ISSN:1062-3345    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

Intratumoral microvessel density (iMVD) has been reported as an independent prognosticator for breast carcinoma patients. iMVD should be measured in areas of highest neovascularization (“hot spots”). Yet, measuring iMVD in the hot spot yields good, albeit variable, results due to subjectivity in identifying the hot spot. The aim of this study was to compare iMVD determinations by light microscopy (LM) between two pathologists of different experience and by a computer-aided image analysis system (CIAS). Also, CIAS determined the total microvessel area (MVA) and the total microvessel perimeter (MVP) within the same field. Microvessels from 91 node-negative, invasive ductal breast carcinomas were highlighted with anti-CD31. Median patient follow-up was 66.3 months (range 3–99). Because of limited deaths from carcinoma, survival analysis was restricted to relapse-free survival (RFS). LM-measured iMVDs correlated between pathologists (p = 0.0001) but with moderate variability (r2= 0.4). Also, LM-measured iMVDs correlated with MVA (p = 0.0003) and MVP (p = 0.0001). By univariate analysis, the LM-measured iMVD determined by the experienced pathologist (p = 0.002), MVA (p = 0.009), MVP (p = 0.032), and tumor diameter (p = 0.005) were associated with RFS. Multivariate analysis revealed that tumor diameter (p = 0.0016), LM-measured iMVD by the experienced pathologist (p = 0.0062), and MVA (p = 0.0445) were independently associated with RFS. For inexperienced pathologists, computer-aided iMVD measurement, which measures not only iMVD but also MVA and MVP, may be a more objective way to quantify the angiogenic activity of tumors.

ISSN:1062-3345    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

Immunohistochemical detection of estrogen receptors was performed in 257 primary breast carcinomas using the ER1D5 monoclonal antibody on paraffin sections. The purpose of the present study was to investigate the potential predictive or prognostic role of ER1D5 staining in breast cancer. Two hundred fifty-seven (105 node negative and 152 node positive) patients, from the Hospitals of Trento and Milano-Niguarda, were studied. Survival analysis was performed in various subsets of homogeneously treated patients using the Kaplan-Meier method. ER1D5 immunoreactivity was prognostically relevant both for relapse-free survival (RFS) and overall survival (OS) in the entire series. No statistically significant difference in survival was observed in node-negative patients. Conversely, in the node-positive subgroup, the patients with ERlD5-positive tumors showed a higher probability of 5-year survival (73.9% vs. 55.2%;p= 0.01 land a lower probability of relapse of disease (32.8% vs. 56.5%;p= 0.002) than did the patients with ER1D5-negative tumors. When subgroups of homogeneously treated patients were considered, ER status was not discriminant for RFS and OS in untreated node-negative (93 cases) and node-positive chemotherapy-treated patients (84 cases). Conversely, ER1D5 staining was prognostically relevant in a group of node-positive patients treated with tamoxifen (65 cases). The present results confirm that the monoclonal antibody can be used to define ER status on paraffin-embedded tumors and that this assay provides predictive information useful for the selection of patients who may benefit from hormonal treatment.

ISSN:1062-3345    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

Fifty-one randomly selected cases of carcinoma of the breast were stained at the same time by using an immunocytochemical method and an in situ hybridization reaction. In the former method, two antibodies (polyclonal and monoclonal) against gross cystic disease fluid protein-15 were employed. An mRNA probe against the sequence of the same protein was used. The antisera gave superimposable results in terms of specificity and sensitivity. In situ hybridization gave a specific reaction, but the sensitivity was inferior to the immunocytochemical method. This difference was probably the consequence of the different information provided by the two methods.

ISSN:1062-3345    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

We tested the hypothesis that the coordinate expression of cytokeratin 7 (CK 7) and cytokeratin 20 (CK 20) could distinguish among carcinomas arising from different primary sites. A total of 384 cases of carcinomas primary to various organs, as well as 16 cases of malignant mesothelioma, were evaluated using commercially available monoclonal antibodies and an avidin-biotin immunoperoxidase technique. The subset of tumors strongly expressing both CK 7 and CK 20 included virtually all bladder transitional cell carcinomas and the majority of pancreatic adenocarcinomas; the tumors negative for both CK 7 and CK 20 were largely restricted to hepatocellular, prostate, and renal cell carcinomas in addition to squamous cell and neuroendocrine carcinomas of lung. The CK 7-/CK 20+ immunophenotype, however, was highly characteristic of adenocarcinomas of colorectal origin, whereas CK 7 + /CK 20− immunophenotype was typically seen in the vast majority of carcinomas arising from other sites, including ovary, endometrium, breast, and lung, as well as malignant mesothelioma. Gastric carcinomas were the most heterogeneous subgroup with respect to CK 7/CK 20 immunophenotype. In the subset of mucinous tumors, striking immunophenotypic differences were noted among those primary to the breast (CK 7 + /CK 20-), gastrointestinal tract (CK 7-/CK 20+), and ovary (CK 7 + /CK 20 + ). In all cases investigated, this CK immunophenotype was invariant in metastatic vs. primary tumors. It is concluded that, in the appropriate clinical setting, the CK 7/CK 20 immunophenotype of carcinomas is a valuable diagnostic marker in the determination of primary site of origin.

ISSN:1062-3345    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

Whether hemangiopericytoma (HPC) of the dura is a variant of meningioma or a distinct entity is a controversial issue. We examined the immunohistochemical reactivity for CD34 and epithelial membrane antigen (EMA) of 20 dural-based HPCs from 17 patients and compared the results with 25 meningiomas from 25 patients (five meningothelial. nine transitional, seven fibrous, two atypical, one psammomatous, and one secretory). Histologically, the HPCs could be divided into round, spindle, and “pseudopapillary” cell types; two tumors were mixed. Eighteen of the 20 HPCs stained strongly and diffusely with CD34, whereas only two of 20 were focally positive for EMA. In contrast, only one of 25 meningiomas was focally positive for CD34, whereas 25 of 25 were positive for EMA. We conclude that immunohistochemistry using CD34 and EMA is helpful in distinguishing HPC and meningioma, and CD34 is a sensitive marker for HPC.

ISSN:1062-3345    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

HBME-1 monoclonal antibody has recently become available as a suggested immunohistochemical tool for the positive identification of malignant mesothelioma. In this study, the HBME-1 antibody was immunohistochemically evaluated on selected normal tissues and in 540 epithelial and nonepithelial tumors of different organs. Most mesotheliomas (25 of 29) showed a strong reactivity, with either a luminal or a cytoplasmic pattern. However, less differentiated mesotheliomas with sarcomatous features as well as sarcomatous areas of otherwise more differentiated mesotheliomas were negative. With regard to carcinomas, about half of the adenocarcinomas of the lung showed reactivity that was usually less conspicuous than the reactivity observed in mesothelioma. Consistently positive adenocarcinomas included endometrial and ovarian serous carcinomas. Thyroid lesions reacted differently: papillary and follicular carcinomas were strongly positive, whereas follicular adenomas and normal tissues were usually negative, suggesting that HBME-1 may be helpful in the evaluation of thyroid lesions. While gastric and pancreatic adenocarcinomas showed positivity in a minority of cases, tumors from the breast only rarely showed significant reactivity. Adenocarcinomas of colon, kidney, and prostate were almost invariably negative. Among soft-tissue and bone tumors, chordoma and cartilaginous tumors were strongly positive, and synovial sarcoma showed reactivity in the epithelial component. Other sarcomas and melanomas were negative. On the basis of our findings, HBME-1 monoclonal antibody is helpful in the differential diagnosis of mesothelioma, but because adenocarcinomas of many sites, including lung, are sometimes strongly positive, this antibody has to be used in a panel with other antibodies. We believe that the HBME-1 antibody could replace one of the antibodies that is typically negative in mesothelioma and positive in carcinoma. Interestingly, HBME-1 appears to be useful in subtyping lesions of certain organs, primarily thyroid tumors, soft-tissue and bone tumors of cartilaginous differentiation, and those with epithelial traits.

ISSN:1062-3345    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

This study describes the results of immunohistochemical staining for latent membrane protein-1 (LMP-1) in 17 patients with posttransplant lymphoproliferative disease (PTLD). The diagnosis of PTLD was based on histopathological examination and in situ hybridization for Epstein-Barr virus (EBV)-encoded small RNA (EBER-1). Expression of LMP-1 protein was found in 13 of 17 cases. indicating that the diagnostic sensitivity of LMP-1 immunohistochemistry for PTLD in formalin-fixed paraffin-embedded tissues is 76.5%. LMP-1 staining was found in large atypical, intermediate-sized, and small lymphocytes. The pattern of staining was cytoplasmic. with characteristic membrane accentuation. An occasional cell showed paranuclear staining, suggesting localization to the Golgi apparatus. LMP expression was seen in polymorphous nonclonal (9/13) and monomorphous clonal (3/13) lesions. On clinical follow-up, refractory PTLD leading to death occurred in 6 of 13 LMP-1-positive and 3 of 4 LMP-1-negative cases. Thus, PTLD lesions with immunohistochemically demonstratable LMP-1 are heterogenous with regard to biological behavior.

ISSN:1062-3345    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

Lymphocyte antigen studies are frequently employed to confirm diagnoses of mycosis fungoides (MF). Antigenic abnormalities have been reported in other diseases that show some features of malignancy such as lymphomatoid papulosis, parapsoriasis en plaque and pityriasis lichenoides; however, the range of diseases in which these lymphocyte abnormalities may appear has not been fully described. We retrospectively examined histologic and immunoperoxidase (IPOX) data from the last 265 biopsies submitted to rule out MF. In 22 (8.3%) of the cases studied, IPOX demonstrated a lymphocyte surface antigen abnormality suggestive of MF without routine histologic evidence for the diagnosis. Twelve of these patients were subsequently found to have MF. The other 10 cases (3.8% of cases examined) provide the basis for this study. Three cases had lymphoproliferative disorders in which antigen abnormalities have been previously described: two cases were thought to be parapsoriasis, and there was a single case of lymphomatoid papulosis. The histologic diagnosis was dermal hypersensitivity reaction in three cases; psoriasis (n = 1), atopic dermatitis (n = 1), seborrheic dermatitis (n = 1), and annular elastolytic granuloma (n = 1). Lymphocyte surface antigen abnormalities have not been reported in tissue studies from these seven inflammatory dermatoses with no known malignant potential. It is important to recognize that lymphocyte surface markers can rarely be altered in benign reactive conditions.

ISSN:1062-3345    

出版商:OVID    

年代:1995

数据来源: OVID

摘要:

We analyzed immunohistochemical expression of the p53 protein in 28 cases of human normal placentas, ranging from first-trimester to term, and 23 cases of trophoblastic diseases, including hydatidiform mole, invasive hydatidiform mole, and choriocarcinoma, using the labeled streptavidin-biotin (LSAB) method after heat-induced antigen retrieval. The positive reaction was restricted to the nuclei of cytotrophoblasts and intermediate trophoblasts, while the syncytiotrophoblasts showed rare immunolocalization. The majority of first-trimester (eight of 11) and second-trimester (four of five) placentas showed minimal (<5%,+) to mild (5–20%, 2+) positive reactions, whereas all cases of third-trimester and term placentas failed to stain. On the contrary, in the trophoblastic diseases, all cases of hydatiform mole evidenced varying degrees of staining from a minimal to a marked (>40%, 4+) reaction, and all cases of invasive hydatidiform mole showed minimal to moderate (20–40%, 3+) reactions. As a whole, the p53 staining was found to be more intense and higher in number in the trophoblasts of moles than in those of normal placentas. In the cases of choriocarcinoma, 50% (three of six) of the cases showed minimal to moderate staining. From our observation, it may be suggested that expression of the p53 protein could be correlated with proliferation and differentiation of the trophoblasts, especially in an early stage of pregnancy, and that expression of the p53 protein could also play a significant role in an early developmental stage of molar pregnancy.

ISSN:1062-3345    

出版商:OVID    

年代:1995

数据来源: OVID