摘要:

Summary.A horse family in which a recombination occurred in the chromosome region coding for the serological specificities of the ELA complex and those of the A blood group system of a mare was further analysed by mixed lymphocyte reaction (MLR) and Southern blot hybridization. This family consisted of a stallion, a mare and five full sibs. The stallion and the mare were heterozygous for internationally recognized ELA specificities while only the mare was heterozygous for the A blood group system. MLR between all members of the family confirmed that the stallion possessed two different ELA haplotypes and suggested that recombination in the mare occurred outside the segment delimited by the ELA‐A locus and the MLR region. DNA samples from all individuals were investigated by Southern blot analysis using three restriction enzymes(EcoRI,HindIII orTaqI), three human HLA probes (one of class I cDNA and two of class II probes), one cDNA (DRß) and one genomic (DQα). Class I and class II restriction fragments of the mare segregated in accordance to the ELA specificities and thus clearly confirming that the crossing‐over did not occur between the ELA‐A gene and the class I, class II region nor between DRß and DQα subsets. The A blood group genetic determinants would thus be situated outside the ELA region defined by class I and class

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1988.tb00782.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Summary.Milk and blood samples were obtained from three Icelandic dairy herds. The herds were monitored regularly for mastitis incidence. Cell counts, adenosine triphosphate (ATP) and antitrypsin levels of the milk samples were recorded. In addition, red cell and BoLA typing were performed on the blood. Although cell counts and ATP levels showed significant associations with mastitis, antitrypsin levels did not. Red blood cell antigens N'2and S1and the lymphocyte antigen detected by the monoclonal antibody M7 were associated with low cell counts, whilst BoLA w6 and w6.1 were associated with high cell counts. BoLA w6.2 and w11 showed significant association with high antitrypsin levels. Only ED116 showed a significant association with mastitis.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1988.tb00783.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Summary.An account is given of the serologically defined class I specificities encoded by the bovine MHC (expressed as the BoLA system) in two populations of African cattle and in European breeds.The BoLA typing was performed using alloantisera raised against tissue antigens of both European and African breeds of cattle. All of the specificities agreed in the first two international BoLA workshops were found in the African cattle, although there were significant differences in the frequency of some specificities between the African and European animals. Many of the European antisera, which are operationally monospecific inBos tauruscattle, were multispecific in the African animals.Subgroups of two specificities (w8 and w10) were demonstrated. Five new BoLA‐A locus alleles were detected by means of antisera raised against alloantigens of African cattle. Two of these occurred at an extremely high frequency in the African populations; one being unique to these cattle. Monoclonal antibodies proved to be useful typing reagents, particularly in the elucidation of subgroup

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1988.tb00784.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Summary.Variants of serum transferrin in Japanese wild pig,Sus scrofa leucomystax,and Ryukyu wild pig,S.s. riukiuanus,of Japan were investigated by using starch gel electrophoresis. Five phenotypes, TfB, BC, C, CX and X, were observed, of which two, TfCX and X, are new variants. Comparison of gene frequency estimates which were calculated for each population showed remarkable geographic differences among several populations of these two subspecies.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1988.tb00785.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Summary.Genetic polymorphism of the ‘X’‐protein in red cells from Malaysian Katjang goats was demonstrated by starch gel electrophoresis at pH 7.3. Two new phenotypes were observed, suggesting that one new allele is involved. A new nomenclature for the ‘X’‐protein system in goats

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1988.tb00786.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Summary.Family data are presented for a new allele (Aabdg) in the A system of horse erythrocyte alloantigens which includes factors Aa and Ab traditionally thought to be products of allelic genes. Evidence for incorrect assignment of the codominant factor Ac in the presence of Ab and Ac and the absence of Aa is discussed.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1988.tb00787.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Summary.A fourth allele at the horse erythrocyte phosphohexose isomerase(Phi)locus was proposed to account for phenotypes observed after starch gel electrophoresis and enzymatic staining of red cell lysates from American Saddlebred and Tennessee Walking Horse breeds. The gene was rare, having an estimated frequency of 0.009 in 949 Saddlebreds tested.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1988.tb00788.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Summary.By using a bovine K‐Cn cDNA as probe and thePstI endonuclease we demonstrate that the DNA restriction patterns of K‐Cn AAand K‐Cn BBcows are different. Besides two invariant fragments (about 6.8kb and 1.1 kb) the former shows two fragments of about 4.3 kb and 0.3 kb and the latter one fragment of about 4.6kb. K‐CnAB cows show intermediate pattern. Therefore, it is possible to determine the bovine K‐Cngenotypes even in absence of gen

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1988.tb00789.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Summary.A new variant of glucose phosphate isomerase (GPI), also known as phosphohexose isomerase (PHI), was detected in a primitive pig population.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1988.tb00790.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Summary.By the use of isoelectric focusing in polyacrylamide gels and the Immobiline method at pH range 7.1–7.7, three haemoglobin (Hb) phenotypes were observed in Norwegian Red Cattle. Two one‐band phenotypes named HbA4and HbA6occurred in unequal proportions in their two‐band combination type with the cathodal HbA6being the stronger. In 76 dam‐offspring pairs the distribution of phenotypes were in agreement with codominant single gene inheritance. In an unselected material of 140 animals, gene frequencies were HbA4=0.94 and Hb

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1988.tb00791.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY