ISSN:0882-8245    

DOI:10.1089/vim.1995.8.1

年代:1995

数据来源: MAL

摘要:

ABSTRACTThe ability of a highly divergent antigenic site of foot-and-mouth disease virus (FMDV) of serotype C to elicit neutralizing antibodies has been evaluated in mice and rabbits. The viruses compared, FMDV C-S8c1 and HR, differ in a single amino acid replacement in their capsid proteins, but represent two extreme antigenic specificities of the major antigenic site A of FMDV type C. Both, studies of cross-neutralization of homologous and heterologous virus, and fractionation of site A-specific antibodies by immunoaffinity chromatography suggest a similar immunodominance of antigenic site A in FMDV C-S8c1 and variant HR. This information is relevant to the formulation of synthetic peptide vaccines that ideally should consist of mixtures of peptides representing several antigenic specificities. These cocktail formulations may be required to control diseases caused by FMDV and, generally, by highly variable RNA viruses, since single specificity peptides may trigger selection of vaccine-escape viral mutants.

ISSN:0882-8245    

DOI:10.1089/vim.1995.8.11

年代:1995

数据来源: MAL

摘要:

ABSTRACTThe lymphocyte proliferative response to BHV-1 in immune cattle was compared to recombinant wild-type gD and truncated gD produced from recombinant vaccinia viruses. The response exhibited by recombinant proteins was comparable to the response induced by BHV-1 suggesting that gD is the major target structure for stimulation of bovine lymphocytes. Analysis of the proliferative response using vaccinia virus vectors expressing various modified forms of gD identified a region between residues 165 and 216 recognized by T-lymphocytes of immune cattle. Further analysis by overlapping peptides in this region localized the T cell epitope to residues 161–172. Antibody-blocking studies demonstrated that lymphocytes responding to this epitope are CD4+. In addition, lymphocytes stimulated with gD or peptide 77 (residues 161–172) also produced IFN-γ and

ISSN:0882-8245    

DOI:10.1089/vim.1995.8.19

年代:1995

数据来源: MAL

摘要:

ABSTRACTThe development and persistence of virus-specific antibodies were investigated in eight cattle experimentally infected with the R29 isolate of bovine immunodeficiency-like virus (BIV). By 4 weeks postinoculation (p.i.), antibodies reactive to BIVgag- andenv-encoded recombinant fusion proteins were detectable by immunoblotting in all animals. By 40 weeks p.i., seven of eight cattle had dramatically decreased Gag-specific antibodies, and anti-Gag reactivity remained very low or undetectable through 190 weeks p.i. Immunoprecipitation experiments revealed a similar loss of reactivity to nondenatured BIV Gag in these animals. In contrast, antibodies to a recombinant BIV Env protein were readily detectable throughout the study in all eight cattle. During the period of declining Gag antibody, infectious virus was recoverable from peripheral blood mononuclear cells of each animal. However, there was no evidence for sufficient amounts of BIV p26-containing immune complexes to explain the loss of anti-Gag reactivity. Interestingly, the single animal that maintained detectable anti-Gag reactivity throughout the study was repeatedly negative for virus recovery beyond 17 weeks p.i. All animals have remained clinically normal for over 4 years p.i., with no evidence of consistent changes in mononuclear cell subsets. These findings provide evidence that in BIV infection an early decline in Gag-specific antibody reactivity can occur without evidence of increasing viral replication or progression to overt clinical disease.

ISSN:0882-8245    

DOI:10.1089/vim.1995.8.27

年代:1995

数据来源: MAL

摘要:

ABSTRACTRecently isolated escape mutants of human respiratory syncytial virus (HRSV) are described. The mutants were selected after serial passage of the Long strain in the presence of monoclonal antibodies directed against the attachment (G) glycoprotein. The genetic changes associated to the mutant phenotype were nucleotide substitutions leading to either amino acid replacements or new stop codons that shorten the G polypeptide by one amino acid. Sequence changes within the three C-terminal residues of the G molecule abolished multiple epitopes, some of them being distinguished only by virus-binding inhibition of the corresponding antibodies with a panel of antiidiotypic antisera. These results extend previous studies that demonstrated the extreme capacity of HRSV to accommodate multiple sequence changes within the antigenically relevant G protein C-terminal third. These results are discussed in terms of both the antigenic structure of the G molecule and the generation of new antigenic variants that mimic natural variants of HRSV.

ISSN:0882-8245    

DOI:10.1089/vim.1995.8.37

年代:1995

数据来源: MAL

摘要:

ABSTRACTA neutralization enzyme-linked immunosorbent (Nt-ELISA) assay for determination of protective immunity to measles virus was developed and evaluated. This procedure uses the same initial steps as performed to determine antibody titers by seroneutralization (Nt) test. However, a reduction in virus infectivity by neutralizing antibody was determined by quantitation of viral antigen using ELISA. The serum dilution that resulted in neutralization of 50% of infectious virus could be determined from the absorbance values. To be able to screen a large number of specimens, the conditions of the Nt-ELISA test were adjusted such that negative sera for measles antibodies and the positive ones were clearly distinguished on the basis of a single dilution (1:4). This test showed similar sensitivity (88.3%) and equal specificity as the Nt test when screening 136 serum samples from normal subjects. The estimation of protective antibody titers by Nt and Nt-ELISA methods was strongly correlated (correlation coefficient = 0.91). Thus, the measles Nt-ELISA test is rapid, reproducible, sensitive, and specific for detection of protective measles antibodies.

ISSN:0882-8245    

DOI:10.1089/vim.1995.8.47

年代:1995

数据来源: MAL