ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb01908.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb01909.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb01910.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb01911.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb01912.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Summary.Two monoclonal antibodies (mAbs), CC17 and IL‐A67, which are specific for the bovine equivalent of the CD5 antigen, Bo5, were each found to react with the cells of some animals but not others. The cattle tested were all positive for one or both of the mAbs, but the level of expression on cells expressing both determinants was slightly lower than that on cells expressing either of the determinants on their own. Both mAbs precipitated an antigen of 67kD. However, sequential immunoprecipitation experiments with cells that reacted with both mAbs demonstrated that the determinants are present on two different sets of molecules. These findings suggested that the mAbs recognize two co‐dominantly expressed allelic forms of Bo5. This was confirmed in family studies, with groups of full‐ and half‐sibling offspring of sires and dams of defined phenotypes. These experiments also showed that the gene encoding the Bo5 antigen is not linked to the major histo‐compatibility complex (MHC). The frequencies of the two alleles, which have been designated Bo5.1 and Bo5.2, in the cattle populations tested were 100% and 0%, respectively, inBos taurus, and 10% and 90%, respectively, inBo

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00891.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Summary.A genomic clone containing a bovine DRβ‐like gene,BoDRβ II, was isolated from a bovine genomic library and characterized by restriction enzyme mapping and nucleotide sequencing of exon regions. Alignment of this sequence with the human DRβ cDNA sequence allowed identification of exon/intron boundaries. The clone contains a 13.3‐kilobase (kb) insert, and includes 1.3kb 5′ of the β1exon and 6.7kb 3′ of the transmembrane (TM) exon. Open reading frames were present in the BoDRβ exons sequenced. Nucleotide identities of the bovine β1, β2and TM exons with the corresponding human DRβ exons were 73, 91 and 83%, respectively. Nucleotide identities of these exons with those of a previously described bovine DRβ‐like pseudogene,BoDRβ I, were 69, 95 and 81%, respectively. Although a limited amount of sequence data was obtained for the intron regions, a 71% identity was found within a 514‐nucleotide region immediately 3′ to the β2exons inBoDRβ IandBoDRβ II. A series of GT residues followed by a longer series of GA residues began about 35 nucleotides 3′ of the β1e

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00892.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Summary.The influence of miniature swine major histocompatibility complex genes (SLA) upon phagocytic and bactericidal activities of peripheral blood monocytes againstSalmonella typhimuriumandStaphylococcus aureuswas measuredin vitrousing cultured cells and bacterial/enzyme‐dependent tetrazolium dye (MTT) reduction. Haplotype significantly influenced uptake and killing of each bacterium by monocytes of 4‐ and 8‐week‐old pigs. Cells from 4‐week‐old SLA ad and aa pigs were significantly better than all others at phagocytizingS. aureusand cells from dg and gg were poorest. Killing ofS. aureuswas highest at 4 weeks in SLA cd pigs and in dg and gg pigs at 8 weeks of age. Uptake and killing ofS. typhimuriumwas highest in homozygous aa and cc haplotypes at 4 weeks and pigs with the c x d recombinant haplotype had highest uptake and killing ofS. typhimuriumat 8 weeks. Litter, but not sire, also influenced significantly the uptake and killing ofS. auresandS.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00893.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Summary.New DNA sequence polymorphisms were identified at four bovine autosomal loci: growth hormone, low density lipoprotein receptor, α‐subunit of glycoprotein hormones and thyroglobulin. Assuming independent assortment between these polymorphisms, the probabilities to be heterozygous at these four loci are 0.48, 0.36, 0.10 and 0.77 respectively, within the Belgian Blue Cattle breed (BBCB). Nucleotide diversity was estimated, showing that animals from the BBCB are heterozygous for 1/1450 nucleotides, a value significantly lower than the 1/500 value found in man. Moreover, we have estimated that the mutation rate at the cytosines of CG dinucleotides is about 10 times higher than that for other nucleotid

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00894.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Summary.Plasma samples of alpacas and llamas were analysed by a simple method of two‐dimensional (2‐D) agarose gel (pH 8.6)‐horizontal polyacrylamide gel (pH 9.0) electrophoresis, followed by general protein staining of gels. Genetic polymorphism in both species is described for α1B‐glycoprotein (α1B) and three other unidentified proteins designated prealbumin (Pr), postalbumin 1 and 2 (Pal and Pa2). α1B was identified by cross‐reactivity with antisera for human and pig α1B. Altogether, two alleles of Pr, two of Pa1, five of α1B and three of Pa2 are described. Most of the alleles were present in alpacas and llamas. Alpacas showed a high degree of polymorphism at all four loci. Llamas showed considerable polymorphism at only thePa1andPa2loci. The theoretical probability of exclusion (Pe) of an incorrectly assigned parent was estimated to be about 80% in each species by typing for the six polymorphic plasma proteins reported so far in these species. The given method of 2‐D electrophoresis revealed no fixed differences in protein mobilities that discriminate between ll

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00895.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY