ISSN:1424-8832    

DOI:10.1159/000214233

出版商:S. Karger AG    

年代:1978

数据来源: Karger

ISSN:1424-8832    

DOI:10.1159/000214234

出版商:S. Karger AG    

年代:1978

数据来源: Karger

ISSN:1424-8832    

DOI:10.1159/000214235

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

Chromogenic peptide substrates for serine proteases have been designed by using two approaches: (1) by using the natural substrate as a model and (2) by structure-activity correlations obtained through screening of a large number of tripeptides. Some recent examples, substrates for kallikreins and urokinase are given.

ISSN:1424-8832    

DOI:10.1159/000214236

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

A method for assaying plasma prekallikrein has been developed applying the chromogenic substrate Chromozym PK. Different variables have been investigated, and the final assay system was found to give a reproducible and reliable assay procedure. A normal value of 99.0 ± 18% was found, the coefficient of variation being 7.9. Studies on material from patients with various liver diseases indicated that the main site of prekallikrein biosynthesis is the liver

ISSN:1424-8832    

DOI:10.1159/000214237

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

New chromogenic tripeptide substrates have been used for the determination of kallikreins and urokinase. The conditions have been optimized. It is possible to determine prekallikrein in plasma after activation with Cephotest®. No significant loss in activity caused by plasma kallikrein inhibitors is observed at the dilutions used

ISSN:1424-8832    

DOI:10.1159/000214238

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

Plasma levels of prekallikrein, kallikrein, high molecular weight kininogen and kallikrein inhibitors were measured in samples from dogs infused with E. coli endotoxin. Markedly reduced levels of prekallikrein and high molecular weight kininogen were seen at circulatory collapse and free kallikrein was detected. Both ‘fast-reacting’ and ‘time-dependent’ inhibitors of plasma kallikrein were significantly reduced. Fractionation of pre-infusion and collapse samples by gel filtration together with immunological procedures indicated that both α2-macroglobulin and α1-antitrypsin were the major kallikrein inhibitors in dog plasma, and that they were significantly reduced in the collaps

ISSN:1424-8832    

DOI:10.1159/000214239

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

Chromogenic substrates were used to assay prekallikrein, prothrombin and factor X. Plasma prekallikrein was contact activated and allowed the splitting of HD-Pro-Phe-Arg-PNA which has a high affinity for plasma kallikrein. Prothrombin was assayed in various ways (immunologically, after activation with Ecarin or in a less specific way). Determination of factor X is considered as a possible specific method in the monitoring of coumarol therapy.

ISSN:1424-8832    

DOI:10.1159/000214240

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

The factor-Xa-sensitive substrate Bz-Ile-Glu-Gly-Arg-p-nitroanilide has been made more sensitive by making ester and amide derivatives of the γ-carboxyl group of the glutamyl residue. The morpholinyl and piperidyl amides react 2.5 times more rapidly with factor Xa

ISSN:1424-8832    

DOI:10.1159/000214241

出版商:S. Karger AG    

年代:1978

数据来源: Karger

摘要:

The methods of studying the specificity of synthetic substrates towards certain coagulation factors as well as the results of such studies are reviewed.

ISSN:1424-8832    

DOI:10.1159/000214242

出版商:S. Karger AG    

年代:1978

数据来源: Karger