摘要:

SummaryA swine genomic cosmid library constructed from a genotypically SLA homozygous Large White individual was screened with a murine genomic 21‐hydroxylase probe. A clone which contained a pig 21‐hydroxylase gene was isolated and after subcloning, the 5′ region of the gene was sequenced. The deduced amino acid sequence corresponded almost exactly to the NH2terminal portion of the steroid 21‐hydroxylase from porcine adrenal microsomes. Comparison of the first 99 amino acid residues of both sequences revealed three substitutions comprising two leucine residues in positions 10 and 13, and one arginine residue in position 55 for our sequence, instead of threonine in position 10 and lysine in position 13 and 55 for the isolated enzyme. A swine homologous probe was derived from the isolated 21‐hydroxylase gene and used for gene assignment by RFLP studies in two swine leucocyte antigen (SLA) informative families. The results demonstrate that the swine 21‐hydroxylase gene is located within or close to the swine MHC. Taken together, the present results suggest the existence of a single 21‐hydroxylase gene per h

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1990.tb03202.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryGenetic control of immune responsiveness in cattle was investigated using an antigen‐dependent T cell proliferation assayin vitro. Bovine T cell proliferative responses to ovalbumin were dependent upon major histocompatibility complex (MHC) class II molecules. Responses of an unrelated panel of animals to a limiting concentration of ovalbumin after a single immunization were compared. Two discrete patterns of response were observed. One group of animals had low or non‐responses which were not significantly different from the preimmune levels. Another group of animals showed significant responses. After a second immunization the majority of low responders remained low responders. There was no significant correlation between bovine MHC class I BoLA haplotype and magnitude of response within this group of unrelated animals. However, the magnitude of the T cell responses by two half‐sib family groups segregated with BoLA haplotypes inherited from the sire. In contrast no significant correlation with antibody responsesin vivocould be demonstrated. We suggest that the observed variation in T cell response is linked to bovine MHC class II immune response (Ir)

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1990.tb03203.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryA new hypervariable tandem repeat was isolated from the pig genome and characterized by DNA sequence. The use of this DNA fragment as a probe in order to follow allelic segregation and DNA fingerprinting in pigs, horses and rabbits is documented.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1990.tb03204.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryA monoclonal antibody (ISU‐cA) was produced that recognized certain alloantigens of the chickenAblood group locus. Antigens produced by allelesA3,A4andA8were positive, and those produced byA2andA5were negative, by haemagglutination. The specificity of ISU‐cA for chicken A blood group antigens was demonstrated by serologic analyses, genetic crosses and competitive inhibition of binding by anti‐A alloantisera. To our knowledge, this is the first reported monoclonal antibody against a chicken blood group alloantigen system other than theBco

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1990.tb03205.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryExpression of chicken red blood cell (RBC) surface antigens was studied by using a monoclonal antibody (ISU‐cA) specific for chicken A blood group antigens. Erythrocytes were examined from embryos of 3–18 days of incubation and from chicks at hatch up to 21 weeks of age. Specific antigens were detected on embryonic RBC surfaces by immunofluorescence as early as 3 days of incubation. Antigenic expression was examined by both haemagglutination and immunofluorescence and found to increase with age from embryos to mature birds. The antigen concentration on the cell surface was found to be affected by genotype; heterozygotes had an intermediate level of antigen between that of the two parental genotypes. These data confirm the co‐dominance that is observed with most blood group antigens. Flow cytometric analysis allowed confirmation that the entire erythrocyte population gradually increased in antigenic expression over time, rather than having an antigen‐negative subpopulation being replaced by a positive subpop

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1990.tb03206.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryEighty anti‐SLA class I reagents were prepared resulting from skin graft and subcutaneous immunizations in 320 fattening pigs of the Belgian Landrace and Pietrain breeds. By means of these alloantisera seven internationally and five locally established specificities were recognized. Three of the locally assigned specificities were new: BM 36, BM 37 and BM 38. They were serologically and genetically defined. The typing battery was completed with French and Danish reagents, and correlation coefficients were calculated for the main alloantisera recognizing SLA class I alloantigens observed in the Belgian breeds.The SLA haplotype frequencies were estimated in 372 Belgian Landrace and 369 Pietrain pigs. The SLA haplotype distribution differs significantly between both breeds and the genetic distance (0.54) at the SLA system is quite hig

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1990.tb03207.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryB lymphocyte alloantigens in cattle were identified by serological analysis. Alloantisera were raised by skin implant immunization or leucocyte immunization and were absorbed with platelets to reduce class I‐specific antibody activity. Leucocyte absorptions were done to reduce the complexity of some antisera. A panning technique was used to prepare B‐enriched and B‐depleted lymphocytes. Antisera which displayed anti‐B cell activity over a number of dilutions were tested against 115 Charolais cattle, and 13 antisera were used to define five B lymphocyte alloantigens. These antigens were present on B lymphocytes but did not appear to be present, at least at the same density, on the majority of T lymphocytes or platelets. Family studies suggested that these antigens are coded by one or two loci which are closely linked to the bovine class I loci. These results suggest the five antigens are class II antigens of the major histocompatibility complex (MHC) of

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1990.tb03208.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryTwo independent male‐specific DNA fragments were isolated from the bovine genome by the technique of cloning by deletion enrichment. Each is represented in the order of one to five copies per haploid genome. Neither fragment was found to be polymorphic between two individuals of different breeds with 12 different restriction enzymes. A ‘zoo blot’ demonstrated that one fragment had a closely related sequence in the sheep genome, although no other hybridizing sequences were detected in a variety of animal DNAs tested with both probes. The DNA sequence of each fragment is presented; one fragment has a continuous open reading frame and a potential Z‐DNA forming

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1990.tb03209.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryTheC3polymorphism of equine serum or plasma revealed by agarose gel electrophoresis can be diagnosed with protein stain following acid protein fixation. In addition to the three alleles previously described (C31, C32, C33), a fourth allele (C34) was found. Population data for 25 domestic breeds andEquus przewalskiiare presented.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1990.tb03210.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryGenetic distances among five of the most important Italian native sheep breeds were estimated by using gene frequencies of four blood group and seven blood protein loci and by three different computing methods. The values of the three distance matrices were highly correlated. Genetic distances were found within the range values reported in the literature for local breeds. Shorter distances fitted the historical evidence of migrations.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1990.tb03211.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY