ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1986.tb02312.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

An IFN‐resistant subline (U‐266rα) was established from the IFN‐α‐sensitive myeloma cell line U‐266 by subculturing U‐266 cells with increasing doses of INF‐α. The U‐266rαsecreted IgE at a higher rate than the U‐266 (7.2 × 10−13g/c/8 h as compared to 3.3 × 10−13g/c/8 h). The 2 cell lines were found to be equally high producers of β2m (9.2 and 9.6 × 10−13g/c/8 h). The U‐266 produced 2.9 times less IgE and 5 times more β2m compared to the initial production rates at establishment. INF‐α and recombinant IFN‐αM2(rIFN‐α2) inhibited proliferation and concomitantly decreased the rate of IgE and β2m secretion in U‐266 but not in U‐266 IFNrα, which in contrast was slightly stimulated by IFN‐α with respect to growth, IgE and β2m secretion. In addition, IFN‐α at a concentration of 100 U/ml was shown to decrease the IgE and β2m production without exerting more than minimal cytotoxicity on U‐266 cells. No antiproliferative effect was found for IFN‐γ or recombinant IFN‐γ (rIFN‐γ) on either of the 2 cell lines. IFN‐γ and rIFN‐γ were, however, found to stimulate the production of β2m. Our results show that the U‐266 and the derived IFN‐α‐resistant subline can be used as models for studying some of the biological effects of IFN‐α and ‐γ in vitro. The clinical implications of these in vitro results, in particular the usefulness of serum determinations of

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1986.tb02313.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Neutrophil chemiluminescence (CL) as a measure of oxygen‐dependent killing activity was evaluated in 3 groups of patients: (a) 63 patients with multiple myeloma (MM); (b) 31 subjects with monoclonal gammopathy of undetermined significance (MGUS); (c) 32 healthy controls. Neutrophil CL response was shown to be significant reduced both in patients with MM (p<0.001) and in subjects with MGUS (p<0.001). A significant difference was also observed between the results obtained in MM and those of MGUS (p<0.001). Treated MM patients showed a more severe impairment of neutrophil chemiluminesccence response than that observed in untreated patients (p<0.001). It is suggested that the impairment of neutrophil CL response, possibly related to decreased killing activity, may play a role, along with other known causes, in the increased susceptibility to infection observed in MM patient

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1986.tb02314.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Sera from 104 individuals splenectomized because of abdominal trauma were examined for the occurrence of 9 different autoantibodies. Our findings indicate that the occurrence of autoantibodies in splenectomized individuals without systemic disease is not increased and does not differ significantly from that found in an age‐ and sex‐matched control populat

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1986.tb02315.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Cerebrospinal fluid (CSF) beta‐2‐microglobulin (B2m) has been proposed as a marker of central nervous system (CNS) involvement in myelo‐lymphoproliferative diseases. Recently its reliability has been put in question because of false positive and false negative results. In our study, B2m was measured in 574 CSF samples collected from 74 patients affected by ALL, ANLL or lymphomas; 20 of these patients had CNS‐involvement while they were under observation. There was a significant difference in CSF B2m between the patients with and without CNS‐involvement (p<0.001). No false positive or false negative results were obtained. In 4 cases the rising of CSF B2m was observed 8, 6, 4 and 4 wk before the clinical and laboratory diagnosis of CNS‐involvement. In all patients the clinical and laboratory improvement of the neurological disease was associated with a progressive decrease of CSF B2m. Some hypotheses about the origin of CSF B2m are discussed. The authors conclude that CSF B2m is a useful and reliable marker of CNS‐involvement in myelo‐lymphoprolif

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1986.tb02316.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

We report our results with pipobroman (PB) therapy in patients with essential thrombocythemia (ET). 21 consecutive untreated patients were treated with PB from 1975 to 1984. PB was given at a dose of 1 mg/kg/d until platelet count dropped below 600 × 109/l. In 18 patients (86%) a hematological remission was obtained. Median duration of induction phase was 49 d. In all cases a maintenance regimen was required at a dose ranging from 0.2 mg/kg/d to 0.5 mg/kg/d, according to platelet number. Follow‐up of responder patients ranged from 6 to 108 months (median 17 months). Treatment was well tolerated and we observed only a very moderate and transient hematological toxicity. No patient had relapsed or developed secondary neoplasms at the time of writing. Median survival time of all patients was 24 months (range 10–

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1986.tb02317.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

10 asymptomatic young male patients with moderate splenomegaly detected at a routine examination are presented. The history and clinical examination failed to reveal the aetiology of the splenomegaly. Further investigations, including screening for blood dyscrasias, clotting abnormalities and reticuloendothelial abnormalities were likewise unrevealing. Liver biopsies, rectal biopsies for bilharzia and bone marrow aspirates for Gaucher's Disease were found to be normal. Serology for malaria and Ebstein Barr Virus infection was also negative. Positive immunofluorescent tests for IgG antibodies specific for cytomegalovirus were found in 5 patients. We consider that these patients have splenomegaly which is not of a specific nature, but may be associated with a severe antigeneic response to the previous cytomegalovirus infection. In view of the otherwise negative findings these patients should be considered to have ‘True Idiopathic Splenomegaly’, a term which would indicate the benign nature of the splenic enlargement. This diagnosis should be considered in the differential diagnosis of asymptomatic patients who have splenomegaly of undetermined ori

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1986.tb02318.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Lymphocyte subpopulations were measured in the blood of 17 patients with megaloblastic anaemia due to vitamin B‐12 deficiency. 14 patients had pernicious anaemia and 3 others were gastrectomized. By using monoclonal antibodies recognizing T cell surface markers and immunofluorescence microscopy, we found a significant decrease in the number of circulating suppressor T cells and an increase in the ratio of helper to suppressor T lymphocytes in pernicious anaemia patients. This finding may be related to other immune abnormalities found in pernicious anaemia, e.g. the presence of multiple autoantibodie

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1986.tb02319.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

A 60‐yr‐old female presented with typical thrombotic thrombocytopenic purpura (TTP). She remained in coma with frequent seizures for 1 wk, with persisting severe thrombocytopenia and microangiopathic haemolytic anaemia, despite treatment with prednisolone, plasma exchange, fresh frozen plasma, sulphinpyrazone and dipyridamole. Splenectomy induced haematological improvement within 1 d, there was cessation of fitting after 2 d, and full neurological recovery ensued over 3 wk. Laboratory studies did not reveal the presence of a platelet‐aggregating factor (PAF), stated to be present in some two‐thirds of cases. While plasma exchange and plasma infusion are beneficial in many cases, splenectomy appears still to be of value in unresponsive

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1986.tb02320.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The immunological phenotypes of leukemia cell samples from 60 patients, of whom 54 had acute myeloid leukemia (AML), were assessed with a panel of monoclonal antibodies (Mabs) with specificity for the following epitopes: (i) Epitopes associated with myeloid leukemia cells, (ii) Epitopes expressed only on immature myeloid cells (or subsets) and on monocytes, (iii) Epitopes only expressed on granulocytes or on granulocytes and mature myeloid cells (promyelocytes, myelocytes and monocytes), (iv) Epitopes on HLA‐class II (DR) and (v) HLA‐class I molecules and on insulin receptors.This panel of Mabs proved useful to identify leukemia cells in blood and to assess their myeloid origin. The panel of Mabs was found also to be useful for immu‐nophenotyping of leukemia cells. Furthermore, the analysis revealed considerable variations in the immunological phenotype of AML cells, reflecting antigenic heterogeneity within the individual leukemia cell population as well as abnormal or no expression of histocompatibility antigens and insulin receptors in some samples. Some of the Mabs bound preferentially to subgroups in the French‐American‐British (FAB) class

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1986.tb02321.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY