摘要:

Summary.The state of the acrosomal membranes in human spermatozoa was studied by means of the hypo‐osmotic swelling test and indirect immunofluorescence using anti‐boar outer acrosomal membrane antibodies. The swelling phenomenon observed in the acrosomal region was characterized by expansion of the plasma membrane without modification of the outer acrosomal membr

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02672.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.Twenty infertile patients with normal tubal patency were inseminated intraperitoneally (11 once, seven twice, and two three times) with spermatozoa (mean 14 times 106, range 0.6–48 times 106) prepared by the standard swim‐up technique. The occurrence of immunization to spermatozoa was looked for by the Gelatin Agglutination Test (GAT) and Tray Agglutination Test (TAT). Both tests gave negative results for all the controls (10 pregnant and 10 puerperal women).Antisperm antibodies were measured in the serum before, 30 d and 4–7 months after Intraperitoneal Insemination (IPI).The last check was done for only 14 patients, since six became pregnant as a consequence of the first treatment. Of the 14 patients studied after 4–7 months, seven had two, and two had three IPI.In the group of inseminated patients, 18 women with no basal sperm antibody did not show evidence of antibody formation after the treatment and it was not increased after insemination in the two patients who already had low antibody titre (1/32).In conclusion, despite the large number of spermatozoa inseminated and even after several IPI attempts, there was no evidence ofde novoproduction of or increase in already present anti‐sperm antibodies according to the methods used for the detection of ASA in t

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02673.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.Concentrations of cadmium, lead, selenium, and zinc were determined in semen and seminal plasma of 22 volunteers by atomic absorption spectrometry. Additionally conventional semen parameters and, by means of computer videomicrography, motion parameters of spermatozoa were evaluated. Concentrations of Cd, Pb, and Zn determined in semen were not significantly different from those measured in seminal plasma. However, selenium levels were significantly higher in semen (53.8 ± 22.9 μg 1−1) than in seminal plasma (40.4 ± 15.5 μg 1−1,P<0.01). The investigated semen samples on average contained low levels of Cd (0.4 ± 0.23 μg 1−1) and Pb (9.8 ± 6.5 μg 1−1). Studies on the intra‐individual variability revealed the following average coefficients of variation (%) for element concentrations: Pb (70), Cd (53), Se (27), and Zn (23); and for semen parameters: total sperm count (46), sperm concentration (37), motility (22), ejaculate volume (21), linearity (19), linear velocity (11), curvilinear velocity (10), and percentage of normally formed sperm (9). Significant positive correlations were detected between semen selenium levels and sperm concentration (r=0.51,P<0.05), and percentage of normally formed sperm (r=0.46,P<0.05), respectively. Sperm motility (r=0.53,P<0.02), linear (r= 0.76,P<0.001) and curvilinear velocity (r= 0.64,P<0.002) were significantly correlated with sem

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02674.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.By electron microscopic studies it could be demonstrated that malformed outer dense fibres are a major cause of structural, flagellar disorder in human spermatozoa. Biochemical investigations of isolated outer dense fibres revealed that these flagellar substructures consist of three major polypeptides and do not contain phosphoproteins. They seem to have passive‐elastic properties with respect to sperm motility. Further studies are required to elucidate the cause of the disturbed development of outer dense fibres, which may be due to inflammatory, toxic, or genetic influence

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02675.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.Changes in the localization of sperm surface glycocomponents of testicular, epididymal, vas deferens, and ejaculated spermatozoa of dog (Canis domesticus) were studied employing fluorescein isothiocyanate conjugated lectins viz., Concanavalin A (ConA), Triticum vulgaris (WGA), Maclura pomifera (MPA), and Arachis hypogaea (PNA) agglutinins. The plasma membrane clothing the acrosome of the testicular, epididymal, and vas deferens spermatozoa shows reactivity with all the lectins used. However, in the ejaculated spermatozoa, the entire sperm surface shows reactive sites for ConA, WGA, and PNA. Variation in the labelling of the cytoplasmic droplet in different stages of spermatozoon transit in the epididymis has been discussed.

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02676.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.Buffalo sperm heads and tails were cleaved by sonication and isolated in relatively pure proportions i.e. 95% and 98% respectively, by discontinuous sucrose density‐gradient centri‐fugation. Purified plasma membranes from the isolated sperm heads and tails were obtained by hypotonic treatment and brief sonication followed by discontinuous sucrose density‐gradient centrifugation. Ca2+, Mg2+‐ATPase activity was evident in plasma membrane from sperm heads and tails, although activity was greater in the latter. A calmodulin‐like protein isolated from buffalo seminal plasma increased the Ca2+, Mg2+‐ATPase of plasma membrane from the sperm heads and tails by 128 and 136% respectively. Based upon the data obtained here and elsewhere (Sidhu&Guraya, 1989a) a model is proposed which explains regulation of Ca2+in buffalo spermatozoa and implicates calmodulin‐like protein and Ca2+, Mg2+‐ATPase in sperm ac

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02677.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.The post‐natal growth and differentiation of the vas deferens was studied in rats aged 1–180 d by light, and with transmission electron microscopy in parallel with a radioimmunoassay of serum testosterone. During the first week and the major part of the second week of the post‐natal life the vas deferens presented embryological features. Differentiation of the proximal and distal segments of the vas started 12 d after birth. At 25 d, the epithelium of the mucosa in the distal segment already showed some adult histologic characteristics. The weight notably increased between 1 and 45 d and the higher rates of growth of their histologic components occurred until 45 or 60 d, in parallel to testosterone serum levels. These results suggest that growth and differentiation of the vas deferens are androgen‐dependent events and precede the end of the first spermatogenic cycle, assuring the viability of spermatozoa that will enter t

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02678.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.The effects of cyproterone acetate (CPA), an antiandrogenic steroid, on the ultrastructure of the epithelial cells of the human epididymis were studied. Alterations in the cell size, in the cytoplasmic and surface characteristics, and in the morphology of different organelles are described.The different ultrastructural changes observed suggest that the absorptive and secretory functions of the principal cells are impaired following the CPA treatment. By contrast, with the drastic response of the principal cells of the ductuli efferentes and caput epididymidis, the epithelial cells of the cauda epididymidis appeared less affected. The suggestion of a differential androgen dependence among the different regions of this androgen target organ, as well as between the major cell‐types of the epididymal epithelium, is briefly discusse

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02679.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.Palomo's operative method was employed in the surgical treatment of 58 cases with infertile varicocele, and the operative method and therapeutic results were assessed.With the lapse of time after operation, statistically significant improvement was achieved in the sperm density and sperm motility. The postoperative impregnation rate was 55.2%. When the pre‐operative sperm motility index had been 20% or less, the post‐operative impregnation rate was 23%, which was significantly lower than the overall rate of 55.2%. Thus, the pre‐operative sperm motility index is a good indicator of the post‐operative capacity for impregnation. Even in patients with severe oligozoospermia (lt;5 times 106ml−1), a post‐operative impregnation rate of 45.5% was obtained. Thus, their prognosis is by no means hopeless. Study of the cross‐sectional profile of the internal spermatic vessels revealed the possibility of overlooking small internal spermatic venous branches. Therefore, Palomo's method was concluded to be excellent as an approach performed under direct visual observation.Palomo's method did not cause any complication of testicular atrophy and improved the fertil

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02680.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02681.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY