摘要:

Summary.A new method which allows separation of the rotational and translational components of human sperm motility, based on the angular dependence of the dynamic laser light scattering (DLS) has been developed. The technique was used in a clinical study and was compared with an independent evaluation by video micrography. A good correlation was found between the two techniques when applied on different semen samples (r= 0.90;P<0.01 andr= 0.96;P<0.01 for rotation and translation, respectively) and when applied on the same semen sample at different temperatures. The rapid evaluation of these parameters using DLS technique opens the possibility to study large number of semen samples under physiological and pathological conditions at a lower cost.

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1991.tb02480.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Book reviewed in this article:G. Frajese, E. Steinberger and L.J. Rodriguez‐Rigau (Eds.):Reproductive Medicine: medical therapy.A. A. Acosta, R. J. Swanson, S. B. Ackerman, T. F. Kruger, J. A. van Zyl and R. Menkveld (Eds.):Human Spermatozoa in Assisted Reproductio

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1991.tb02481.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.Comparing motility parameters with a trans‐membrane migration method and a Hamilton‐Thorn HTM‐2000 computer assisted semen analyzer, we found that trans‐membrane migration ratio (TMMR) correlated best with critical motility which indicated the fraction of fastest and straightest sperm in a semen sample. We also found that TMMR correlated better with progressive velocity than with track speed. It is concluded that nonprogressive sperm were not included in the estimation of TMMR and the trans‐membrane migration method is most suitable for studying drug effect on straight and rapid sperm

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1991.tb02482.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.Six different techniques were evaluated for improving the accuracy and efficiency of the sperm penetration assay (SPA). Flushing and puncture of the oviduct were found to result in similar recovery of eggs from the oviduct, percentage of penetrated eggs, and penetrations per egg. However, the time required to flush the oviduct tended to be longer than for the puncture technique. Exposure of eggs to a 5% formalin solution prior to mounting resulted in fewer ruptured eggs, greater recovery of eggs, and a reduced time to mount and read the eggs compared to eggs not exposed to formalin. The percentage of penetrated eggs and penetrations per egg were similar for formalin exposed and nonexposed eggs. Significantly fewer penetrated eggs and penetrations per egg were noted when eggs were read as fresh mounts compared to eggs that were fixed and stained prior to evaluation. A significantly longer time was also required to read the freshly mounted eggs compared with the stained eggs. The results of these studies indicate that to maximize the accuracy and efficiency of the SPA, cumulus masses should be removed from the oviducts by puncture, eggs should be placed in a 5% formalin solution before mounting on slides, and eggs should be fixed and stained prior to evaluating for penetrations.

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1991.tb02483.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Book reviewed in this article:Keith L. Moore:Embryologie. Lehrbuch und Atlas der Entwicklungsgeschichte des Menschen.A. Negro‐Vilar, A. Isidori, J. Paulson, R. Abdelmassih and M. P. P. De Castro (Eds.):Andrology and Human Reproductio

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1991.tb02484.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.The simultaneous swimming up and down from identical semen specimens suggested that the separation efficiency of progressively motile sperm by the swim‐down method was superior to that of the swim‐up method. The swimming down was performed in a small plastic funnel, the bottom of which was plugged with a 2.5 ml disposable syringe. In this funnel, 2.0 ml of 70% Percoll was poured and then the sperm resuspension was overlaid. After 1 h, progressively motile sperm penetrating into the lower part of Percoll layer (1.6 ml) was collected in the syringe. The technical strategies to yield higher density of progressively motile sperm were found to be centrifugal concentration of the sperm from whole ejaculate prior to the swimming down, and subsequent re‐centrifugation of the separated sperm after the swimming down. Oligo‐asthenozoospermic semen (22 ± 4.4 × 106ml 5.5 ± 4.4% motillity,n= 8) was processed by the present method, yielding sperm qualities of 32 ± 19 × 106ml−1, 74 ± 14% in the final preparations. Overall improvement in fertility index (sperm density ml−1× motility % × 10−8) reache

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1991.tb02485.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.Two different techniques of human sperm motion analysis were compared. In 25 ejaculates, diluted to an appropriate concentration, the sperm motion index called “specific progressive motility” (SPM) was assessed by a modified conventional method. The same sperm preparations were also analysed by the CellSoft® system for computer assisted sperm analysis (CASA). The SPM‐value was found to correspond mainly to the variables motility and curvilinear velocity presented by CASA (multipler= 0.92,P<0.001). Linearity of sperm progression, lateral head displacement, and beat cross frequency did not significantly contribute to the relationship between SPM and CASA. For comparative studies between SPM and CASA the logarithmic values of SPM are recom

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1991.tb02486.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.To improve the quality of cryo‐preserved sperm, ejaculated semen was concentrated prior to freezing by means of continuous‐step density gradient centrifugation. Freezing was simplified by employing liquid nitrogen vapor with KS‐II cryo‐medium. The original semen (n= 32, 41 ± 13 × 106/ml, 17 ± 11% motility) was improved by the processing to be 68 ± 29 × 106/ml, 55 ± 19% motility. Even after the specimens were thawed, sperm concentrations were similar to those of the original semen and 39 ± 18% motility remained with a mean survival rate of 74 ± 7.7%. The improved sperm motility and higher survival rate resulted in better post‐thaw sperm quality than those of t

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1991.tb02487.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.The determination of total fibrinolytic activity of ejaculates was realized by fibrin plate method. For the same seminal samples, tissue plasminogen activator (t‐PA), urinary plasminogen activator (uPA) antigens and uPA activity were specifically quantified. The seminal values were fifty times higher than in the blood for t‐PA and fifteen times for uPA. There was no correlation between the both levels but from split ejaculates measurements a higher concentration was observed in all first fractions. By zymography assays, it was shown that seminal plasminogen activators are under active forms. The lack of proUrokinase in semen was also demonstra

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1991.tb02488.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Book reviewed in this article:E. Matouschek (Hrsg.):Arzt und Tod. Verantwortung, Freiheiten und Zwänge.Friedrich Müller und Otto Seifert:Taschenbuch der medizinisch‐klinischen Diagnostik.

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1991.tb02489.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY