摘要:

A synthetic polynucleotide (TG)nwas hybridized to equine DNA digested withHinfIand hypervariable hybridization patterns were obtained. Mendelian inheritance of these DNA fingerprinting patterns was confirmed by pedigree analysis. Estimates of the probabilities of identical band patterns in unrelated individuals of different breeds (Swedish Trotters, North Swedish Trotters, Thoroughbreds and Arabians) were in the range 1 times 10‐4‐7 times 10‐6The variability derived with the (TG)n, probe in horses was higher than what we obtained with several other commmonly used probes for DNA fingerprinting. Individuals within breeds tended to be more similar to each other with regard to DNA fingerprint pattern than to individuals of other breeds. Moreover, a parsimony analysis made on the basis of the hybridization patterns gave clustering of individuals within breeds. The possibility of using hypervariable probes for the identification of breed‐specific characters is di

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00226.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Previously published allelic frequencies at four polymorphic protein coding loci were used as a basis for examining genetic relationships among 19 European populations of Atlantic salmon,Salmo salar L.‐ exclusive of Baltic drainages ‐ from the Barents Sea to Spain. The data did not support a model of distinct ancestral (e.g. Boreal and Celtic) origins, but were consistent with all populations descending from a single ancestral group within this region with genetically diverged populations drawn together through limited local migrati

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00227.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Brush borders or enterocytes obtained from the small intestine of 248 pedigreed pigs were tested by adhesion assayin vitrowith enterotoxigenicEscherichia(E.). coli strains, each expressing one of the three K88 pilus variants K88ab, K88ac and K88ad. All pigs were classified as belonging to one of the four adhesion phenotypes: I ‐ K88ab(‐), ac(‐), ad(‐); II‐K88ab(‐), ac(‐), ad(+); III‐K88ab(+), ac(+), ad(‐); and IV‐K88ab(+), ac(+), ad(+). Serum or red cells were typed for 15 blood group systems: A‐O, B, C, D, E, F, G, H, I, J, K, L, M, N and O; for 11 biochemical polymorphisms: PI1, PI2, PO1A, A1BG, GPI, PGD, TF, HPX, ADA, PGM and AMY; the polymorphism at the IGHG1 locus. Linkage analysis was performed between the alleles at the locus (loci) specifying K88 receptors able to bind one or more different serological types of K88 E. coli and alleles for markers at other loci. Linkage was demonstrated between the locus for the L blood group system and the locus (loci) for K88E. colireceptors (Ẑ= 3.24), adding one locus (loci) to the previously identified linkage group IV (LGIV) [L‐SLB]. The maximum likelihood estimate of the recombination fraction (0C) was 0.23. No evidence was found for linkage between any of the other biochemical and immunogenetic markers and the receptor lo

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00228.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

A sheep kappa cDNA probe was isolated, characterized by sequence analysis and shown to have significant sequence identity to other kappa light chains. This probe and a sheep lambda light chain probe were used to estimate the extent of various sheep immunoglobulin light chain gene loci by Southern blot analysis of genomic DNA. The results showed that the sheep has a single hybridizing kappa constant gene and three to five kappa V segment bands. Segregation of three polymorphic bands at the lambda C locus indicated that they were products of separate C segments. Restriction fragment pattern variations were obtained using light chain probes on various sheep breeds, but no pattern or individual band was characteristic for a particular breed.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00229.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

In situ hybridization techniques were used to localize regionally the calcium release channel (CRC) gene on cattle and horse chromosomes, using a porcine CRC cDNA probe. In cattle, the hybridization signal peaked on the 18q23‐q26 bands and in horse on the 10pter region. Previous studies have shown that the glucose phosphate isomerase (GPI) gene localizes at the same site in both species, indicating that the two loci are syntenic. As CRC and GPI are syntenic in human, pig and mouse, the present results in cattle and horse represent another example of synteny conservation in the evolution of mammalian chromosome

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00230.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

A panel of bovine‐murine hybrid cell lines was analysed for 10 loci, including three (IGF1, IGHG2 and the calcium release channel gene [CRC]) that have previously been mapped in man, but not in cattle. The IGF and CRC genes were indirectly mapped to chromosomes 5 and 18 respectively and the syntenies of the HOX2 and GH genes and of the NP and FOS genes were confirmed. The results also show that the IGHG2 locus, which is linked to NP and FOS on human chromosome 14, is separated from these genes in cattle. By showing synteny of the IGHG2 and MPI loci, the IGHG2 locus has been indirectly mapped to chromosome 2

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00231.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Computer programs are available in the software package SAGE to perform a variety of segregation and linkage analyses used by human geneticists. These methods are designed specifically to uncover major gene segregation in pedigree data coming from non‐inbred populations. With the aid of a closely linked polymorphic marker, they can detect a locus that contributes as little as 10% to the variation of a quantitative trait in a pedigree sample of several hundred individual

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00232.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The multilocus probe pV47 detected an average of nine bands in cattle between 23 kb and 4kb. Band sharing was estimated for three groups of unrelated animals. The first group comprised 20 individuals of 12 different breeds, the second group 10 individuals of the Swiss Simmental population and the third group 11 individuals of the Swiss Brown Swiss population. The band sharing probabilities were 33%, 42% and 58% respectively. The DNA fingerprints of 38 offspring with a total of 277 bands revealed no bands that could not be traced to the parents.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00233.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The locus for apolipoprotein‐B (APOB) has been chromosomally assigned in swine byin situhybridization of a genomic probe to metaphase chromosomes. As expected based on the observation of extensive linkage conservation and based on the previous assignment of the malate dehydrogenase locus (MDH1) in swine, APOB maps to chromosome 3, specifically to region 3q24‐qter. Variations at APOB may represent both in humans and in swine risk factors for hypercholesterolaemia and atherosclerosis. Evidence presented here that the human and porcine APOB occupy evolutionarily conserved chromosome regions provides a basis for using the pig as an animal model to study the APOB associated atherosclerosis r

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00234.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

A method is described for identifying variants at the bovine β‐lactoglobulin locus by combining polymerase chain reaction (PCR) amplification and molecular hybridati

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00235.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY