatic assay for Candida: negative; 3)in vitrolymphocyte transformation test (thymidine 2‐C14) with PHA Difco was positive. This finding and the positivity of the id injection with PHA could indicate an allergic type I immunoreaction, the description of which we have not found in the international literature (the presence of IgG STS cannot be totally excl

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb05099.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

A case of anaphylaxis related to alcohol consumption during immunotherapy is reported. We advise to consider whether there has been noticeable alcohol consumption prior to each injection.

ISSN:0105-4538    

DOI:10.1111/j.1398-9995.1992.tb05100.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY